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Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

e of use of biolistic delivery, as well as the ability of biolistic delivery to generate specific long-lived immune responses. Future work will further elucidate the diversity of the immune responses that are generated following DNA delivery of the pCMV-S plasmid using the Helios gene gun system. In particular, future work will investigate whether pCMV-S vaccination by gene gun is able to stimulate cell-mediated immune responses in addition to the humoral responses demonstrated in our work to date. The IPQSLDSWWTSL peptide containing the dodecameric class I Ld-restricted epitope of the hepatitis B envelope will be used in cytotoxic T cell assays and tetramer analysis. This not only allows the generation of standardized assays, but also provides a control against which other vaccines can be tested.


Conclusion

Delivery of the pCMV-S plasmid using the Helios gene gun produced titers of antibodies against HBsAg that were up to 1,000 times greater than those observed with intramuscular vaccination. Antibody responses following gene gun delivery of pCMV-S were long-lived and did not decline for at least 12 weeks after initial vaccination. The Helios gene gun system represents a reliable and reproducible method of DNA vaccination, which, when used to deliver the pCMV-S plasmid, results in sustained high antibody titers.


References
Chen CH et al., Gene gun-mediated DNA vaccination induces antitumor immunity against human papillomavirus type 16 E7-expressing murine tumor metastases in the liver and lungs, Gene Ther 6, 19721981 (1999)

Chen Z et al., Cross-protection against a lethal influenza virus infection by DNA vaccine to neuraminidase, Vaccine 18, 3
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