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Designing a Successful qRT-PCR Experiment

will also need appropriate controls.

Master Mix Preparation: A potential source of DNA contamination often arises from prior PCR runs. It is highly recommended that the pre-PCR setup occur at a separate location from post-PCR sample analysis, and that separate pipettes, tips and reaction tubes be used. RT-PCR is highly sensitive to small changes created by pipeting differences and airborne substances. Additionally, Ambion's DNAZap DNA Degradation Solution can be used to decontaminate thermal cyclers, pipettes, and other surfaces prior to RT-PCR.

For a helpful general resource for qRT-PCR approaches and strategies, Ambion recommends the following references: Bustin SA (2000) J Mol Endocrinol 25: 169-93 and Bustin SA (2002) J Mol Endocrinol 29: 23-39.


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Cat# Product Name Size 1745 MessageSensor RT Kit 50 rxn
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