Navigation Links
DNA-free A NEW Method to Remove DNA

inactivation of this enzyme is problematic; DNase removal methods can be inconvenient, ineffective and even detrimental to RNA integrity.

Commonly used methods for removal or inactivation of DNase after digestion include: heat inactivation, proteinase K treatment followed by phenol:chloroform extraction, chelation of essential ions with EDTA, and purification using a glass-filter binding method such as RNAqueous (see the sidebar at right, "RNA Isolation for RT-PCR). Each of these inactivation or removal methods has its drawbacks.

Heat inactivation: Probably the most common method of DNase inactivation is heat treatment, typically for 5 minutes at 75C. Although this method appears straightforward, the divalent cations in the DNase digestion buffer can cause (chemically-induced) strand scission of RNA when heated. Studies at Ambion have shown that much of an RNA sample is destroyed when heated to 80C for 5 minutes in the presence of 2.5 mM MgCl2 and 0.1 mM CaCl2 (salts typically found in DNase I digestion buffer).

Proteinase K treatment and organic extraction: Proteinase K treatment followed by phenol:chloroform extraction is probably the most rigorous method for DNase inactivation and removal, but it is time-consuming, and organic extractions often cause some sample loss. Sample loss can be minimized by back extraction of the phenol:chloroform phase, but this adds another step to an already time-consuming procedure. Additionally, many people prefer to avoid working with hazardous
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. The Worlds Best DNase Improved TURBO DNA-free
2. A New PCR-based Mycoplasma Detection Method
3. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
4. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
5. Comparison of Different Methods of mRNA Quantitation
6. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
9. Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DNA free NEW Method Remove DNA

(Date:8/21/2014)... 21, 2014 OTC Markets Group ... a biotechnology company, on its approval to list ... on OTCQX®, the best marketplace for established U.S. ... Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO ... execution of its growth strategy and achievement of ...
(Date:8/21/2014)... (PRWEB) August 21, 2014 Pursuit ... selling optimization technology and services for Life Sciences ... “Grading Pharma’s Use of New Commercial Sales Models” ... & Analytics. , The article examines some innovative ... that are being tested in the pharmaceutical market. ...
(Date:8/21/2014)... SoundConnect , a unified communication ... of the nation’s Fastest Growing Private Companies by ... consecutive year. Inc. magazine today ranked SoundConnect NO. ... exclusive ranking of the nation's fastest-growing private companies. ... the most important segment of the economy—America’s independent ...
(Date:8/20/2014)... patented university inventions licensed to biotechnology firms has ... To open these roadblocks, the researchers suggest that ... discovery stage could lead to faster commercialization down ... from discoveries made in university laboratories and licensed ... clinical trials, which have a high failure rate. ...
Breaking Biology Technology:OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2Pursuit's Peter Robinson Publishes "Grading Pharma's Use of New Commercial Sales Models" 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3Early bottlenecks in developing biopharmaceutical products delay commercialization 2Early bottlenecks in developing biopharmaceutical products delay commercialization 3
... it plans to buy its former parent AT&T , ... opportunities. , ,Drew Petersen, director of legislative affairs for ... is a sign that SBC plans to become a global ... Petersen said tries to present itself as a Midwestern company, ...
... Depressed? , ,Considering we just escaped January a ... the cold, dark and dank weather thats no ... 24 (just one week ago) was the most depressing ... bear an extra psychological burden because of the challenges ...
... biotech company moving to Madison from Chicago is being ... state. , ,Governor Jim Doyle announced earlier this week ... from the Department of Commerce Technology Venture Fund ... programs to help the company purchase new equipment and ...
Cached Biology Technology:SBC merger with AT&T could open opportunities in Wisconsin 2Post-January, tech entrepreneurs have reasons to be depressed 2Post-January, tech entrepreneurs have reasons to be depressed 3Post-January, tech entrepreneurs have reasons to be depressed 4
(Date:8/20/2014)... of Tbingen, Arizona State University, the Wellcome Trust Sanger ... (Swiss TPH) isolated Mycobacterium pinnipedii from skeletons ... old. The pathogen is a relative of the TB ... in humans today. These researchers assume that seals carried ... link to sea lions was unexpected" comments Sebastien Gagneux, ...
(Date:8/20/2014)... Using population-based screening outcomes of approximately 3 million ... four researchers at the University of Massachusetts Medical School, ... (SCID) can be successfully implemented across public health newborn ... in the Aug. 20 issue of the Journal ... showed the rate of SCID in newborns is higher ...
(Date:8/20/2014)... Institutes of Health has awarded the Oklahoma Medical Research ... research on anthrax and the bacteria,s effects on humans. ... Ph.D., and his colleagues have studied the human immune ... Centers for Human Immunology. The original funding came soon ... the heels of the terrorist attacks of Sept. 11, ...
Breaking Biology News(10 mins):Newborn screening expansion offers early diagnosis and treatment to infants with SCID 2Newborn screening expansion offers early diagnosis and treatment to infants with SCID 3$14.5 million grant awarded to continue anthrax studies 2
... Zeneca have found a new way to use solid-state NMR ... spot unwanted polymorphs in pharmaceuticals. Pharmaceuticals companies are constantly ... can actually exist in more than one form or crystal ... different ways. Now researchers at the University of Warwick and ...
... AMES, Iowa -- Forests in the nation,s Upper Midwest ... settlers. And more changes may be coming. That,s ... professor in Iowa State University,s department of Natural Resource ... "There,s been a shift in the entire ecosystem," said ...
... TRADED BROKERAGE TO UTILIZE TITANIUM,S ... SECURITY SOLUTIONS, ... Board: TTNUF), a leading biometric and security solutions,provider announced today that ... 600837). Titanium will install its,Biometrics Access Control System in Haitong,s newly ...
Cached Biology News:NMR researchers unlock hydrogen's secrets to spot polymorphism in pharmaceuticals 2Upper Midwest forests are losing diversity, complexity, ISU study finds 2Titanium Group Announces Contract With Haitong Securities 2Titanium Group Announces Contract With Haitong Securities 3Titanium Group Announces Contract With Haitong Securities 4
... The kit contains 5 ml CELLection Dynabeads ... against the human epithelial antigen EpCAM and ... 5 log enrichment of epithelial tumour cells ... Tumour cells clumps can be enriched from ...
Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera)...
Recombinant Human Erythropoietin (Tissue Culture Grade)...
Acrylamide PAGE 40% Solution, 1 l. Category: Electrophoresis Systems & Standards & Reagents, Electrophoresis Standards and Reagents, Gel Casting Chemicals....
Biology Products: