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Deliver siRNAs Into Primary Cells

siPORT siRNA Electroporation Kit
Deliver siRNA into primary cells, cells grown in suspension, and other difficult-to-transfect cells

Maintain high cell viability under a wide range of conditions

Optimize electroporation of your cells quickly with included positive and negative controls


Primary cells serve as an important model system for gene function analysis, target validation, and drug discovery since they are more similar to their in vivo counterparts than are immortalized cells. However, delivery of siRNA into these cells has proven to be a challenge. Efficient transfer of siRNA into primary cells by chemical transfection (e.g. using lipid-based reagents) works with only a few cell types [1]. Ambion's new siPORT siRNA Electroporation Buffer overcomes this problem. It can be used with commonly available electroporators to ensure highly efficient siRNA delivery into many different cell types, including primary cells, while maintaining high levels of cell viability [2].

Now, Ambion introduces the siPORT siRNA Electroporation Kit, which provides a Cy3-labeled siRNA Control, and positive (GAPDH) and negative (non-targeting) siRNA controls in addition to the siPORT siRNA Electroporation Buffer. The kit makes it easy to optimize
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