Navigation Links
Designing a Better siRNA


The siRNA design algorithm designed by Cenix BioScience, with whom Ambion has partnered to develop a genome wide siRNA library, represents a major improvement over the design rules first described by Tuschl and colleagues. On average, ~50% of the siRNAs featuring the Tuschl design rules (target sites beginning with AA, 3' UU overhangs for both the sense and antisense siRNA strands, ~50% G/C content; 1-3) provide at least 50% reduction in target gene expression. In contrast, in an initial screen of 79 human genes, 94% of siRNAs designed by Cenix provided greater than 70% reduction in target mRNA levels (Figure 1).

Figure 1.The Effectiveness of Cenix Designed siRNAs. siRNAs targeting 79 human genes were designed using the algorithm developed by Cenix. The top siRNA candidate for each target was prepared and transfected at a 100 nM final concentration. Forty eight hours post-transfection, target gene expression was quantified by real-time RT-PCR. Relative reduction in mRNA expression was measured against cells transfected with a negative control siRNA. Sample size was normalized by measuring 18S rRNA in the various samples using a real-time PCR.


To develop a rational siRNA design algorithm, Cenix and Ambion tested multiple siRNAs targeting hundreds of different human genes that were expressed at detectable levels in several different cell lines. More than 900 siRNAs have been designed and tested to date. The effective and ineffective siRNAs were used to judge the impact of a number of physical characteristics on siRNA potency and specificity, including Tm, nucleotide content of the 3' overhangs, siRNA length, nucleotid
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Designing a Successful qRT-PCR Experiment
2. Designing Controls for siRNA Experiments
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Better RNA Amplification
5. RNA Amplification Just Got Better: MessageAmp II
6. Custom and library siRNA for efficient gene silencing
7. Custom and library siRNA for efficient gene silencing
8. Cancer siRNA Oligo Set Version 1.0
9. Library siRNA
10. Custom siRNA Oligo Synthesis Service
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Designing Better siRNA

(Date:5/23/2013)... 2013 Ralph Huber, President of PHI ... management products and emergency survival kits would be on ... made at the quarterly executive board meeting on May 22nd. ... silence for the Oklahoma tornado victims and used the opportunity ... their emergency kits is paramount. “When disaster strikes,” he ...
(Date:5/22/2013)... Cambridge Semantics was one of just ... Day” on Capitol Hill, hosted by The Data ... others to showcase how innovative data management applications can address ... (DATA Act). , Originally introduced in 2011 by Committee ... Mark Warner (D-VA ), the DATA Act is ...
(Date:5/22/2013)... CANTON, Mass. and NEW ORLEANS ... commercial leader in the field of regenerative medicine, presented ... expert economists from Analysis Group (a leading health economics ... that diabetic foot ulcers (DFU) and venous leg ulcers ... The results of this research, which reveal annual ...
(Date:5/22/2013)... Earth, MN (PRWEB) May 22, 2013 ... Royal Medical Supplies Pty Ltd are pleased to ... Medical is the Australian and New Zealand distributor of ... Motorsport V8 Supercar Team participated in the Austin 400 ... has entered into an agreement that involves each car ...
Breaking Biology Technology:PHI Emergency Management on Announces Father's Day Specials All Emergency Survival Kits 2Cambridge Semantics Underscores Need for Smart Data during “Data Demonstration Day” on Capitol Hill 2Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 2Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 3Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 4Australian Distributor of Express Diagnostics Signs Sponsorship Agreement with V8 Supercar Team 2
... ... Research Adopts the Genedata Screener® 7.0 Data Management Platform for High Throughput and High ... ... leading provider of advanced software solutions for drug discovery and life science research, and ...
... SAN DIEGO, Dec. 9 BioMed Realty Trust, Inc. (NYSE: ... results of the previously announced cash tender offer by its ... its outstanding 4.50% Exchangeable Senior Notes due 2026 (the "Notes"). ... December 8, 2009, the scheduled expiration date of the tender ...
... , MILFORD, Mass., Dec. 9 Waters Corporation (NYSE: ... first units of its ground-breaking SYNAPT(TM) G2 Mass Spectrometer, first ... Spectrometry (ASMS) annual conference in Philadelphia in June. , The ... North America and Asia and for global pharmaceutical companies and ...
Cached Biology Technology:MEDINA Scientists Select Genedata Screener for Lead Discovery from Natural Products 2MEDINA Scientists Select Genedata Screener for Lead Discovery from Natural Products 3MEDINA Scientists Select Genedata Screener for Lead Discovery from Natural Products 4BioMed Realty Announces Results of Tender Offer for its Exchangeable Notes 2BioMed Realty Announces Results of Tender Offer for its Exchangeable Notes 3Waters Ships First Units of SYNAPT G2 Mass Spectrometer 2
(Date:5/22/2013)... for prostate cancer could become as easy for men ... UC Irvine research published today in the Journal ... than a decade of work, UC Irvine chemists have ... for prostate cancer in urine, meaning that the disease ... at dramatically lower cost. The same technology could potentially ...
(Date:5/21/2013)... device attached to a whale entangled in fishing gear, ... changed a whale,s diving and swimming behavior. The monitoring ... and migrate, depletes their energy as they drag gear ... slow death. , The scientists in this entanglement response ... two-year-old female North Atlantic right whale called Eg 3911. ...
(Date:5/21/2013)... of pharmaceuticals can both prevent and treat Alzheimer,s Disease ... ligands," are currently used for certain types of neuroimaging. ... in young adult mice when pathology was at an ... quite severe," said lead researcher Christian Pike of the ... of pathology and improved behavior at both ages." , ...
Breaking Biology News(10 mins):UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3Study reveals how fishing gear can cause slow death of whales 2Study reveals how fishing gear can cause slow death of whales 3Drugs found to both prevent and treat Alzheimer's disease in mice 2
... studying a habituated population of pied babblers (Turdoides bicolor) in ... in which parent birds can extend the period of their ... of the University of Cambridge and Amanda Ridley of the ... issue of Current Biology, published by Cell Press. , ...
... activate certain sets of neurons and effectively substitute activation ... have been able to identify neurons in the fruit ... stimuli during so-called associative learning. The findings, which advance ... learn to associate cues with positive and negative experiences, ...
... Researchers from the Swiss Institute for Experimental Cancer Research ... embryonic development is responsible for two seemingly contradictory activities ... in the mother's uterus, and later providing cues to ... time is ripe. , The development of an embryo ...
Cached Biology News:Researchers identify neurons that assign value during learning 2Who gives stem cells their marching orders? 2
... Adept CE 4100 high pressure pump ... precision, with software drive compensation, reducing pulsing ... are selectable in 0.001mL steps. , The ... and bio-compatible., Pumps may be programmed from ...
High affinity antibodies recognizes HSP70 protein from human cardiac tissue. Could be used for Westerb blotting application, ELISA....
Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm 2 or 5-10 μl/cm 2 . Optimal concentration depends on cell type as well as the application or research objectives. Type B...
Monoclonal antibodies conjugated with rhodamine...
Biology Products: