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Detecting Attomole Amounts of Small RNA

mirVana miRNA Detection Kit


Sensitive -- Detect miRNA or siRNA in as little as 10 ng total RNA

Specific -- Extremely low background with no need to optimize hybridization or washing conditions

Simple and fast -- Single tube procedure eliminates the need to transfer to membrane for hybridization

Multiple target detection -- Detect small RNAs and mRNAs in the same sample

The new mirVana miRNA Detection Kit provides a faster and more sensitive alternative to Northern analysis for detecting small RNAs. With this patented technology, quantitation of small RNA species can be performed with as little as 10-50 ng of total RNA. The assay is incredibly sensitive and is able to detect attomole (10-18 mol) amounts of target RNA. In addition, the mirVana miRNA Detection Kit can be used to simultaneously detect several small RNAs of the same size or both small RNA and long RNA species in the same sample. This means you can now quantitate an siRNA and its target mRNA in the same sample. (See several applications of this kit in Detecting miRNA & siRNA.)


Rapid Procedure Based on Solution Hybridization
The mirVana miRNA Detection Kit is based on a simple solution hybridization principle. The sample RNA containing the target RNA(s) of interest is mixed with one or more high specific activity radiolabeled RN
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