Navigation Links
Designing a Better siRNA


The siRNA design algorithm designed by Cenix BioScience, with whom Ambion has partnered to develop a genome wide siRNA library, represents a major improvement over the design rules first described by Tuschl and colleagues. On average, ~50% of the siRNAs featuring the Tuschl design rules (target sites beginning with AA, 3' UU overhangs for both the sense and antisense siRNA strands, ~50% G/C content; 1-3) provide at least 50% reduction in target gene expression. In contrast, in an initial screen of 79 human genes, 94% of siRNAs designed by Cenix provided greater than 70% reduction in target mRNA levels (Figure 1).

Figure 1.The Effectiveness of Cenix Designed siRNAs. siRNAs targeting 79 human genes were designed using the algorithm developed by Cenix. The top siRNA candidate for each target was prepared and transfected at a 100 nM final concentration. Forty eight hours post-transfection, target gene expression was quantified by real-time RT-PCR. Relative reduction in mRNA expression was measured against cells transfected with a negative control siRNA. Sample size was normalized by measuring 18S rRNA in the various samples using a real-time PCR.


To develop a rational siRNA design algorithm, Cenix and Ambion tested multiple siRNAs targeting hundreds of different human genes that were expressed at detectable levels in several different cell lines. More than 900 siRNAs have been designed and tested to date. The effective and ineffective siRNAs were used to judge the impact of a number of physical characteristics on siRNA potency and specificity, including Tm, nucleotide content of the 3' overhangs, siRNA length, nucleotid
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Designing a Successful qRT-PCR Experiment
2. Designing Controls for siRNA Experiments
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Better RNA Amplification
5. RNA Amplification Just Got Better: MessageAmp II
6. Custom and library siRNA for efficient gene silencing
7. Custom and library siRNA for efficient gene silencing
8. Cancer siRNA Oligo Set Version 1.0
9. Library siRNA
10. Custom siRNA Oligo Synthesis Service
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Designing Better siRNA

(Date:7/10/2014)... 10, 2014 Women in the ... to an additional family planning option. Sayana® Press ... at all levels of the health system and ... a widely used contraceptive—Pfizer’s Depo-Provera® (depot medroxyprogesterone acetate)—with ... is the first of four African countries expected ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Unraveling life’s ... together. The Archer Family purchased a DNA ... DNA, but ended up with a lifetime of memories, ... within the family members’ DNA genuinely brought the family ... to search deeper into genetic history and prior to ...
(Date:7/10/2014)... , July 10, 2014  Franciscan ... use of capnography for respiratory monitoring outside ... of healthcare leaders in embracing state-of-the-art patient ... effectively patients are breathing and can alert ... By measuring the amount of carbon dioxide ...
(Date:7/10/2014)... July 10, 2014   Ceres, Inc . (Nasdaq: ... company, announced today financial results for the three months ... its business. Ceres reported that the company ... performance this growing season in Brazil, which concluded in ... company,s sorghum evaluation areas for part of the growing ...
Breaking Biology Technology:Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9
... Mass. , July 14 Sermo ( http://sermo.com/client ... Client Center. Based on four years of client feedback and ... meets demands for enhanced navigation, mining and monitoring of organic ... million comments and nearly 4 million votes, Sermo is rich ...
... , AKRON, Ohio , July 13 State and Federal Communications, ... report on how states are responding to the Supreme Court,s landmark decision in ... , , To ... "Citizens United Update" in the lower left of the home page. The report is ...
... of Technology announces a new type of nanopore devices that ... example to read off their sequence. In a paper entitled ... Letters ), they report a novel technique to fabricate tiny ... a thickness of only 1 atom) and they managed to ...
Cached Biology Technology:Sermo Announces New Client Center with Advanced Mining and Monitoring of Organic Physician Dialog 2Sermo Announces New Client Center with Advanced Mining and Monitoring of Organic Physician Dialog 3Report Details How States Respond to Citizens United Decision 2DNA through graphene nanopores 2
(Date:7/10/2014)... CORAL SPRINGS, Florida , July 1, 2014 ... smart wallets pave way for convenience and improved security: NXT-ID, ... Google Inc. (NASDAQ: GOOG ), Apple Inc. (NASDAQ: ... ) and MasterCard Corporation (NYSE: MA) NXT-ID, Inc., (OTCQB: ... market, is pleased to announce that the second series of ...
(Date:7/10/2014)... -- According to the new market research ... Material (Optical Prism, Pizeoelectric, Capacitive, and Adhesives), Application (Mobile ... Finance), and Geography - Global Trends & Forecasts to ... Sensors Market is projected to cross $14.35 Billion by ... 2014 to 2020. Browse more than 78 ...
(Date:7/10/2014)... Sweden , July 8, 2014 ... has won a new design win (DW). An Asian OEM ... date for mass production start in August 2014.   ... Asian smartphone OEM, which has a planned date for start ... an initial ramp order of SEK 5M, for delivery in ...
Breaking Biology News(10 mins):Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5Fingerprint Sensors Market worth $14.35 Billion by 2020 2Fingerprint Sensors Market worth $14.35 Billion by 2020 3FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2
... do skin cells know when to proliferate and at ... the Journal of Cell Biology , Nguan Soon ... protein called PPARβ/δ to make sure overlying epithelial cells ... between different cell types are critical to maintain the ...
... called quantum tunneling, may aid the quest for accurate, low-cost ... Nanotechnology Letters by Stuart Lindsay and his collaborators at ... a particle, say an electron, can cross a barrier, when, ... to do so. Unraveling the DNA sequences of the ...
... in Technology and Innovation in Global BiometricsGAITHERSBURG, Md., ... LMT ) has been named the Biometrics Systems ... Sullivan, recognizing the Corporation,s leadership in developing innovative ... Frost and Sullivan based the award on a ...
Cached Biology News:Time (and PPAR-beta/delta) heals all wounds 2Time (and PPAR-beta/delta) heals all wounds 3Covering the bases: Quantum effect may hold promise for low-cost DNA sequencing, sensor applications 2Covering the bases: Quantum effect may hold promise for low-cost DNA sequencing, sensor applications 3Covering the bases: Quantum effect may hold promise for low-cost DNA sequencing, sensor applications 4Lockheed Martin Named Biometrics 'Company of the Year' by Frost and Sullivan 2
Applications: Epitope mapping, Mapping protein-protein contacts, Identification of enzyme substrates/inhibitors, Identification of receptor agonists/antagonists...
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Request Info...
QML offers standard and customized paraffin embedded tissue blocks....
Biology Products: