Navigation Links
Deliver siRNAs Into Primary Cells

tained (Figure 3). Ambion scientists have optimized electroporation conditions for many different cell types. These electroporation conditions are available on our Electroporation Buffer webpage (see uppermost data table on right side of page).

Figure 3. Cell Viability and Reduction of GAPDH Gene Expression in Several Cell Types. Successful gene silencing and high cell viability was achieved in 11 primary cell types and 3 hard-to-transfect immortalized cell lines: Primary Cells: human mesenchymal stem cells (hMSC), normal human astrocyte cells (NHA), normal human dermal fibroblasts-neonatal (NHDF-Neo), rat astrocytes (DI TNC1), normal human umbilical vein endothelial cells (HUVEC), bovine aortic endothelial cells (BAEC), bovine aortic vascular smooth muscle cells (BAVSMC), bovine adrenal microvascular endothelial cells (BAMEC), mouse embryo fibroblast (MEF), rhesus monkey stem cells (RMSC), bovine lung microvascular endothelial cells (BLMVEC) are shown in blue. Hard-to-transfect cell lines: Jurkat (human acute T-cells), K562 (human erythroleukemia cells), PC12 (rat pheochromocytoma cells) are shown in black.

siRNA targeting GAPDH or a negative control siRNA (1.5 g) were electroporated. 48 hours after transfection, the cells were harvested and analyzed by real time RT-PCR for gene expression levels. 18S rRNA levels were used to normalize GAPDH expression. Remaining gene expression was calculated as a percentage of gene expression compared with the negative control siRNA.


Summary
Ambion's siPORT siRNA Electroporation
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
2. High-Titer Retroviral Vectors for Gene Delivery
3. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
4. A New Way to Assess siRNA Delivery Efficiency
5. Delivering siRNA Using Adenoviral Vectors
6. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
7. Delivering siRNAs to Difficult Cell Types
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
11. Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Deliver siRNAs Into Primary Cells

(Date:5/6/2015)... May 6, 2015 ... das Unternehmen einen mehrjährigen Vertrag mit Quercus ... Unternehmen exklusiv für den Vertrieb der Familie ... konventionellen und organischen Wein- und Mosthefestämme in ... sofort in Kraft. "Quercus genießt ...
(Date:5/6/2015)... San Jose, California (PRWEB) May 06, 2015 ... – Quantum Dots, a type of nanomaterial with ... the fastest-growing product in the global nanophotonics materials ... properties of quantum dots due to quantum confinement ... use in a wide range of scientific, technological, ...
(Date:5/6/2015)... Santa Clara CA (PRWEB) May 06, 2015 ... microscopy (AFM) today announced NX-Hivac, the only high vacuum ... and future needs for failure analysis semiconductor manufacturing. Park ... are interested in failure analysis solutions in highly doped ... are now required. , The high vacuum scanning ...
(Date:5/5/2015)... MD (PRWEB) May 05, 2015 RURO, ... provider released FreezerPro® version 7, the latest major content ... software., Upgrade is a significant ... UI succeeds in enhancing users experience, while also simplifying ... performance, reduces system requirements , Download available ...
Breaking Biology Technology:Renaissance Yeast Inc. ernennt Quercus Europe, S.L. zum exklusiven Fachhändler für Schwefelwasserstoff-vermeidende Wein- und Mosthefen in Spanien 2Expanding Applications & Full-Scale Commercialization to Drive the Global Quantum Dots Market, According to a New Report by Global Industry Analysts, Inc. 2Expanding Applications & Full-Scale Commercialization to Drive the Global Quantum Dots Market, According to a New Report by Global Industry Analysts, Inc. 3Park Systems Introduces Park NX-Hivac, a High Vacuum SSRM AFM System for Optimal Results in Semiconductor Manufacturing Failure Analysis 2Park Systems Introduces Park NX-Hivac, a High Vacuum SSRM AFM System for Optimal Results in Semiconductor Manufacturing Failure Analysis 3RURO Releases version 7 of FreezerPro® software 2
... , a provider of education products in Wisconsin Rapids, ... president, John Stearns. , ,Generation21 lost $4.6 million before ... Learning said it had looked for strategic alternatives over ... Stearns was its best option. , ,The company will ...
... Reserve Bank, is famous for, among other things, saying ... Recently however, he managed to speak quite plainly (by ... within the American workforce. Here's what the Fed chief ... on February 12, 2005 (See his complete statement ...
... Information technology professionals might know technology, but business ... at the Fusion 2005 CEO-CIO Symposium said as ... examined ways a chief information officer can hold sway in ... knowledge into decision making, said John Byrnes, managing director of ...
Cached Biology Technology:Is America prepared to win the global skills race? 2Is America prepared to win the global skills race? 3Is America prepared to win the global skills race? 4CIOs learn boardroom-speak 2CIOs learn boardroom-speak 3
(Date:4/2/2015)... , April 2, 2015 ... tests DERMALOG by far outperforms the strongest competitors, ... maintaining high accuracy, the system is capable of ... the speed proves up to be ten times ... than its runner-up performance.      (Photo: ...
(Date:4/1/2015)...  NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... growing mobile commerce market, announces the second shipment of ... to early access pre-order customers. Feedback ... outlets including Walmart, Target, AT&T, Dunkin, Donuts, Stop & ... at all outlets and very easy to use. Several ...
(Date:3/30/2015)... and Markets ( http://www.researchandmarkets.com/research/lc9bnl/global_gesture ) has announced ... in Automotive Sector 2014-2018" report to their offering. ... market in Automotive Sector to grow at a CAGR ... recognition is the ability of a device to identify ... recognition technology can be 2D-based or 3D-based. It uses ...
Breaking Biology News(10 mins):DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4Global Gesture Recognition Market in Automotive Sector Report 2015 - Growing Number of Partnerships 2
... Clonorchis sinensis affects more than 35 million people in ... this parasite causes clonorchiasis. Repeated or chronic infection can lead ... ducts, including the frequently fatal bile duct cancer - cholangiocarcinoma ... sinensis , published in BioMed Central,s open access journal ...
... Bangladesh, Vietnam and parts of the United States and United ... tolerance of crops to partial or complete submergence is a ... cannot survive a flood for long periods of time, leading ... at the University of California, Riverside and The University of ...
... the Beautiful: Art, Science, and Evolution" (Bloomsbury Press) will be ... have called the book a freewheeling discussion of the role ... to be found in the great diversity of life. ... animals have a natural aesthetic sense, Rothenberg probes why animals, ...
Cached Biology News:Liver parasite lacks key genes for fatty acid synthesis: Genome sequencing of Clonorchis sinensis 2How plants sense low oxygen levels to survive flooding 2How plants sense low oxygen levels to survive flooding 3New book from NJIT professor focuses on art, science and evolution 2
... U. Desselberger (1995) • This ... brings together classical and molecular techniques ... virus infections and analysis of virus-specific ... protocols are presented covering immunoassays, blotting ...
...
... Kits for Sequencing allow 5-minute cloning and subsequent ... Cloning vectors with primer sites located as close ... insertion site. This minimizes the amount of vector ... the sequence of your insert.vectors are available. The ...
Blot Paper 7 x 8.5cm semi-dry western blots (order number RB-400)...
Biology Products: