Navigation Links
Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

e digestion using BamHI and analysis by agarose gel electrophoresis.

Preparation of DNA-Coated Gold Microcarriers
On the day prior to vaccination, pCMV-S plasmid DNA was precipitated onto gold microcarriers as detailed in the Helios gene gun system instruction manual. Briefly, 8.3 mg of 1 m gold microcarriers was resuspended by sonication in 100 l of 0.05 M spermidine. Eighteen micrograms (18 g) of DNA at a concentration of 1 mg/ml in endotoxin-free water was then added and sonicated; 100 l of 1 M CaCl2 was then added dropwise. This gold-DNA mixture was allowed to stand for 10 min before being washed 3 times in 250 l of 100% ethanol. After the final wash, the pellet was resuspended in 200 l of 0.025 mg/ml polyvinylpyrrolidone (PVP) in 100% ethanol, transferred to a 15 ml tube, and made up to 1 ml with PVP/ethanol. This resulted in a microcarrier loading quantity (MLQ) of 0.5 mg of gold per shot and a DNA loading ratio (DLR) of 2 g/mg gold, which results in the delivery of 1 g of DNA per shot.

Loading DNA/Microcarrier Suspension into GoldCoat Tubing
This was performed as detailed in the Helios gene gun system instruction manual, with 1 ml of DNA/microcarrier suspension being used to produce 17 coated 0.5-inch cartridges, which were then stored overnight at 4C with desiccant prior to use.

Vaccination of Mice
Female Balb/c strain mice aged 68 weeks were obtained from Charles River, UK, and housed at the Biomedical Services Unit, University of Nottingham, UK.

For gene gun delivery, the abdominal fur of each mouse was removed with electric clippers prior to each vaccination. The barrel liner of the Helios gene gun was then held directly against
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
2. High-Titer Retroviral Vectors for Gene Delivery
3. A New Way to Assess siRNA Delivery Efficiency
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. Enhanced siRNA Delivery and Long-term Gene Silencing
6. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
7. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
8. Oil-Free PCR Using the Hot Top Assembly
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
11. Using XL1-Red Mutator Strain to Generate Esterase Variants
Post Your Comments:
(Date:8/28/2014)... August 28, 2014 PrimeSource Building ... materials, selected SoundConnect to support their ... based audio and web conferencing platform GlobalMeet powered ... PrimeSource’s employees’ needs. SoundConnect delivers award winning ... PrimeSource Building Products will be utilizing GlobalMeet ...
(Date:8/27/2014)... (PRWEB) August 27, 2014 Varian Medical ... expand its current Salt Lake City manufacturing facility and ... of Utah to ultimately create 1,000 new full time ... , “Utah is home to hardworking people with ... be credited with Varian’s success in the manufacturing of ...
(Date:8/27/2014)... (PRWEB) August 27, 2014 In ... through Lawrence Livermore National Security LLC ... develop and deliver a state-of-the-art laser system for ... (ELI Beamlines), under construction in the Czech Republic. ... the laser system, called the " High repetition-rate ...
(Date:8/27/2014)... of nature,s mysteries is how plants survive impact ... the sun,s rays, while using this energy for ... in the plant,s blades quickly dissipate the energy ... quakes. Researchers at DTU Physics have now managed ... algae and bacteria contain light-absorbing proteins which play ...
Breaking Biology Technology:PrimeSource Chooses SoundConnect as Collaboration Provider 2Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 2Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 3LLNL Synchs up with ELI Beamlines on Timing System 2LLNL Synchs up with ELI Beamlines on Timing System 3DTU researchers film protein quake for the first time 2
... right size and existing in the limbo between a solid ... improving the efficiency of chemical catalysts that can create better ... in this transitional state, a catalyst can achieve its utmost ... temperature, according to a pair of Duke University researchers. A ...
... At the heart of the method is a so-called ... channel in a semiconductor circuit. The scientists created a ... of electrons in the semiconductor. The key is that ... the channel, making possible extremely high-precision measurements of the ...
... Transgenomic, Inc. (OTC Bulletin Board: TBIO ... the company as Medical Director for Laboratory Services.  In ... for leading the strategic vision and managing the day-to-day ... Dr. Johnson brings to Transgenomic nearly 20 years ...
Cached Biology Technology:Smaller is better in the viscous zone 2Dr. Eric Johnson Joins Transgenomic as Medical Director for Laboratory Services 2Dr. Eric Johnson Joins Transgenomic as Medical Director for Laboratory Services 3Dr. Eric Johnson Joins Transgenomic as Medical Director for Laboratory Services 4
(Date:8/28/2014)... FASEB MARC (Maximizing Access to Research Careers) ... the Genetics Society of America,s 27th Annual Mouse ... 3, 2014 in Pacific Grove, California. These ... students, post doctorates and scientists from underrepresented groups ... and to encourage the participation of young scientists ...
(Date:8/28/2014)... that could positively impact the global battle ... thanks to researchers in Penn State,s College ... made from the seaweed-derived food ingredient carrageenan ... provides a woman-initiated, drug-delivery vehicle that can ... infections during unprotected heterosexual intercourse, the researchers ...
(Date:8/28/2014)... the most commonly diagnosed learning disability in the ... occurs when the regions of the brain that ... use of non-invasive functional neuroimaging tools has helped ... However, most prior work has focused on only ... gap in our understanding of how multiple brain ...
Breaking Biology News(10 mins):Sensory-tested drug-delivery vehicle could limit spread of HIV, AIDS 2Sensory-tested drug-delivery vehicle could limit spread of HIV, AIDS 3Dyslexic readers have disrupted network connections in the brain 2
... caught male topi antelopes in the act of faking fear in ... chances of having sex. The male antelopes, observed in southwest ... when females in heat are in their territories. When the females ... for some males to fit in a quick mating opportunity. ...
... , May 24 BIO-key International, Inc. (OTC Bulletin ... that Evidian has integrated BIO-key,s biometric software into its Enterprise Single ... solution at two major pharmaceutical companies. , ... (Logo: http://www.newscom.com/cgi-bin/prnh/20050509/BIOKEYLOGO ) , ...
... Neurone Disease Association is funding its first ever stem ... this fatal, neurological condition. Pulling together world-class ... Prof Sir Ian Wilmut from the University of Edinburgh; ... Tom Maniatis of Columbia University New York; the Association,s ...
Cached Biology News:Male antelopes trick females into extra sex opportunities 2Male antelopes trick females into extra sex opportunities 3BIO-key(R) Biometric Identification Now Incorporated Into Evidian Enterprise Single Sign-On Solution 2BIO-key(R) Biometric Identification Now Incorporated Into Evidian Enterprise Single Sign-On Solution 3BIO-key(R) Biometric Identification Now Incorporated Into Evidian Enterprise Single Sign-On Solution 4Harnessing the power of stem cells to unlock the secrets of motor neuron disease 2Harnessing the power of stem cells to unlock the secrets of motor neuron disease 3Harnessing the power of stem cells to unlock the secrets of motor neuron disease 4
...
Human sIL-2R/CD25 ELI-PAIR Kit...
STX18 Immunogen: STX18 (NP_058626, 101 a.a. ~ 200 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Lipid Phosphate Phosphohydrolase 2...
Biology Products: