Navigation Links
Delivering siRNA Using Adenoviral Vectors

ive preparations of recombinant adenovirus, except the siRNA template oligonucleotide and the HEK 293 packaging cells (HEK 293 cells are available from several sources, including ATCC). The kit includes linearized Shuttle Vector 1.0-CMV (20 rxns), Negative Control Shuttle Vector that encodes a scrambled siRNA sequence, a Positive Control Oligonucleotide Insert that encodes an siRNA targeting GAPDH, and the Adenoviral Backbone that includes a LacZ sequence for ready monitoring of transfection efficiency. Also included are reagents for transfecting the HEK 293 cells with the Shuttle Vector and Backbone, forward and reverse sequencing primers to verify clones, and 5X Annealing Buffer for preparing the siRNA-encoding oligonucleotides for ligation.

The use of these materials is permitted for research purposes only. Any other use requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.


back to top


Ordering Information
Cat# Product Name Size 5790 pSilencer adeno 1.0-CMV System 5 virus preparations
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Delivering siRNAs to Difficult Cell Types
2. Custom and library siRNA for efficient gene silencing
3. Custom and library siRNA for efficient gene silencing
4. Cancer siRNA Oligo Set Version 1.0
5. Library siRNA
6. Custom siRNA Oligo Synthesis Service
7. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
8. Quantification of siRNA Silencing Efficiency Using the LightCycler System
9. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
10. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
11. siRNA transfection optimization with the Agilent 2100 bioanalyzer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Delivering siRNA Using Adenoviral Vectors

(Date:1/22/2015)... Jan. 22, 2015  Transwestern | RBJ today announces the firm ... space for Shire a leading biopharmaceutical company, at Two Ledgemont ... RBJ,s Robert Richards , president, and Brian Cohen ... the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... Pipette.com has added the Eppendorf ... portfolio of Eppendorf products. , The Eppendorf Centrifuge 5424/5424 ... 5424/5424 R and receive the following:, , ... or Eppendorf Reference 2 ,     3 Free ...
(Date:1/22/2015)... January 22, 2015 Diagenode, Inc., ... as the Bioruptor® and complete solutions for epigenetics ... chromatin immunoprecipitation, alleviating the need for manual processing. ... needed for ChIP of histones or transcription factors ...
(Date:1/22/2015)... N.J. , Jan. 22, 2015  Derma Sciences, ... regeneration company focused on advanced wound care, announces ... placental tissues, have been added to the Premier, ... increases the opportunity for the AMNIOEXCEL® and AMNIOMATRIX® ...
Breaking Biology Technology:Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5
... 17, 2011 Hadasit Bio-Holdings, Ltd. - (OTCBB: HADSY), ... on intellectual property developed by the Hadassah University Hospital, ... company Enlivex Therapeutics (92% owned by Hadasit Bio) has ... in its Phase I / II clinical trial using ...
... ReportsnReports   announces ... Recovery Solutions, Electric Vehicle, Telemedicine, NFC PoS Terminal, 3D ... Technoavio / Infiniti Research has recently ... Priced differently based on the market and geography researched, ...
... Institute for Biotechnology) and Ghent University have shown that ... rheumatoid arthritis, an often-crippling inflammation of the joints that ... the underlying molecular mechanism of the disease was largely ... , the researchers demonstrate that a cell-specific defect in ...
Cached Biology Technology:Clinical Developments at Enlivex Therapeutics - a Hadasit Bio-Holdings Portfolio Company 2Clinical Developments at Enlivex Therapeutics - a Hadasit Bio-Holdings Portfolio Company 3Clinical Developments at Enlivex Therapeutics - a Hadasit Bio-Holdings Portfolio Company 4ReportsnReports - Global Hospital Information Systems, Mobile Health Applications, Electric Vehicle Market Research Reports 2ReportsnReports - Global Hospital Information Systems, Mobile Health Applications, Electric Vehicle Market Research Reports 3ReportsnReports - Global Hospital Information Systems, Mobile Health Applications, Electric Vehicle Market Research Reports 4ReportsnReports - Global Hospital Information Systems, Mobile Health Applications, Electric Vehicle Market Research Reports 5Defect in A20 gene expression causes rheumatoid arthritis 2
(Date:12/22/2014)... Conn. , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile commerce market, ... Apollo Robbins for the 2015 International CES debut ... Apollo Robbins will be at the NXT-ID booth January ...
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ... "Micro Market Monitor: North America Perimeter Security Systems Market" ... The North American perimeter security market is ... to 2019. Although the U.S. market holds a larger share ...
(Date:12/17/2014)... , Dec. 15, 2014 The Defense Logistics Agency ... detect and prevent counterfeit microcircuits from entering into its ... an in-house microcircuit anti-counterfeit initiative dubbed DNA marking. The ... increasing their reliability throughout the supply chain. The new ...
Breaking Biology News(10 mins):NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3
... other medicines inside soil microbes and other natural sources is ... than 50 percent of today,s prescription drugs. This new era ... Chemical & Engineering News , the weekly newsmagazine of ... editor, points out that natural products are the source of ...
... genomes provide useful medical information beyond what current newborn ... question are being funded by the Eunice Kennedy Shriver ... and the National Human Genome Research Institute (NHGRI), both ... $5 million to four grantees have been made in ...
... controlled study of science faculty at minority-serving institutions ... increased the number of peer-reviewed research publications, the ... professional and curricular activities of those who participated ... The study, published today in the journal, ...
Cached Biology News:NIH program explores the use of genomic sequencing in newborn healthcare 2NIH program explores the use of genomic sequencing in newborn healthcare 3NIH program explores the use of genomic sequencing in newborn healthcare 4Outside mentoring support for science faculty at minority-serving institutions pays off 2Outside mentoring support for science faculty at minority-serving institutions pays off 3Outside mentoring support for science faculty at minority-serving institutions pays off 4
... gene in baculoviral expression vector, transfection ... purification of ten clones, generation of ... clone, protein expression and tag purification ... yield and timeline: 1-4 mg of ...
... Synthesis Service provides you with ... knockdown any gene. We offer ... pairs with terminal dT nucleotides), ... lengths of your choice. Each ...
EDG-1 Western blot control Source: Cell Lysate Application: Western Blotting Control...
Hybridization cassette for one slide...
Biology Products: