Navigation Links
Denaturing HPLC (DHPLC)

umn.

There are columns on the market for performing fragment analysis, they include the DNASep Column, the Helix DVB column and the Zorbax Eclipse dsDNA Analysis Column.

The ion-pairing agent used with either column is triethylammonium acetate (TEAA), which mediates binding of DNA to the stationary phase. Acetonitrile is used as an organic agent to achieve subsequent separation of the DNA from the column.


Typical Method:

Using the system described above, the following reagents and column can be used
Standards of ds DNA
• Deonised water
• HPLC grade acetonitrile
• Buffer A, comprising of 100 mM TEAA to pH 7.0, 0.1 mM EDTA,
• Buffer B, comprising of 100 mM TEAA to pH 7.0, 0.1mM EDTA and 25 %v/v acetonitrile
• Helix DVB column, 3mm ID x 50 mm length


Set the detection wavelength to 260 nm.

A typical binary gradient cycle is:
A flow rate of 0.45 ml/minute
Percentage of buffer A, when time is 0.00 minute – 55%
Percentage of buffer A, when time is 0.30 minutes – 50%
Percentage of buffer A, when time is 6.00 minutes – 32%
Percentage of buffer A, when time is 7.00 minutes – 32%
Percentage of buffer A, when time is 7.05 minutes – 55%

Samples and standards may need to be diluted with deionised water.

Run the standard to ensure that the required peaks are well resolved. The column may need to be washed, or a wait may be required for the column to equilibrate to the required temperature.

Between changes in temperature, always ensure that the column has had sufficient time to equilibrate to the required temperature.

Optimisation of the denaturing temperature can be performed by trial or error. Here injection
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
4. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
5. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
6. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
8. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Denaturing HPLC DHPLC

(Date:1/22/2015)... Cambridge Semantics, the leading provider of smart data ... was a record-breaking year across the board for the company. ... Data Platform and our Smart Data solutions, our customers were ... which led to record growth for the company in 2014,” ...
(Date:1/22/2015)... Boston, MA (PRWEB) January 22, 2015 ... systems, is pleased to announce an exciting product from a ... of motion analysis solutions over the past 15 years, with ... industries. Most recently, their 3D IMU system (inertial measurement unit), ...
(Date:1/22/2015)... , 22 de enero de 2015  El Dr. ... su llamada a nominaciones para 2015. Este prestigioso premio ... o tiene el potencial para hacer, contribuciones destacadas para ... aceptarán hasta el 15 de marzo de 2015 a ...
(Date:12/25/2014)... 25, 2014 The report “Service Quality ... Type & Provider Type - Global Advancements, Worldwide Forecasts ... an in-depth analysis and forecasting of revenues. , ... 221 pages and in-depth TOC on “Service Quality Management ...
Breaking Biology Technology:Cambridge Semantics Announces Record Results for 2014 23D Motion Capture Just Got Easy 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4
... Scarless Hair Transplantation,(International) Medical Chain Institute today announced ... the prestigious Chang,an Avenue West,Beijing, China. The ... and plastics institution for hair transplants in China. ... area of 1,000 square meters, the Hospital is ...
... April 22 Three attorneys who previously worked at ... firm,s Atlanta office. The attorneys include Mark S. Lange, ... department and Larry A. Slovensky, a partner ... welcome good lawyers back into the fold," said MLA ...
... DIEGO, CA, April 23, 2009 Senomyx, Inc. (Nasdaq: ... results on Thursday, May 7, 2009 before the opening of ... a conference call with a live Internet broadcast that will ... quarter results on the same day at 11:00 a.m. Eastern ...
Cached Biology Technology:Lotus Scarless Hair Transplantation (International) Medical Chain Institute Opens New Center on Beijing's Prestigious Chang'An Avenue 2Three Attorneys Rejoin McKenna Long & Aldridge 2SENOMYX, INC. FIRST QUARTER 2009 EARNINGS CONFERENCE CALL SCHEDULED FOR MAY 7, 2009 2
(Date:12/17/2014)... , Dec. 16, 2014  Automation is fundamentally transforming the ... at international borders. Over the past decade, ePassports, biometric ... to self process through border control via eGates and ... of airports, seaports, and land borders across the globe. ...
(Date:12/11/2014)... Markets , http://photos.prnewswire.com/prnh/20130307/600769 ... addition of the "Biometrics Market in India 2015-2019" ... major trend emerging in this market is the adoption ... than one characteristic of an individual for verification and ...
(Date:12/10/2014)... , Dec. 8, 2014 You,ve been here before: you ... remember your password, site key or the answer to your secret ... Today, Hoyos Labs , a digital infrastructure ... an end to the frustration that comes with usernames, passwords and ...
Breaking Biology News(10 mins):Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... AMHERST, Mass. Military uniforms of the future may ... thanks to research by teams at the University of ... a nanotube-based fabric that repels chemical and biological agents. ... Watkins, collaborating with team leader Francesco Fornasiero of Lawrence ...
... trees is so finely-tuned that predicted increases in drought ... in many species. A recent paper co-authored by George ... finds that those systems in plants around the globe ... making forest ecosystems vulnerable to increasing environmental stress., In ...
... molecules are said to be hydrophobic. The hydration ... is important for many biological processes: protein folding, membrane ... of action potentials across membranes. It is involved as ... the fact that you can get rid of fat ...
Cached Biology News:Developing 'second skin' military fabric to repel chemical and biological agents 2Developing 'second skin' military fabric to repel chemical and biological agents 3Increasing drought stress challenges vulnerable hydraulic system of plants, GW professor finds 2Illuminating the no-man's land of waters' surface 2
For microscopy...
... An easy-to-use solution that enables fully automated ... 400 Hybridization Station has been designed to ... the throughput necessary to perform a wide ... performed in the range 485C; denaturations of ...
... microarray slide processing offering reproducibility, reliability ... to miniaturization, automation and massive parallel ... tool with many applications in both ... range from gene expression profiling, gene ...
... Eppendorf Thermomixer R offers maximum application ... shaking, heating, and cooling. Its active ... application and temperature control ranges. Five ... 24 micro test tubes, are available ...
Biology Products: