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Delivering siRNA Using Adenoviral Vectors


Efficiently deliver an siRNA expression construct into a variety of mammalian cells and organisms

Ideal for many primary and other difficult to transfect cells

Modified CMV promoter efficiently expresses hairpin siRNAs

Positive Control Oligonucleotide Insert and Negative Control Shuttle Vector included


Efficient delivery of siRNA or an siRNA expression construct into cells represents a critical step in most RNA interference (RNAi) based gene silencing experiments. While traditional lipid and amine based reagents work well for transfecting some cultured cells, they can be inefficient for transfecting many primary cells, neural cells, etc. Viral delivery is one way to overcome this limitation. In addition, viral vectors permit the delivery of siRNA expressing constructs into a variety of animal species.

Adenoviruses are popular gene delivery vehicles because they efficiently transduce many different cell types, including hard-to-transfect terminally differentiated cells. Also, adenovirus gene delivery typically results in higher levels of RNA expression. Infection is independent of cell cycle, so adenoviruses can be used to express RNA in both dividing and non-dividing cells. Integration of the adenoviral DNA into the host genome is rare, which means that there is little chance of insertional mutagenesis. Because of this feature and the fact that most recombinant adenoviruses elicit an immune response in ani
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