( 1.Differential Recovery of Small RNAs ...)Detecting Attomole Amounts of Small RNA

1.Differential Recovery of Small RNAs During Total RNA Isolation. Total RNA was isolated from 1x10⁶ HeLa cells using three standard RNA isolation procedures: single phenol/guanidinium extraction (RNAwiz), glass fiber filter (RNAqueous) purification, or double phenol extraction (ToTALLY RNA). Purified RNA samples were analyzed on a 1% denaturing agarose gel and recovery of messenger RNA (-actin, GAPDH), small nuclear RNA (U1 snRNA), and miRNA (miR-16, miR-22) were assessed by Northern blot or solution hybridization with the mirVana miRNA Detection Kit

back to top

Ordering Information
Cat# Product Name Size 1552 mirVana miRNA Detection Kit 100 rxns
'"/>

Source:

Page: All 1 2 3
Related biology technology :

1. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
2. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
3. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
4. Detecting miRNA & siRNA
5. Constructing Directional cDNA Libraries from Limited Amounts of RNA
6. Rapidly Synthesize Large Amounts of Capped RNA
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. New Tools for Small RNA Analysis
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids