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Deliver siRNAs Into Primary Cells

talog/CatNum.php?16704">Silencer Pre-designed siRNAs. These siRNAs are designed using an extensively tested algorithm developed by Cenix BioScience, and have been shown to be highly effective at silencing target genes. As seen in Figure 2, 48 hours after transfection, mRNA expression levels of the targeted genes were reduced by 70% or more for all siRNAs tested in both cell types.

Figure 2. siRNAs Electroporated into hMSC and NHDF-neo Cells. Six siRNAs (1.5 g) targeting CDK2, JAK1, p53, GAPDH, uPAR, PKR, as well as a negative control siRNA, were electroporated into hMSC cells (A). Seven siRNAs (1.5 g) targeting Cyclin D1, NFκBp65, STAT1, PKCα, JAK1, RAF1, MMP-2, and a negative control siRNA were individually electroporated into NHDF-neo cells (B). All electroporations were conducted using the siPORT siRNA Electroporation Kit. 48 hours after electroporation, the cells were harvested and analyzed by real-time RT-PCR for corresponding gene expression levels. 18S rRNA levels were used to normalize the expression data. Percent remaining gene expression was calculated as a percentage of target mRNA compared with target mRNA from cells electroporated with the negative control siRNA.


Effective Gene Silencing in Primary and Difficult-to-Transfect Cells

In Figure 3, several different cell types were electroporated with the GAPDH control siRNA using individually optimized electroporation conditions. 48 hours after electroporation, GAPDH mRNA levels were reduced by 70% or more in each cell type. Cell viability of 70% or greater was main
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