Navigation Links
Detecting Attomole Amounts of Small RNA

mirVana miRNA Detection Kit


Sensitive -- Detect miRNA or siRNA in as little as 10 ng total RNA

Specific -- Extremely low background with no need to optimize hybridization or washing conditions

Simple and fast -- Single tube procedure eliminates the need to transfer to membrane for hybridization

Multiple target detection -- Detect small RNAs and mRNAs in the same sample

The new mirVana miRNA Detection Kit provides a faster and more sensitive alternative to Northern analysis for detecting small RNAs. With this patented technology, quantitation of small RNA species can be performed with as little as 10-50 ng of total RNA. The assay is incredibly sensitive and is able to detect attomole (10-18 mol) amounts of target RNA. In addition, the mirVana miRNA Detection Kit can be used to simultaneously detect several small RNAs of the same size or both small RNA and long RNA species in the same sample. This means you can now quantitate an siRNA and its target mRNA in the same sample. (See several applications of this kit in Detecting miRNA & siRNA.)


Rapid Procedure Based on Solution Hybridization
The mirVana miRNA Detection Kit is based on a simple solution hybridization principle. The sample RNA containing the target RNA(s) of interest is mixed with one or more high specific activity radiolabeled RNA probes and the included Hybridization Buffer. After heat denaturation, each mixture is incubated at 42C to hybridize the probe to its complementary RNA molecules. Unhybridized RNA species and excess RNA probe is then removed by a rapid ribonuclease digestion step. The hybridized, protected RNA fragments are recovered in the same tube using Ambion's patented single step technology for simultaneous ribonuclease inactivation and nucleic acid precipitation. RNA samples are then resuspended with the provided Gel Loading Buffer and analyzed on a denaturing polyacrylamide gel. Because the hybridization is performed in solution, the procedure ensures a sensitive and linear detection signal after autoradiography. Furthermore, the included Hybridization Buffer has been specifically developed to provide the optimal sensitivity and specificity of detection with short antisense probes. Thus the procedure is ideal for detecting small RNA molecules such as siRNA or miRNA.


Complete Kit with Included Controls
Each mirVana miRNA Detection Kit provides enough reagents for 100 reactions. The kit also contains a control transcription template and Probe Elution Buffer to prepare and gel purify a 32 nt RNA probe specific for miR-16 miRNA. When used with the provided Mouse Kidney Total RNA, this probe generates a 22 nt protected fragment. The kit also comes with a detailed Instruction Manual and comprehensive information about experimental setup and probe design/preparation.

Figure 1.Differential Recovery of Small RNAs During Total RNA Isolation. Total RNA was isolated from 1x106 HeLa cells using three standard RNA isolation procedures: single phenol/guanidinium extraction (RNAwiz), glass fiber filter (RNAqueous) purification, or double phenol extraction (ToTALLY RNA). Purified RNA samples were analyzed on a 1% denaturing agarose gel and recovery of messenger RNA (-actin, GAPDH), small nuclear RNA (U1 snRNA), and miRNA (miR-16, miR-22) were assessed by Northern blot or solution hybridization with the mirVana miRNA Detection Kit


back to top


Ordering Information
Cat# Product Name Size 1552 mirVana miRNA Detection Kit 100 rxns
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
2. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
3. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
4. Detecting miRNA & siRNA
5. Constructing Directional cDNA Libraries from Limited Amounts of RNA
6. Rapidly Synthesize Large Amounts of Capped RNA
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. New Tools for Small RNA Analysis
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Detecting Attomole Amounts Small RNA

(Date:4/24/2015)... 24, 2015 DryLet, LLC a ... applications such as animal waste reduction, bioremediation, wastewater ... in wastewater treatment plants and restaurant kitchen settings, ... the National Restaurant Association’s NRA Show 2015, in ... of the company will be available to share ...
(Date:4/24/2015)... 2015  Navitas Life Sciences is delighted to announce the ... and Regulatory Services. Shalabh is a proven leader in Life ... of Kinapse, as well as a Strategy consultant at Accenture. ... http://photos.prnewswire.com/prnh/20150423/201261LOGO ... am delighted to welcome Shalabh into this critical role and ...
(Date:4/23/2015)... CA (PRWEB) April 23, 2015 Athena ... personal development of executive women in San Diego’s technology ... the 17th Annual Pinnacle Awards at their Gala on ... Hyatt Hotel. Register now at http://athenasd.org/events/ , ... support the Athena San Diego vision of fostering networking, ...
(Date:4/23/2015)... 2015 Six startups from Russia ... 4-17 as part of the U.S.-Russia Innovation Corridor ... of biotechnology startups to participate in the program ... and the University of Maryland (UMD) signed a ... respective universities in the biomedical industry in 2013. ...
Breaking Biology Technology:DryLet to Showcase Bio-React at the National Restaurant Association’s NRA Show 2015 May 16-19, 2015 in Chicago, Illinois 2Shalabh Kumar Joins Navitas Life Sciences to Lead their Clinical and Regulatory Services Teams 2Athena Pinnacle Awards to Honor Executives, Corporations and Educators Working to Promote Women Leaders 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 3U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 4
... Canopus,BioPharma, Inc. (OTC: CBIA), has confirmed the ... reducing agent and has also demonstrated the,synergistic effect ... and,cetuximab (Erbitux) in two mice lung cancer models., ... stated, "We at,Canopus are encouraged with the discovery ...
... Dominica, June 19 Neuftec,Limited, the proprietor of ... recently issued a claim against Oxonica Energy,Limited, a ... of its,European Patent in the High Court of ... AGM Statement issued by Dr Matthews,(CEO) on 19 ...
... biopharmaceutical industry information on ... ... BioSpace, the world,s,leading online community for the bioscience industry, released ... devices industries in Asia. AsiaBio(TM) features dynamic,informational Hotbed Web pages ...
Cached Biology Technology:CB1400, Patented by Canopus BioPharma, Prevents Tumor Growth and Enhances the Anti-Tumor Effects of Cisplatin and Cetuximab (Erbitux). 2Neuftec Responds to a Fundamental Inaccuracy in Oxonica PLC's Regulatory Announcement 2BioSpace Announces New Online Community for Asia 2
(Date:4/10/2015)... 2015 Research and Markets ( ... "Security Competitive Profiles - NEC" report to their ... NEC will continue to supply a range of IT ... a company focus on the development of a Big ... opportunities in the Asia-Pacific region ...
(Date:4/2/2015)... , April 2, 2015 At its ... Salt Lake City , the American College of ... five new directors to its Board.  Members of the ... ACMG and for forming and advancing its policies and ... genetics profession. "It,s an eventful time in ...
(Date:3/31/2015)... March 31, 2015  Elephant Talk Communications Corp. (NYSE ... provider of Software Defined Network Architecture (ET Software DNA® ... revenue of approximately $20.4 million for the year ended ... million for the year ended December 31, 2013. ... Company had completed its multi-year transition away from its ...
Breaking Biology News(10 mins):NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... has found that species living together are not forced ... challenging a theory that has held since Darwin,s Origin ... the most diverse bird families in the world, the ... of the processes causing species differences to evolve. ...
... , GAINESVILLE, Fla. --- Using the largest dated evolutionary ... suggests how plants developed traits to withstand low temperatures, ... bigger threat than initially thought to plants and global ... the journal Nature and co-authored by University ...
... scientists, chemical engineers and physicists from the University of Pennsylvania ... crystals as a medium for assembling structures. In their ... in the repeating patterns found in liquid crystals, in nanoscale ... pattern out of an even simpler template: a three-dimensional array ...
Cached Biology News:'Be different or die' does not drive evolution 2'Be different or die' does not drive evolution 3Study: Some plants may not adapt quickly to future climate change 2Study: Some plants may not adapt quickly to future climate change 3Penn researchers grow liquid crystal 'flowers' that can be used as lenses 2Penn researchers grow liquid crystal 'flowers' that can be used as lenses 3
From micro to giga sizes, from single prep to high-throughput, Invitrogen's range of plasmid purification kits offers superior performance to match the demands of your downstream application....
Many mammalian cell types and experimental situations create challenges for standard transfection experiments. The ViraPower Lentiviral Expression System overcomes these challenges with efficient...
Many mammalian cell types and experimental situations create challenges for standard transfection experiments. The ViraPower Lentiviral Expression System overcomes these challenges with efficient vir...
Src cDNA Expression Kit (wild type)...
Biology Products: