Navigation Links
Detecting Attomole Amounts of Small RNA

mirVana miRNA Detection Kit


Sensitive -- Detect miRNA or siRNA in as little as 10 ng total RNA

Specific -- Extremely low background with no need to optimize hybridization or washing conditions

Simple and fast -- Single tube procedure eliminates the need to transfer to membrane for hybridization

Multiple target detection -- Detect small RNAs and mRNAs in the same sample

The new mirVana miRNA Detection Kit provides a faster and more sensitive alternative to Northern analysis for detecting small RNAs. With this patented technology, quantitation of small RNA species can be performed with as little as 10-50 ng of total RNA. The assay is incredibly sensitive and is able to detect attomole (10-18 mol) amounts of target RNA. In addition, the mirVana miRNA Detection Kit can be used to simultaneously detect several small RNAs of the same size or both small RNA and long RNA species in the same sample. This means you can now quantitate an siRNA and its target mRNA in the same sample. (See several applications of this kit in Detecting miRNA & siRNA.)


Rapid Procedure Based on Solution Hybridization
The mirVana miRNA Detection Kit is based on a simple solution hybridization principle. The sample RNA containing the target RNA(s) of interest is mixed with one or more high specific activity radiolabeled RN A probes and the included Hybridization Buffer. After heat denaturation, each mixture is incubated at 42C to hybridize the probe to its complementary RNA molecules. Unhybridized RNA species and excess RNA probe is then removed by a rapid ribonuclease digestion step. The hybridized, protected RNA fragments are recovered in the same tube using Ambion's patented single step technology for simultaneous ribonuclease inactivation and nucleic acid precipitation. RNA samples are then resuspended with the provided Gel Loading Buffer and analyzed on a denaturing polyacrylamide gel. Because the hybridization is performed in solution, the procedure ensures a sensitive and linear detection signal after autoradiography. Furthermore, the included Hybridization Buffer has been specifically developed to provide the optimal sensitivity and specificity of detection with short antisense probes. Thus the procedure is ideal for detecting small RNA molecules such as siRNA or miRNA.


Complete Kit with Included Controls
Each mirVana miRNA Detection Kit provides enough reagents for 100 reactions. The kit also contains a control transcription template and Probe Elution Buffer to prepare and gel purify a 32 nt RNA probe specific for miR-16 miRNA. When used with the provided Mouse Kidney Total RNA, this probe generates a 22 nt protected fragment. The kit also comes with a detailed Instruction Manual and comprehensive information about experimental setup and probe design/preparation.

Figure 1.Differential Recovery of Small RNAs During Total RNA Isolation. Total RNA was isolated from 1x106 HeLa cells using three standard RNA isolation procedures: single phenol/guanidinium extraction (RNAwiz), glass fiber filter (RNAqueous) purification, or double phenol extraction (ToTALLY RNA). Purified RNA samples were analyzed on a 1% denaturing agarose gel and recovery of messenger RNA (-actin, GAPDH), small nuclear RNA (U1 snRNA), and miRNA (miR-16, miR-22) were assessed by Northern blot or solution hybridization with the mirVana miRNA Detection Kit


back to top


Ordering Information
Cat# Product Name Size 1552 mirVana miRNA Detection Kit 100 rxns
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
2. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
3. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
4. Detecting miRNA & siRNA
5. Constructing Directional cDNA Libraries from Limited Amounts of RNA
6. Rapidly Synthesize Large Amounts of Capped RNA
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. New Tools for Small RNA Analysis
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/24/2017)... and ROCKVILLE, Md. , March ... of Maxwell Biotech Venture Fund (MBVF), today announced positive ... added to the standard drug therapy regimen in patients ... small molecule drug discovered by scientists at Sequella, Inc. ... Institutes of Health. A total of ...
(Date:3/24/2017)... 2017 Agenus Inc. (NASDAQ: AGEN), an immuno-oncology ... cancer vaccines, today announced participation at the following conferences: ... Blair and Maidstone Life Sciences conference "Cancer Immunotherapy Conference" ... New York, NY . Agenus will participate ... 9:40 am: Robert B. Stein , M.D., Ph.D., ...
(Date:3/23/2017)... , March 23, 2017  SeraCare Life ... global in vitro diagnostics manufacturers and clinical ... industry,s first multiplexed Inherited Cancer reference ... by next-generation sequencing (NGS). The Seraseq™ Inherited ... with input from industry experts to validate ...
(Date:3/23/2017)... 23, 2017 NetworkNewsWire Editorial Coverage  ... Cancer remains ... strain on health care systems, in terms of costs and ... too does the development of innovative and efficient therapies that ... the many types of cancer treatments, a growing number of ...
Breaking Biology Technology:
(Date:3/23/2017)... Research and Markets has announced the addition of the ... Forecast to 2025" report to their offering. ... The Global Vehicle Anti-Theft System ... over the next decade to reach approximately $14.21 billion by 2025. ... forecasts for all the given segments on global as well as ...
(Date:3/20/2017)... At this year,s CeBIT Chancellor Dr. Angela Merkel ... came to the DERMALOG stand together with the Japanese Prime Minster Shinzo ... At the largest German biometrics company the two government leaders could see ... as well as DERMALOG´s multi-biometrics system.   Continue ... ...
(Date:3/9/2017)... , March 9, 2017 4Dx has publicly ... Lung Imaging Workshop at the University of Pennsylvania. Founder ... to deliver the latest data to world leaders in ... brings together leaders at the forefront of the industry ... imaging. "The quality of the imaging ...
Breaking Biology News(10 mins):