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DNA-free A NEW Method to Remove DNA

cells in a guanidinium-based lysis solution, binding the RNA to a glass fiber filter, washing the filter to remove contaminants, and recovering the RNA in a small volume of elution solution. Finally, the RNA is treated with the DNase treatment and removal reagents included in the kit yielding RT-PCR ready RNA.

RT-PCR Experiments Using Total RNA isolated with the RNAqueous-4PCR Kit. RNA was used as template in reverse transcription (RT) reactions or in mock RT reactions that did not contain reverse transcriptase. Ten percent of the resulting cDNA was amplified by PCR using S15 primers. No PCR product is seen from the minus-RT reactions, demonstrating the lack of DNA contamination in RNA isolated using Ambion's RNAqueous-4PCR Kit. The lanes to the right of the markers show the S15 RT-PCR product from the indicated samples.

DNA-free Removes the DNA, and Then Removes the DNase

DNA-free DNase Treatment & Removal Reagents are one of Ambion's latest tools designed to simplify RNA preparation for RT-PCR. With DNA-free, genomic DNA contamination can be removed from any RNA preparation without incurring RNA loss or risk of degradation. The DNA-free DNase Treatment & Removal Reagents provide RNase-free DNase I and optimized DNase Reaction Buffer for the complete digestion of contaminating DNA in RNA samples. Ambion's DNase I is prepared with ultra pure reagents and is not released for sale until it is shown to be both highly effective at eliminating DNA, and devoid of RNase activity. The 10X DNase Reaction Buffer is designed for optimal DN
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Cells are grown in triple flasks, roller bottles, or NUNC Factory. Viability and growth curves are generated from a parallel representative culture....
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