Navigation Links
Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived,,,Tissue

In situ Adapter for the RoboCycler 40 Temperature Cycler allows rapid amplification of rare mRNA

Donald D. Lorimer * Robert E. Carroll * Richard V. Benya
Department of Medicine, University of Illinois at Chicago

In situ adapter system for the RoboCycler 40 temperature cycler is designed for holding standard microscope slides.

Reverse transcriptase in situ PCR (RTISPCR) is a recently described method for detecting mRNAs in archival paraffin tissue blocks. This method is readily performed using the RoboCycler 40 Temperature Cycler# and its microscope slide attachment, the in situ Adapter for the RoboCycler 40 Temperature Cycler. In this article, we describe our RTISPCR method for detecting the rare message for the gastrin-releasing peptide (GRP) receptor expressed by a previously resected gastrointestinal tract tumor. By detecting mRNAs in tissues that have been maintained in paraffin blocks and archived for over 50 years, this technology promises to dramatically expand the use of PCR amplification.

The GRP receptor is a member of the 7-transmembranespanning, G protein-coupled receptor superfamily. Within the gastrointestinal (GI) tract, GRP receptors are normally expressed by smooth muscle cells and by nerve endings, acting to regulate intestinal motility and to induce the secretion of other enteric peptide hormones.1-3 However, GRP receptors are not expressed by mucosal epithelial cells lining the normal GI tract in the few mammals studied, including dogs4 and humans.5 In contrast, recent studies have shown that GRP receptors are aberrantly expressed by a number of human colon cancers6 and colon cancer cell lines including HT29, MC2
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
2. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
3. Detecting miRNA & siRNA
4. Detecting Attomole Amounts of Small RNA
5. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
6. Efficient Primary Structure Elucidation of Disulfide-bridged Peptides
7. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
8. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
9. Complex Peptide Mixture Analysis on the Finnigan LTQ Linear Ion Trap Mass Spectrometer
10. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
11. LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures
Post Your Comments:
(Date:9/30/2014)... , Sept. 30, 2014 CORD:USE Cord Blood ... announce that it has entered into an Equity and ... which has licensed a series of patented technologies for the ... possible clinical applications.  Tianhe is performing Phase I/II clinical ... Spain using Tianhe,s Stem Cell Educator ...
(Date:9/30/2014)... Basel, Switzerland (PRWEB) September 30, 2014 ... advanced software solutions for drug discovery and ... the FUNGITECT consortium has chosen Genedata Selector ... next-generation sequencing (NGS) data. FUNGITECT is a ... of invasive fungal diseases via individualized anti-fungal ...
(Date:9/30/2014)... CORALVILLE, Iowa , Sept. 30, 2014 /PRNewswire/ ... in the discovery and development of proprietary new ... United States Patent and Trademark Office (USPTO) issued ... patent application entitled, "Benzoic Acid, Benzoic Acid Derivatives ... Methods of Making and Use Thereof." ...
(Date:9/30/2014)... DEL MAR, Calif. , Sept. 30, 2014 /PRNewswire/ ... devices for women,s health announces its receipt of a ... The FemmyCycle menstrual cup has also received FDA clearance ... Health Canada, and the CE in Europe. This patent is ... design. In conjunction with this issuance, the company is releasing ...
Breaking Biology Technology:CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 2CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 3CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 4FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 3KemPharm, Inc. Receives Patent from the USPTO for Novel Prodrug of Hydromorphone, KP511 2The FemmyCycle 2
... The Board of Directors of Schering-Plough Corporation (NYSE: SGP ... share. Payment will be made on Aug. 31, 2009, to ... 2009. As of March 31, 2009, there were 1,628,335,093 common ... also declared a quarterly dividend of $3.75 per share on the ...
... BASEL, Switzerland, June 23 Syngenta announced today the successful ... year maturity. The proceeds of the bond will be used ... said: "We have taken advantage of favorable current borrowing conditions ... bond issue is part of our normal funding requirements and ...
... SyntheMed, Inc. (OTC Bulletin Board: SYMD), a biomaterials ... products, announced today that the company was recently nominated ... in pediatrics. The company is among three ... device analysts in recognition of the development of REPEL-CV(TM) ...
Cached Biology Technology:Syngenta Issues EUR 500 Million Eurobond 2SyntheMed Receives Frost & Sullivan Health Innovation Award Nomination 2
(Date:9/30/2014)... hope: A new research report published in October 2014 ... explains how scientists developed a synthetic version of a ... in human and mouse eggs similar to the natural ... fertilization. , "We believe that the results of this ... of human fertilization by providing a precise answer to ...
(Date:9/30/2014)... suite of perilous threats in today,s ocean. From ... change, fragile coral ecosystems are disappearing at unprecedented ... species of corals surrounding the island of Moorea ... their tropical environment: coral guard-crabs. New research from ... Station scientist Seabird McKeon and the museum,s predoctoral ...
(Date:9/29/2014)... of gut bacteria called Clostridium ramosum , coupled with ... work is published this week in mBio , the ... , A research team from the German Institute of Human ... bacteria including C. ramosum gained weight when fed ... ramosum were less obese even when consuming a high-fat ...
Breaking Biology News(10 mins):Synthetic sperm protein raises the chance for successful in vitro fertilization 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Gut bacteria promote obesity in mice 2
... microscope integrates two Finnish innovations and brings new dimensions ... for Molecular Medicine Finland (FIMM) have in collaboration with ... finger gesture controlled microscope. The method is a combination ... multitouch display. The result is an entirely new ...
... Congress on Osteoporosis & Osteoarthritis in Valencia, Spain, Professor ... Pierre D. Delmas Prize. The prestigious award, ... Aspects of Osteoporosis and Osteoarthritis (ESCEO) and the International ... an individual who has made outstanding and major scientific ...
... - A lot of chatter goes on inside each one ... ears. Researchers at McMaster University discovered that the "cross-talk" ... important role in the development of psychiatric illness, intestinal diseases ... "The wave of the future is full of opportunity as ...
Cached Biology News:Big size multitouch display turned into a microscope 2ESCEO-IOF Servier Pierre D. Delmas Prize awarded to John. A. Kanis 2'Knowing it in your gut' is real 2
...
TNM-FH Insect Medium 1 liter...
D.R. Harper (1993) • Includes: essential information on taxonomy, culture and safe handling of viruses, immunology, monoclonal antibodies, antivirals and vaccines....
IHC Polymer Detection Kit (Rabbit DAB)150 slides...
Biology Products: