Navigation Links
Deliver siRNAs Into Primary Cells

tained (Figure 3). Ambion scientists have optimized electroporation conditions for many different cell types. These electroporation conditions are available on our Electroporation Buffer webpage (see uppermost data table on right side of page).

Figure 3. Cell Viability and Reduction of GAPDH Gene Expression in Several Cell Types. Successful gene silencing and high cell viability was achieved in 11 primary cell types and 3 hard-to-transfect immortalized cell lines: Primary Cells: human mesenchymal stem cells (hMSC), normal human astrocyte cells (NHA), normal human dermal fibroblasts-neonatal (NHDF-Neo), rat astrocytes (DI TNC1), normal human umbilical vein endothelial cells (HUVEC), bovine aortic endothelial cells (BAEC), bovine aortic vascular smooth muscle cells (BAVSMC), bovine adrenal microvascular endothelial cells (BAMEC), mouse embryo fibroblast (MEF), rhesus monkey stem cells (RMSC), bovine lung microvascular endothelial cells (BLMVEC) are shown in blue. Hard-to-transfect cell lines: Jurkat (human acute T-cells), K562 (human erythroleukemia cells), PC12 (rat pheochromocytoma cells) are shown in black.

siRNA targeting GAPDH or a negative control siRNA (1.5 g) were electroporated. 48 hours after transfection, the cells were harvested and analyzed by real time RT-PCR for gene expression levels. 18S rRNA levels were used to normalize GAPDH expression. Remaining gene expression was calculated as a percentage of gene expression compared with the negative control siRNA.


Summary
Ambion's siPORT siRNA Electroporation
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
2. High-Titer Retroviral Vectors for Gene Delivery
3. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
4. A New Way to Assess siRNA Delivery Efficiency
5. Delivering siRNA Using Adenoviral Vectors
6. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
7. Delivering siRNAs to Difficult Cell Types
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
11. Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Deliver siRNAs Into Primary Cells

(Date:8/1/2014)... Aug. 1, 2014 One of the major ... personalized medicine, which is the practice of using genomic ... a patient,s individual biology. Personalized diagnostics—laboratory analyses that inform ... in the lab,s future. This fall, AACC ,s ... latest science in this exciting field and the novel ...
(Date:8/1/2014)... BCC Research ( http://www.bccresearch.com ) reveals in its ... MARKETS , that the global market RNA interference (RNAi) ... $38.8 billion by 2018, with a five-year compound annual ... the fastest moving segment of the market, is moving ... RNAi therapeutics has shown tremendous growth and has moved ...
(Date:7/31/2014)... (PRWEB) August 01, 2014 ... China Polyurethane Resin Industry” is a professional ... market. The report introduces Polyurethane Resin basic ... industry chain structure and industry overview. This ... China’s domestic market as well as global ...
(Date:7/31/2014)... see through organs and even the entire body to ... cellular structures has been a long-time dream of biologists. ... 31st in the journal Cell has now ... making opaque organs, bodies, and human tissue biopsies transparent, ... protocols could pave the way for a better understanding ...
Breaking Biology Technology:First-Ever AACC Virtual Conference to Focus on Personalized Diagnostics 2Global Market for RNAi Drug Delivery Technologies to Reach $38.8 Billion in 2018; Aptamer Delivery Segment Surging at 58.2% CAGR 2Global Market for RNAi Drug Delivery Technologies to Reach $38.8 Billion in 2018; Aptamer Delivery Segment Surging at 58.2% CAGR 3Polyurethane Resin Industry & Thermoplastic Polyurethane Market (China, Global) Analysis Now at DeepResearchReports.com 2Polyurethane Resin Industry & Thermoplastic Polyurethane Market (China, Global) Analysis Now at DeepResearchReports.com 3Polyurethane Resin Industry & Thermoplastic Polyurethane Market (China, Global) Analysis Now at DeepResearchReports.com 4See-through organs and bodies will accelerate biomedical discoveries 2
... , SAN FRANCISCO, Aug. 11 ... stage biopharmaceutical company developing a proprietary breakthrough class of targeted ... private placement financing with gross proceeds of US $5,123,521, exceeding ... anticipated on or before September 15, 2009, with additional proceeds ...
... , , CAMBRIDGE, Mass., Aug. 11 ... of novel therapies for immune-mediated diseases, today announced changes to the protocol ... and efficacy of otelixizumab, a targeted T cell immunomodulator, in patients with ... ( D urable Response Therapy E valuation F or ...
... SEATTLE, Aug. 11 Omeros Corporation today announced ... OMS302, the Company,s ophthalmologic PharmacoSurgery(TM) product candidate. OMS302 is ... agent that causes pupil dilation (mydriasis), each with well-known ... Omeros, Phase 1/Phase 2 trial enrolled 61 patients undergoing ...
Cached Biology Technology:Jennerex Closes $5 Million First Tranche of Series C Financing 2Tolerx Announces Amendment to Phase 3 DEFEND Protocol 2Tolerx Announces Amendment to Phase 3 DEFEND Protocol 3Omeros Announces Positive Clinical Results of Phase 1/Phase 2 Trial of OMS302 for Use During Ophthalmologic Surgery 2
(Date:7/31/2014)... enormous conflagration known as the Rim Fire was in ... mature trees, when it entered the backcountry of Yosemite ... 2013. But inside the park, the battle began to ... decisions and drought can combine to fuel large, severe ... it eventually encountered lands where fire had been used ...
(Date:7/31/2014)... are familiar with Hibiscus flowers- they are an ... are commonly planted in the landscape. Some, like ... endangered species. , Only a relatively few botanists ... an equally beautiful and intriguing related group of ... of Hibiscus". , Brother of Hibiscus species are ...
(Date:7/31/2014)... White, brown and beige adipocytes, or fat cells, are ... functions and each plays its own role in metabolism. ... far the most prevalent. Its primary function is energy ... energy to generate heat but are only found in ... adipocytes, which represent a special type of brown adipocytes, ...
Breaking Biology News(10 mins):The Rim Fire 1 year later: A natural experiment in fire ecology and management 2The Rim Fire 1 year later: A natural experiment in fire ecology and management 3The Rim Fire 1 year later: A natural experiment in fire ecology and management 4The Rim Fire 1 year later: A natural experiment in fire ecology and management 5Brother of Hibiscus is found alive and well on Maui 2Hope for the overweight 2
... Washington, DC Engineered nanoscale materials (ENMs), which ... use in food packaging, raise new safety evaluation ... report released today by the Project on Emerging ... The food-packaging industry, food companies and ...
... developed an effective technology for reducing contamination of dangerous ... Salmonella and E. coli O157:H7 on foods ranging from ... It is made from inexpensive and readily available ingredients ... and Drug Administration. The new technology, which ...
... Almost every June for 30 years, Terry McEneaney drove around ... three routes. Park ornithologist at the time, he would ... Then he,d drive another half mile, repeat the process and ... for the North American Breeding Bird Survey. Trying to finish ...
Cached Biology News:Use of nanomaterials in food packaging poses regulatory challenges 2New UGA invention effectively kills foodborne pathogens in minutes 2Bird watchers, space technology come together in Montana State University study 2Bird watchers, space technology come together in Montana State University study 3
Recombinant Rhesus Macaque IFN-alpha...
chk 1 kinase...
...
KNP-1 (HES1) Purified Anti-Mouse, Anti-Rat, Anti-Human, Anti-Dog, Anti-Chicken clone 35, Isotype Mouse IgG 1 , 150 µg Consult technical datasheet for details....
Biology Products: