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Detecting Attomole Amounts of Small RNA

1.Differential Recovery of Small RNAs During Total RNA Isolation. Total RNA was isolated from 1x106 HeLa cells using three standard RNA isolation procedures: single phenol/guanidinium extraction (RNAwiz), glass fiber filter (RNAqueous) purification, or double phenol extraction (ToTALLY RNA). Purified RNA samples were analyzed on a 1% denaturing agarose gel and recovery of messenger RNA (-actin, GAPDH), small nuclear RNA (U1 snRNA), and miRNA (miR-16, miR-22) were assessed by Northern blot or solution hybridization with the mirVana miRNA Detection Kit


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Cat# Product Name Size 1552 mirVana miRNA Detection Kit 100 rxns
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