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Delivering siRNAs to Difficult Cell Types

y efficient method for transfecting primary and hard-to-transfect cells with siRNAs. This was demonstrated here using five different cell types. The results show that transfection via electroporation provides an efficient nonviral method to induce RNAi in cells that are resistant to siRNA delivery using chemical transfection agents. However, conditions vary with cell type and it is important to optimize siRNA concentration and pulse number to achieve optimal silencing. Delivery of siRNA to primary cell types, a setting where genetic manipulations have traditionally proved difficult, will be a valuable research tool in various applications including gene function analysis, target validation, gene discovery, and even development of gene-specific siRNA-based therapeutics.


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Cat# Product Name Size 8990 siPORT siRNA Electroporation Buffer 12 x 1.5 ml
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