Navigation Links
Detect Released Oligosaccharides Using the Eagle Eye II,,,Still Video Imaging System


Fast imaging and software flexibility

Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System

Angela R. Downs Deborah A. Greer Stan Ivey
Department of Biology, Delaware State University, Dover, DE

We compared the Eagle Eye II still video system and the Glyko FACE SE2000 gel imager system for imaging Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) gels. The data generated from either system were not significantly different, indicating that successful FACE gel analysis does not require a dedicated single-use device (such as the Glyko SE2000). Consequently, the versatile Eagle Eye II still video system is superior because it can be used for many applications without compromising experimental results.

Glycoprotein carbohydrates play important roles in the biological processes of glycosylated proteins.1 However, analyzing glycan moieties can be an arduous process, given the small recovery amounts, impurity, and low molecular mass of released glycans. In a recent advance, gel electrophoresis has been used to separate fluorophore-labeled oligosaccharides (FACE) released from glycoproteins using endoglycosidases.2

Following electrophoresis, CCD cameras are widely used for all types of fluorescent or colorimetric gel documentation. The Eagle Eye II still video system is designed to visualize, document, and provide analytical tools for any type of DNA or protein gel. In contrast, the Glyko SE2000 gel imager is designed to image only FACE gels.

We compared a FACE gel imaged first using Stratagenes Eagle Eye II still video imaging system (with a SYBR Green band-pass filter), then imaged the gel again using Glykos SE2000 gel imager (Figure 1). We then evaluated the time required
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
3. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
4. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
5. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
6. A New PCR-based Mycoplasma Detection Method
7. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
8. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
9. Improved Vectors for PathDetect Trans-Reporting Systems
10. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
11. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
Post Your Comments:
(Date:1/15/2014)... 2013 was a banner year of continued ... saw continued independent research led by the team at ... $1 million grant from the Susanne Marcus Collins Foundation, ... peer reviewed journal, Amy Grant highlighted Brainwave Optimization® in ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, April ... Location: Warrington Country Club, 1360 Almshouse Road, Warrington, Pa. ... nonprofit organization solely dedicated to finding a cure for hepatitis ... worldwide, will host its annual Crystal Ball on Friday, April ...
(Date:1/14/2014)... As pet owners drew up their lists of New Year,s ... better care of my furry companion." Nowadays there are pet salons, ... cases to take the little canine or feline darlings along wherever ... buy some pricey toys at the pet store. But anyone who ...
(Date:1/14/2014)... iLab Solutions, the leader in core facility management software, ... Strategy. In this role, Michelle will work with current and ... the development of iLab products. Her main goal will be ... the scientific community by offering the most advanced, user-friendly, and ...
Breaking Biology Technology:Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2
... have found a way to turn a precision measurement device ... researchers adapted a device known as an optical frequency comb ... data operations that will be vital for future quantum computers. ... Review Letters and is described in a Viewpoint article ...
... team of nanotechnology researchers from the University of ... microscopy to determine the nanoscale frictional characteristics of ... these very different materials. Friction across these ... layers decreases, all the way down to one ...
... ... or a recipe. It,s a complex system of genes and the molecules that regulate them. ... powerful new way to predict genetic regulatory pathways. , ... Brussels, Belgium (PRWEB) April 1, 2010 -- Simplifying Complexity - New ...
Cached Biology Technology:Nanotechnologists reveal the frictional characteristics of atomically thin sheets 2Simplifying Complexity - New Insights Into How Genomes Work 2Simplifying Complexity - New Insights Into How Genomes Work 3Simplifying Complexity - New Insights Into How Genomes Work 4
(Date:4/17/2014)... ALBUQUERQUE, N.M. A credit-card-sized anthrax detection cartridge developed ... small business makes testing safer, easier, faster and cheaper. ... causes anthrax, is commonly found in soils all over ... illness in both humans and animals. The bacteria can ... to B. anthracis may occur through skin ...
(Date:4/17/2014)... bone marrow need to produce hydrogen sulfide in order ... a new study from the Center for Craniofacial Molecular ... USC. , Professor Songtao Shi, principal investigator on the ... the cells governs the flow of calcium ions. The ... results in osteogenesis, or the creation of new bone ...
(Date:4/17/2014)... and pathogens that devastate honeybees in Europe, Asia and ... do not appear to be impacting native honeybee populations ... researchers., The invasive pests include including Nosema microsporidia ... honeybees appear to be resilient to these invasive pests, ... control pests in Europe, Asia and the United States ...
Breaking Biology News(10 mins):Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3
... PHILADELPHIA - The Advisory Committee on Immunization Practices recommends ... girls, but results of a recent survey showed that ... these recommendations. The survey was published in ... of the American Association for Cancer Research. ...
... - In a little over seven hours, University of Illinois ... than in two years time in a lab. The genetic ... at the U of I. The genetic sequence will allow ... ago genomics was reserved for what Tranel refers to as ...
... Spanish . , Researchers from CIC bioGUNE,s ... a joint research project, published in the prestigious scientific journal ... of pyruvate carboxylase when it is in solution (in the ... plays a fundamental role in the metabolism of fatty acids ...
Cached Biology News:More than half of Texas physicians do not always recommend HPV vaccine to girls 2Understanding how weeds are resistant to herbicides 2Understanding how weeds are resistant to herbicides 3Understanding how weeds are resistant to herbicides 4
Request Info...
Kit contains: Bio-Rad Amplification Reagent, 2x Amplification Diluent, Streptavidin-HRP, Blocking Reagent, and Phosphate Buffered Saline....
... 5 O-rings, 5 barrel liners, 5 white cartridge ... The Helios gene gun is a convenient, hand-held ... into a range of targets in vivo. The ... sweep DNA- or RNA-coated gold microcarriers from the ...
Mouse polyclonal antibody to PRDM2 - PR domain containing 2, with ZNF domain...
Biology Products: