ive preparations of recombinant adenovirus, except the siRNA template oligonucleotide and the HEK 293 packaging cells (HEK 293 cells are available from several sources, including ATCC). The kit includes linearized Shuttle Vector 1.0-CMV (20 rxns), Negative Control Shuttle Vector that encodes a scrambled siRNA sequence, a Positive Control Oligonucleotide Insert that encodes an siRNA targeting GAPDH, and the Adenoviral Backbone that includes a LacZ sequence for ready monitoring of transfection efficiency. Also included are reagents for transfecting the HEK 293 cells with the Shuttle Vector and Backbone, forward and reverse sequencing primers to verify clones, and 5X Annealing Buffer for preparing the siRNA-encoding oligonucleotides for ligation.

The use of these materials is permitted for research purposes only. Any other use requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

back to top

Ordering Information
Cat# Product Name Size 5790 pSilencer adeno 1.0-CMV System 5 virus preparations
'"/>

Source:

Page: All 1 2 3 4
Related biology technology :

1. Delivering siRNAs to Difficult Cell Types
2. Custom and library siRNA for efficient gene silencing
3. Custom and library siRNA for efficient gene silencing
4. Cancer siRNA Oligo Set Version 1.0
5. Library siRNA
6. Custom siRNA Oligo Synthesis Service
7. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
8. Quantification of siRNA Silencing Efficiency Using the LightCycler System
9. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
10. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
11. siRNA transfection optimization with the Agilent 2100 bioanalyzer