Navigation Links
Designing a Better siRNA


The siRNA design algorithm designed by Cenix BioScience, with whom Ambion has partnered to develop a genome wide siRNA library, represents a major improvement over the design rules first described by Tuschl and colleagues. On average, ~50% of the siRNAs featuring the Tuschl design rules (target sites beginning with AA, 3' UU overhangs for both the sense and antisense siRNA strands, ~50% G/C content; 1-3) provide at least 50% reduction in target gene expression. In contrast, in an initial screen of 79 human genes, 94% of siRNAs designed by Cenix provided greater than 70% reduction in target mRNA levels (Figure 1).

Figure 1.The Effectiveness of Cenix Designed siRNAs. siRNAs targeting 79 human genes were designed using the algorithm developed by Cenix. The top siRNA candidate for each target was prepared and transfected at a 100 nM final concentration. Forty eight hours post-transfection, target gene expression was quantified by real-time RT-PCR. Relative reduction in mRNA expression was measured against cells transfected with a negative control siRNA. Sample size was normalized by measuring 18S rRNA in the various samples using a real-time PCR.


To develop a rational siRNA design algorithm, Cenix and Ambion tested multiple siRNAs targeting hundreds of different human genes that were expressed at detectable levels in several different cell lines. More than 900 siRNAs have been designed and tested to date. The effective and ineffective siRNAs were used to judge the impact of a number of physical characteristics on siRNA potency and specificity, including Tm, nucleotide content of the 3' overhangs, siRNA length, nucleotid
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Designing a Successful qRT-PCR Experiment
2. Designing Controls for siRNA Experiments
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Better RNA Amplification
5. RNA Amplification Just Got Better: MessageAmp II
6. Custom and library siRNA for efficient gene silencing
7. Custom and library siRNA for efficient gene silencing
8. Cancer siRNA Oligo Set Version 1.0
9. Library siRNA
10. Custom siRNA Oligo Synthesis Service
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Designing Better siRNA

(Date:3/26/2015)... the popular news television program that airs in numerous markets ... actor James Earl Jones, has announced that it will address ... Botany is commonly described as the scientific study of plants, ... complicated than that. Scientists and other professionals study a wide ... bacteria known to man to the largest trees that grow ...
(Date:3/25/2015)... Agnition announced today that it will ... airing for the first time at 7:30 a.m. CST ... will explore the extremely important role soil microbes perform ... patented Microbial Catalystâ„¢ technology that enhances soil and plant ... will air again on April 28. , A biochemist ...
(Date:3/25/2015)... YORK , March 25, 2015  S&P ... commenced Factual Stock Report coverage on Abattis ... (OTCQX: ATTBF; CSE: ATT): is a specialty biotechnology ... of cultivating, licensing and marketing proprietary ingredients, bio-similar ... markets in North America . ...
(Date:3/25/2015)... An increase of awareness related to benefits of ... nutrients, regulatory changes and concerns related to consumer safety ... natural astaxanthin over the past few years. Producers of ... to satisfy the rising demand. , The global ... in nutraceuticals, aqua feed, food and beverages and cosmetics) ...
Breaking Biology Technology:Agnition to be Featured In Upcoming Episode of American Farmer on RFD-TV 2S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 5Global Astaxanthin Market to Reach Value of USD 1.1 billion by 2020, Expects Industry Experts in New Report Available at MarketPublishers.com 2
... Technology (NIST) has issued a new ruler, and even for ... records. Designed to be the most accurate commercially available "meter ... standard for X-ray diffractionboasts uncertainties below a femtometer. That,s 0.000 ... a neutron. The new ruler is in the form ...
... , , , ... a prospective registry showed good safety in a broad population of patients ... Genous Bio-engineered R stent for ST-elevation myocardial infarction (STEMI). , , ... of major adverse cardiac events (MACE) was 6.7 percent and the rate ...
... SAN DIEGO, Sept. 22 SoluLinK, Inc., a leader ... Catalyst, making conjugation at least 20 times faster than before ... , , Up to now, researchers, conjugation ... poor yields, poor quality and more labor and time consuming ...
Cached Biology Technology:New NIST nano-ruler sets some very small marks 2OrbusNeich's Genous(TM) Bio-Engineered R Stent(TM) is Safe in a Broad Population of Patients with Primary PCI for STEMI 2OrbusNeich's Genous(TM) Bio-Engineered R Stent(TM) is Safe in a Broad Population of Patients with Primary PCI for STEMI 3SoluLinK Introduces Product that Links All Biomolecules Greater Than 20 Times Faster with 100% Yields 2
(Date:3/11/2015)... , Mar. 11, 2015 Research and Markets ... the "Access Control Market by Product, Application & ... report to their offering. , This ... to reach $10.4 billion by 2020, with an estimated ... report covers the products types such as contact cards ...
(Date:3/10/2015)... BOULDER, Colo. , March 10, 2015 ... Space Flight "Personalized Medicine in Human ... Ph.D. and Thomas J. Goodwin , Ph.D. was ... articles of the past two years. Specifically, ... three most downloaded scientific papers published in 2013 and ...
(Date:3/5/2015)... March 5, 2015 In Brazil ... companies already using these solutions and 42 percent planning to ... latter, approximately 25 percent of the companies will be investing ... the majority of companies in the region are currently opting ... hybrid model will command significant attention in the coming years. ...
Breaking Biology News(10 mins):Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2"Personalized Medicine in Human Space Flight" Listed Among Most Influential Papers of 2013 and 2014 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 3Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 4
... in their genes" is a common refrain from scientists when ... and beyond. Up until now, research has focused on ... levels of HDL ("good") cholesterol. But researchers at Albert ... of Psychology of Yeshiva University have found that personality ...
... Preventice, Inc. , a developer of mobile health ... it is the latest to join medical device ... payors that are integrating with Qualcomm Life Inc., ... their 2net™ Ecosystem. (Logo: http://photos.prnewswire.com/prnh/20120417/CG89172LOGO ...
... Diego have discovered that a small dose of a commonly ... affects their ability to recruit their nestmates to otherwise good ... this week,s issue of the Journal of Experimental Biology ... bee-pollinated crops and shed light on one of the main ...
Cached Biology News:'Personality genes' may help account for longevity 2Preventice Expands Options for Wireless Patient Monitoring with Qualcomm Life 2Commonly used pesticide turns honey bees into 'picky eaters' 2
ANTI PIG LDH H4...
RAT ANTI MOUSE F4/80 ANTIGEN:BIOTIN Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice...
... in refrigerators, freezers, or liquid nitrogen. Conveniently sized to ... color coding to project, user, lab, etc. Holes in ... air circulation. Supplied with 10 x 10 (11.6 mm ... dimensions: 13 x 13 x 5.1 cm H (5 ...
Sterile glass 5 mm beads that make spreading bacterial and yeast cultures easy. Can be washed, autoclaved and reused....
Biology Products: