Navigation Links
Delivering siRNAs to Difficult Cell Types

Electroporation of Primary, Neuronal and Other Hard-to-transfect Cells

Dmitriy Ovcharenko, Rich Jarvis, Kevin Kelnar, David Brown, Ambion, Inc.


RNA interference (RNAi) is a powerful experimental tool for reducing the expression of specific genes. It is used routinely for gene function analysis, target validation, and gene discovery. RNAi is also exploited to create new gene-specific therapeutics. Scientists performing RNAi experiments in mammalian systems must deliver siRNAs into cells where the siRNAs guide the RNA-induced silencing complex (RISC) to target mRNA for cleavage. Lipid-based transfection reagents are typically used for siRNA delivery in immortalized cell lines. However these reagents tend to be inefficient for siRNA delivery to most primary and neuronal cell types and to cells grown in suspension. Here we demonstrate the use of electroporation to deliver siRNAs into primary cells and other hard-to-transfect cell types. Ambion's siPORT siRNA Electroporation Buffer can be used with commonly available electroporators to ensure highly efficient siRNA delivery into cells while maintaining high levels of cell viability.


An Alternative to Lipid-based Transfection
Mammalian cells can be successfully loaded with exogenous siRNA when the correct method and matrix of transfection conditions are employed. Chemical transfection (e.g. using lipid-based reagents) is used routinely to del
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Delivering siRNA Using Adenoviral Vectors
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Five Ways to Produce siRNAs
5. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
6. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
7. Optimizing Chemical Transfection and Electroporation of siRNAs
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Delivering siRNAs Difficult Cell Types

(Date:4/30/2015)... CA (PRWEB) April 30, 2015 SureClinical, ... that Julie I. Morris has joined SureClinical’s executive team ... an experienced biotech and technology executive with over 25 ... Fortune 500 companies. , Ms. Morris joins SureClinical ... biotech company. She was CFO of MBI from its ...
(Date:4/29/2015)... 2015 According to a new ... Product (Portable & Non-Portable), Component (Accelerometer, Proximity Probe, ... Metal & Mining, & Others), & Geography - ... expected to reach $1,453.76 Million by 2020, at ... data tables and 92 figures spread through 205 ...
(Date:4/29/2015)... , April 29, 2015   Doximity , ... of all US doctors as members, announced a new, ... — "Apply Using Doximity." With one click, all US ... a curriculum vitae (CV) for an open job opportunity, ... directly. Eighty seven percent of physicians are ...
(Date:4/29/2015)... Poway, CA (PRWEB) April 29, 2015 ... proud to announce that its regenerative stem cell therapy ... founded in 2002, seeking to discover a successful treatment ... ligaments. , In 2003 VetStem signed a worldwide exclusive ... veterinary application, and the first horse was treated in ...
Breaking Biology Technology:Julie I. Morris Joins SureClinical as Chief Financial Officer 2Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 2Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 3Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 4Doximity Offering Hospitals Easy, Efficient Physician Application Tool 2VetStem Biopharma -Regenerative Cell Therapy has processed over 11,000 samples 2
... BROOKFIELD, Wis.- Fiserv, Inc., an ATM and ... transfer network saw its volume increase more than 57% ... overall growth rate of 57% was tremendous and makes ... said Kevin Gregoire, Senior Vice President of Products and ...
... the great rock concert halls and rock festivals of the ... musical Meccas continues (mostly in the form of Farewell Tours ... wild 1980s and 1990s is celebrated every year in a ... lead the charge in promoting the hot biotech companies of ...
... outsourcing as an issue du jour. I stand corrected. ... year at that for America) due to slow job ... the U.S. Prominent voices along every point of the ... nomination to the Bush Administration and a host of ...
Cached Biology Technology:The Biotech Concert Halls of the Decade of 2000 2The Great Offshore Outsourcing Debate 2
(Date:4/2/2015)... In the current eight ... the strongest competitors, showing outstanding performance in recognition ... is capable of a throughput of 5.000.000 fingerprints ... be ten times faster than most competitors and ...      (Photo: http://photos.prnewswire.com/prnh/20150402/738673-a ) , ...
(Date:4/2/2015)... Fingerprint Cards (FPC) has received ... the distributor World Peace Industrial Group (WPI), part of ... the major part of the shipments will take place ... smartphone manufacturers in China . The ... communicated revenue guidance of + 1 000 MSEK for 2015.  ...
(Date:3/31/2015)... , March 31, 2015  Elephant Talk Communications Corp. ... global provider of Software Defined Network Architecture (ET Software ... total revenue of approximately $20.4 million for the year ... $19.5 million for the year ended December 31, 2013. ... the Company had completed its multi-year transition away from ...
Breaking Biology News(10 mins):DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 110 Million 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... it,s not always the good-looker who gets the girl. In ... and stealth beat out colorful and refined almost every time. ... South American species of fish ( Poecilia parae ), which ... how the interplay between male mating strategies and predator behavior ...
... researchers have found that native pollinators, like wild bees ... as honey bees and that these viruses are transmitted ... in PLoS ONE, an online open-access journal for the ... This multi-institutional study provides new insights into viral infections ...
... To survive in a tumultuous environment, sea urchins literally eat ... the spiny creatures hide from predators and protect themselves from ... where they live. The rock-boring behavior is astonishing, scientists ... grinding and scraping on stone, urchin teeth never, ever get ...
Cached Biology News:SU scientists find that in the evolutionary mating game, brawn and stealth rule 2SU scientists find that in the evolutionary mating game, brawn and stealth rule 3Movement and threat of RNA viruses widespread in pollinator community 2Ever-sharp urchin teeth may yield tools that never need honing 2
... The Amplifluor UniPrimer-fluorescein is a hairpin ... transfer pair (fluorescein and non-fluorescent quencher) ... The UniPrimer is identical to ... Universal Detection System (S7901) and is ...
...
Mouse monoclonal antibody raised against a partial recombinant EBI3. NCBI Entrez Gene ID = EBI3...
... Model AS-2055 and AS-2057 are fully automatic ... highest possible level of precision. Sampling flexibility ... positions (microplate) for,laboratory automation and combinatorial chemistry. ... micro-vials or the standard rack for 50 ...
Biology Products: