Navigation Links
DNA-free A NEW Method to Remove DNA


Now it's easy to make your RNA free of genomic DNA contamination and ready for RT-PCR. DNA-free DNase Treatment & Removal Reagents contain RNase-free DNase, and an optimized DNase digestion buffer, to ensure safe, complete removal of contaminating DNA from any RNA sample. Also included is a unique DNase Removal Reagent which, after digestion, eliminates DNase in minutes no more messy phenol extractions or heat inactivation procedures which can cause RNA loss or degradation.

DNA, contaminating RNA preparations, can serve as a template in PCR to produce a false positive signal from RT-PCR. Although false positives are easily identified by looking at the outcome of a "minus-RT" control, eliminating the DNA is not trivial. Here we discuss the problem of genomic DNA contamination in RT-PCR, the best methods to detect and remove it, and an innovative way to remove DNase I after DNase I treatment.


All Isolation Methods Result in Contaminating DNA

There is no RNA isolation method that consistently produces RNA free from genomic DNA without the use of DNase. To illustrate this, RT-PCR was performed on mouse liver RNA isolated by several different methods:

Figure 1. DNA Contamination in RNA Isolated by 5 Different Methods. Total RNA was isolated from mouse liver by the methods indicated. RNA (0.5 g) underwent RT-PCR, or simply PCR (without reverse transcription), as indicated and aliquots of each reaction were electrophoresed on a 2% agarose gel and stained with ethidium bromide.

Lane RN
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. The Worlds Best DNase Improved TURBO DNA-free
2. A New PCR-based Mycoplasma Detection Method
3. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
4. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
5. Comparison of Different Methods of mRNA Quantitation
6. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
9. Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DNA free NEW Method Remove DNA

(Date:4/17/2015)... April 17, 2015  Centrillion Technologies, a ... grant from the National Human Genome Research ... Health (NIH). The grant will help fund ... development of breakthrough genomic technologies, including phased ... substrates. Centrillion has been developing this transformative ...
(Date:4/17/2015)...  Guggenheim Securities, the investment banking and capital markets ... William Tanner as a Managing Director and ... start work at Guggenheim in May 2015 in the ... "Bill brings a wealth of biotech experience, in ... highly acclaimed healthcare team," said Matthew Johnson , ...
(Date:4/17/2015)... Madison, WI (PRWEB) April 17, 2015 ... provide sales and marketing support for the powerful ... at the Chemical Genomics Center at the Lankenau ... LCGC’s mission is to measurably improve successful ... to HTS, by offering rare, drug-like libraries available ...
(Date:4/16/2015)... 2015 Cytokinetics, Incorporated (Nasdaq: CYTK) announced ... results on Thursday, April 30, 2015 at 4:00 PM ... host a conference call at 4:30 PM Eastern Time ... outlook for the future. , The conference call will ... homepage and in the Investor Relations section of Cytokinetics’ ...
Breaking Biology Technology:Guggenheim Securities Hires Biotech Analyst William Tanner in Expansion of Healthcare Coverage 2BellBrook Labs and Lankenau Institute Partner to Accelerate Drug Discovery using Orthogonal Pooled Screening 2Cytokinetics to Announce First Quarter Results on April 30, 2015 2Cytokinetics to Announce First Quarter Results on April 30, 2015 3
... Yongye Biotechnology,International, Inc. (OTC Bulletin Board: YGYB) ("Yongye" or ... for plants and,animals in the People,s Republic of China, ... 30, 2008., Second Quarter 2008 Highlights ... from the second quarter of ...
... 17 /Xinhua-PRNewswire/ -- WuXi AppTec, Inc., the,operating company ... the U.S., has announced,the appointments of Garry Takle, ... Ph.D. as Vice President of Testing Services for ... Dr. Takle has been with the company ...
... 15 ETEX Corporation, an advanced,biomaterials company, ... innovative,orthobiologic, EquivaBone(TM). EquivaBone(TM) is a bone graft ... bone during the healing process., (Logo: ... President and CEO of ETEX Corporation, states, ...
Cached Biology Technology:Yongye Biotechnology International Announces Second Quarter Results 2Yongye Biotechnology International Announces Second Quarter Results 3Yongye Biotechnology International Announces Second Quarter Results 4Yongye Biotechnology International Announces Second Quarter Results 5Yongye Biotechnology International Announces Second Quarter Results 6Yongye Biotechnology International Announces Second Quarter Results 7Yongye Biotechnology International Announces Second Quarter Results 8Yongye Biotechnology International Announces Second Quarter Results 9Yongye Biotechnology International Announces Second Quarter Results 10Yongye Biotechnology International Announces Second Quarter Results 11WuXi AppTec Appoints Garry Takle, Ph.D. as VP of Operations and Joseph Hughes, Ph.D. as VP of Testing Services for Philadelphia Facility 2ETEX Launches Innovative Osteoinductive Orthobiologic, EquivaBone(TM) 2
(Date:3/20/2015)... Mar. 19, 2015 Research and Markets ( ... Biometrics - Global Strategic Business Report" report to their ... Iris Biometrics in US$ Thousands. The report provides separate comprehensive ... Japan , Europe , ... and Latin America . Annual ...
(Date:3/18/2015)... SPRINGS, Florida , March 18, 2015 ... well as businesses, new revolutionary smart wallets and apps continue ... pace.  Companies currently entrenched in the mobile payment industry in ... Alibaba Group Holding Ltd. (NYSE: BABA ), Apple ... GOOG ) and Facebook Inc. (NASDAQ: FB ...
(Date:3/17/2015)... SAN DIEGO , March 17, 2015 /PRNewswire/ ... and emotion analytics software, today announced general availability ... on-demand web service for the analysis of facial ... they experience advertising, media content, products and services. ... customer attention, engagement and sentiment - as derived ...
Breaking Biology News(10 mins):Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 2Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 3Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 4Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 6New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 2New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 4
... S.C. Celebrating the end to a successful peach season, ... a new peach cultivar CaroTiger. The fourth in the ... will be available to growers in January 2013. "Up ... but it earned a name during our long-term germplasm ...
... of Marine Geology and Geophysics at the University of Miami ... off the fall semester with the inauguration of sophisticated equipment ... manner. Stable isotopes are used by biogeochemists to help ... using small organisms found in sediment cores. ...
... the Deepwater Horizon drilling rig exploded on April ... professor in earth and planetary sciences at the University of ... and headed down to the Louisiana coast. Engel ... resources to conduct research they knew would be needed once ...
Cached Biology News:Clemson University peach specialist unveils CaroTiger, something to roar about 2'Clumps' of opportunities for cutting edge science 2UT researcher investigates Deepwater Horizon oil spill impact on coast 2
Tris-Hepes kit of BG-163 & BG-165 (order number BG-166)...
... Immunogen: Synthetic peptide derived from the ... protein. Specificity: Reacts with the ... Reactivity: Human Mouse (positive controls: WNT1-transfected ... lysates and mouse Mouse-1 cell lysates). ...
... new StrepMAB-Immo coated microplates can be used ... SerAla-Strep-tag II fusion proteins for ELISA or ... amounts of such proteins are bound with ... on the left) and will not elute ...
ABI 432 Resins • Gln(Trt) Peptide Synthesis Column...
Biology Products: