Navigation Links
Deliver siRNAs Into Primary Cells

tained (Figure 3). Ambion scientists have optimized electroporation conditions for many different cell types. These electroporation conditions are available on our Electroporation Buffer webpage (see uppermost data table on right side of page).

Figure 3. Cell Viability and Reduction of GAPDH Gene Expression in Several Cell Types. Successful gene silencing and high cell viability was achieved in 11 primary cell types and 3 hard-to-transfect immortalized cell lines: Primary Cells: human mesenchymal stem cells (hMSC), normal human astrocyte cells (NHA), normal human dermal fibroblasts-neonatal (NHDF-Neo), rat astrocytes (DI TNC1), normal human umbilical vein endothelial cells (HUVEC), bovine aortic endothelial cells (BAEC), bovine aortic vascular smooth muscle cells (BAVSMC), bovine adrenal microvascular endothelial cells (BAMEC), mouse embryo fibroblast (MEF), rhesus monkey stem cells (RMSC), bovine lung microvascular endothelial cells (BLMVEC) are shown in blue. Hard-to-transfect cell lines: Jurkat (human acute T-cells), K562 (human erythroleukemia cells), PC12 (rat pheochromocytoma cells) are shown in black.

siRNA targeting GAPDH or a negative control siRNA (1.5 g) were electroporated. 48 hours after transfection, the cells were harvested and analyzed by real time RT-PCR for gene expression levels. 18S rRNA levels were used to normalize GAPDH expression. Remaining gene expression was calculated as a percentage of gene expression compared with the negative control siRNA.


Summary
Ambion's siPORT siRNA Electroporation
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
2. High-Titer Retroviral Vectors for Gene Delivery
3. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
4. A New Way to Assess siRNA Delivery Efficiency
5. Delivering siRNA Using Adenoviral Vectors
6. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
7. Delivering siRNAs to Difficult Cell Types
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
11. Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Deliver siRNAs Into Primary Cells

(Date:5/22/2013)... Industry leaders from around the ... Oak Ridge National Laboratory’s Biosurveillance Symposium ... the Hilton Alexandria Mark Center. Held in conjunction ... conference on June 18 - 19. , Join ... Elements, Applied Research Associates, ARA, AttaGene, Battelle, Benedictine ...
(Date:5/22/2013)... May 22, 2013  Empire Genomics announced today ... patent pending novel genomic biomarker from Cornell University ... that could help in diagnosing and determining treatment ... Neuroendocrine prostate cancer (NEPC) is a ... arise de novo, but more commonly arises after ...
(Date:5/22/2013)... Toronto, Ontario (PRWEB) May 22, 2013 ... of advanced sound therapy for tinnitus treatment. AudioNotch ... Destination Hearing . With clinic locations in both ... trusted provider of audiology and hearing services. AudioNotch's ... Zabell locations. , Upon entering a Destination Hearing ...
(Date:5/22/2013)... FL (PRWEB) May 22, 2013 A recent ... of probiotics on athletes to find that those who took ... longer. The results from the study showed that in extreme ... Action, known for their adult acne treatment explains ... and how it may help their skin condition. , ...
Breaking Biology Technology:Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 2Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 3Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 4Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 5Empire Genomics Licenses Novel DNA Biomarker for Use in Diagnosing and Creating a Companion Diagnostic Test for Neuroendocrine Prostate Cancer 2AudioNotch Tinnitus Treatment Launches Partnership With Destination Hearing 2Adult Acne Treatment, Probiotic Action Shares Insight on New Findings Between Probiotics and Heat Tolerance, and What It Means for Acne Sufferers 2
... China Medical,Technologies, Inc. (the "Company") (Nasdaq: ... develops, manufactures and markets advanced,in-vitro diagnostic products, today ... Bladder Cancer FISH Probe (the "Probe") from the ... People,s Republic of China. , ...
... providing the highest quality imaging. , ... Mill Creek, ... (a division of Westover Scientific, Inc.) announced today the launch of ... , , , ,The EVOS platform offers a fully integrated digital ...
... Conn. As researchers push towards detection of single ... mass and movement, Yale researchers have demonstrated silicon-based nanocantilevers, ... photonic principles eliminating the need for electric transducers and ... April 26 advance online publication of Nature Nanotechnology ...
Cached Biology Technology:AMG Launches EVOS fl, an 'Evolutionary' Fluorescence Microscope Based on Innovative EVOS Platform 2Catching the lightwave: Nano-mechanical sensors 'wired' by photonics 2
(Date:5/22/2013)... developed a novel technique that can detect molecular variants ... is one of the most important, though time-consuming, processes ... paper in Nature , post-doctoral researcher David Patterson, ... of the Center for Free-Electron Laser Science (CFEL) in ... microwave fields to identify molecular variants apart, and to ...
(Date:5/22/2013)... discovery of potential environmental and human health effects ... each year has led scientists to recommend stronger ... of lithium-ion (Li-ion) battery materials. That,s the conclusion ... Environmental Science & Technology . , Oladele ... batteries have become mainstays for powering everything from ...
(Date:5/22/2013)... for prostate cancer could become as easy for men ... UC Irvine research published today in the Journal ... than a decade of work, UC Irvine chemists have ... for prostate cancer in urine, meaning that the disease ... at dramatically lower cost. The same technology could potentially ...
Breaking Biology News(10 mins):Detecting mirror molecules 2Detecting mirror molecules 3UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3
... When most people in the developed world think ... dim memory of a vaccination at a pediatrician,s office. ... North America and much of Europe, researchers remain interested ... because improving the measles vaccine could help eliminate the ...
... A simple technique to make a common virus-killing material ... labs of Andrew Barron and Qilin Li. Rather than ... put it into the public domain. They hope wide adoption ... The Rice professors and their team reported in Environmental ...
... Genetics Society of America (GSA) is pleased to announce ... 2011 at the Town & Country Resort & Conference ... together basic research scientists, who study genetic models in ... hereditary traits Thomas Hunt Morgan first studied nearly a ...
Cached Biology News:Measles virus, a weapon against cancer? 2Virus killer gets supercharged 2Virus killer gets supercharged 352nd Genetics Society of America Drosophila Research Conference announced 2
... new MALDI O-TOF creates a new category ... orthogonal platform that has been licensed to ... of Manitoba. , As the first single ... and collisional cooling, the prOTOF 2000 represents ...
Rat Lung Fibroblasts (RLF) (>500,000 cells)...
Rat Aortic Endothelial Cells (RAOEC) (>500,000 cells)...
...
Biology Products: