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DNA-free A NEW Method to Remove DNA

ase I activity. DNA-free also includes a novel DNase Removal Reagent to quickly eliminate the DNase after treatment. This unique reagent effectively removes all traces of DNase and divalent cations from the reaction mixture after DNA digestion is complete. The DNase/cation removal step is fast taking only three minutes to complete. After DNase digestion, The Removal Reagent is added, the tube flicked to mix, and the solution incubated for 2 minutes at room temperature. DNase and ions are bound by the DNase Removal Reagent which is spun out with a quick centrifugation leaving the RNA in the supernatant ready for RT-PCR. This simple method avoids messy organic extraction or heat inactivation of DNase I that may put your RNA at risk. DNA-free DNase Treatment & Removal Reagents are also available as components of the RNAqueous-4PCR Kit (see sidebar, "RNA Isolation for RT-PCR").


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Cat# Product Name Size 1906 DNA-free 50 rxns 1914 RNAqueous-4PCR Kit 30 rxns


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