Navigation Links
Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

ults
Intramuscular Vaccination
None of the six mice that received a single intramuscular vaccination developed an antibody response to the HBsAg. However, a response was obtained in four of six mice (detected at a 1:10 antiserum dilution) following two intramuscular vaccinations (Figure 1).

Gene Gun Vaccination
Four of six mice that received a single Helios gene gun vaccination developed an antibody response to the HBsAg (detected at a 1:10 antiserum dilution), with one of these responses measured at a dilution of 1:100. All six mice that received two gene gun vaccinations demonstrated antibody responses, detectable at an antiserum dilution of 1:10,000 for two mice, 1:1,000 dilution for three mice, and 1:100 dilution for one mouse, respectively (Figure 2).

Antibody Response by Subclass
The relative binding activity of the anti-IgG1 antibody was greater than that of the anti-IgG2a antibody. After taking this into account, we found that intramuscular vaccination with pCMV-S resulted in a predominantly IgG2a response, while Helios gene gun vaccination resulted in a predominantly IgG1 response (Figure 3).

Duration of Antibody Response Antisera obtained at weeks 4, 6, 9, and 12 after gene gun vaccination were all assessed by ELISA for the presence of antibodies to HBsAg. At all time points there was a significantly higher antibody titer compared to unimmunized control sera at a dilution of 1:1,000 (Figure 4). Antibody titers for the week 12 antisera are shown in Figure 5.


Discussion
Delivery of the pCMV-S p
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
2. High-Titer Retroviral Vectors for Gene Delivery
3. A New Way to Assess siRNA Delivery Efficiency
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. Enhanced siRNA Delivery and Long-term Gene Silencing
6. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
7. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
8. Oil-Free PCR Using the Hot Top Assembly
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
11. Using XL1-Red Mutator Strain to Generate Esterase Variants
Post Your Comments:
(Date:7/25/2014)... (PRWEB) July 25, 2014 At the ... has granted a 60-day extension for the public comment ... Special Rule for Model Aircraft established by Congress as ... 2012. The 60-day extension establishes the new deadline for ... States Department of Transportation/FAA notice published in the Federal ...
(Date:7/24/2014)... 24, 2014  Now available is a stem cell ... Regenerative Therapies , located in Guadalajara, Mexico ... people a new health option: stem cell therapies ... countless patients suffer from joint and muscle injuries due ... tears, torn rotator cuff, tennis elbow, and knee injuries. ...
(Date:7/24/2014)... have yet to materialise. Yet, scientists are making ... computers faster. One such approach relies on quantum ... applying an electric field. A new study demonstrates ... quantum dots (TQDs) with electrical impulses can help ... should TQDs be used as quantum information units, ...
(Date:7/24/2014)... July 24, 2014 Your cell ... in modeling lithium-ion battery storage capacity. , New ... a theoretical model created at Lawrence Livermore ... that predicts how carbon components will perform as ... emphasizes the urgent need for higher-performance batteries. Several ...
Breaking Biology Technology:FAA Grants Comment Extension at the Request of AMA 2Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3Getting More Life out of Lithium-ion Batteries 2
... Remove >90% of mammalian RNA from , ... Simple procedure takes less than 2 hours , ... Seamless integration with , MICROB Express ... microarray expression analysis with , bacterial RNA isolated from host cells ...
... , , ... methods to isolate high quality RNA from a large number of ... the RNAqueous-96 , Kit . This kit, which ... of intact RNA in a 96-well plate format. An optional on-the-filter , ...
... Amplification Kits-- Now Available with Cy Dyes , ... Extremely sensitive , ArrayScript Reverse , ... yields , ... 4000-fold amplification of mRNA , Optimized buffers--high dye coupling efficiency , ...
Cached Biology Technology:Bacterial RNA Isolation From Infected Eukaryotic Hosts 2Bacterial RNA Isolation From Infected Eukaryotic Hosts 3Bacterial RNA Isolation From Infected Eukaryotic Hosts 4Bacterial RNA Isolation From Infected Eukaryotic Hosts 5Bacterial RNA Isolation From Infected Eukaryotic Hosts 6Automating RNA Isolation 2Automating RNA Isolation 3Automating RNA Isolation 4Automating RNA Isolation 5aRNA Synthesis and Labeling for Array Analysis 2aRNA Synthesis and Labeling for Array Analysis 3aRNA Synthesis and Labeling for Array Analysis 4aRNA Synthesis and Labeling for Array Analysis 5
(Date:7/24/2014)... Angeles, where electric cars ply silent freeways, solar panels ... beneath the earth, from howling winds and from the ... that it is technically and economically feasible to convert ... renewable energy. Published in Energy , the plan ... energy supply in California that could create tens of ...
(Date:7/24/2014)... 24, 2014 Some sticky research out of York ... a certain species of toxic grass fungus: moose saliva ... Biology Letters , "Ungulate saliva inhibits a grassendophyte ... to red fescue grass (which hosts a fungus called ... slower fungus growth and less toxicity. , "Plants have ...
(Date:7/24/2014)... of researchers has developed a new nanoscale agent for ... for assessing the function and properties of the GI ... and treatment of gut diseases. , Illnesses ... and inflammatory bowel disease all occur in the intestine ... with diseases such as diabetes and Parkinson,s. , ...
Breaking Biology News(10 mins):Stanford study shows how to power California with wind, water and sun 2Stanford study shows how to power California with wind, water and sun 3Stanford study shows how to power California with wind, water and sun 4Moose drool inhibits growth of toxic fungus: York U research 2New imaging agent provides better picture of the gut 2
... Inc., today announced that its 12,200 Online Books ... of HINARI, AGORA and OARE, benefitting research and ... including Malawi, Cambodia, and Bolivia. Research4Life provides ... low cost access to peer-reviewed online content from ...
... /PRNewswire-iReach/ -- Successful businesses stay connected to the ... with industry leaders at global biotech conferences with ... go to learn about new innovations, how to ... and risks while overcoming regulatory and technological hurdles. ...
... by English naturalist Charles Darwin on the Galapagos Islands, ... as isolation on a particular island, can lead to ... natural selection. Natural selection is a process whereby ... successive generations, while unfavorable heritable traits become less common. ...
Cached Biology News:Over 12,000 Wiley Online Books made available in developing countries via Research4Life 2Early Bird Conference Registrations Expiring This Friday 2Early Bird Conference Registrations Expiring This Friday 3Early Bird Conference Registrations Expiring This Friday 4LSU professor discovers how new corals species form in the ocean 2LSU professor discovers how new corals species form in the ocean 3
Request Info...
See product name for description....
... Amino acid mixture excluding l-[U-14C]methionine, ... containing 2% ethanol, sterilized. > ... mCi/mg atom carbon. Mixture of ... Ile, Leu, Lys, Phe, Pro, Ser, ...
Request Info...
Biology Products: