Navigation Links
DNA Yield with Oragene

H.C. Birnboim
DNA Genotek, Ottawa, Ontario, Canada

Oragene gives higher DNA yields than other oral collection methods. The median yield of DNA from Oragene samples is 110 g. In comparison, buccal swabs may yield as little as 1.9 g of DNA.


Introduction
The number of studies collecting genomic DNA from a large number of individuals is increasing rapidly. Non-invasive methods and techniques that permit selfcollection are preferred because they increase compliance rates and reduce costs. For this reason, many large-scale studies now use buccal cells from saliva as the source of DNA. The two most common methods are scraping the inside of the cheek with a brush and swishing the mouth with a mouthwash. Oragene is a DNA self-collection kit that is non-invasive and intuitive to use. The donor rinses his or her mouth with water to clear food particles and then spits 2 mL of saliva into the Oragene vial. Once the vial is closed, DNA is released and stabilized at room temperature.

The amount of DNA recovered from the oral cavity can vary widely depending on the collection method (Figure 1). This technical bulletin reports the amount of DNA obtained from saliva using the Oragene kit.


Materials and Methods
Saliva samples were obtained from 208 donors. Collection and purification of DNA was carried out according to protocols supplied with the Oragene kit. DNA yield was determined by the highly specific Fluorescence/DNase method (ref. 5). The F/D method quantitates DNA using SYBR Green I dye (Molecular Probes, Inc.) and DNase treatment.


Results
The DNA yield of Oragene/saliva samples from 208 donors is shown in Figure 2. The median amount of DNA was 110 g. The 25th percentile was 62 g and the 75th percentile was 158 g.


Conclusions
Oragene is a non-invasive DNA self-collection kit that can be used by untrained study subjects, including children and the elderly. The median DNA yield from Oragene/saliva samples is 110 g. This is significantly higher than other oral collection methods.


References
1. Cozier, Y., Palmer, J., and Rosenberg, L. (2003) Comparison of Methods for Collection of DNA Samples by Mail in the Black Womens Health Study. AEP. 14, 117-122.

2. Harty, L., Garcia-Closas, M., Rothman, N., Reid, Y., Tucker, M., and Hartge, P. (2000) Collection of buccal cell DNA using treated cards. Cancer Epidemiology, Biomarkers & Prevention. 9, 501506.

3. Montserrat, G., Egan, K., Abruzzo, J., Newcomb, P., Titus-Ernstoff, L., Franklin, T., et al. (2001) Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiology, Biomarkers & Prevention. 10, 687-696.

4. Le Marchand, L., Lum-Jones, A., Saltzman, B., Visaya, V., Nomura, A., and Kolonel, L. (2001) Feasibility of collecting buccal cell DNA by mail in a cohort study. 10, 701-703.

5. DNA Genotek Technical Bulletin PR003 Sept 30, DNA Quantifi cation Using the Fluorescence/DNase (F/D) Assay. 2004.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DNA Yield with Oragene

(Date:9/17/2014)... September 17, 2014 A biodegradable ... microorganisms such as bacteria, fungi, and algae into ... methane, biomass, inorganic compounds, carbon dioxide, and humus ... customer base and improved costs, biodegradable polymers are ... viable alternative to conventional polymers. Primary factors that ...
(Date:9/17/2014)... Terracon Corporation has announced the introduction of ... This addition to the company’s industry-leading line of ... and available in 125 or 250 gallon capacities. ... weeks or less. , The new pre-engineered TerraPure tank ... existing TerraPure tank line – plus quick turnaround. ...
(Date:9/17/2014)... , Sept. 17, 2014 /PRNewswire/ - SQI Diagnostics Inc. ... develops and commercializes proprietary technologies and products for advanced ... been approved for trading in the United ... is expected to commence on September 17, 2014 on ... continue to trade on the TSX Venture Exchange under ...
(Date:9/17/2014)... Beckman Coulter , a global leader in ... its agreement with Personify for an additional ... will continue to streamline the Beckman Coulter talent acquisition ... time-to-fill, time-to-source and a deep reduction to cost-per-hire. , ... based on the success of our partnership over the ...
Breaking Biology Technology:Growing Demand for Eco-Friendly Products Drives the Global Biodegradable Polymers Market, According to New Report by Global Industry Analysts, Inc. 2Growing Demand for Eco-Friendly Products Drives the Global Biodegradable Polymers Market, According to New Report by Global Industry Analysts, Inc. 3Terracon Introduces Pre-Engineered TerraPure® Tank for Ultrapure Water Applications 2SQI Diagnostics to begin Trading on the OTCQX Marketplace in the United States 2SQI Diagnostics to begin Trading on the OTCQX Marketplace in the United States 3Beckman Coulter Signs 3-year RPO Contract with Personify 2Beckman Coulter Signs 3-year RPO Contract with Personify 3
... new global standard to eradicate (100% kill) ... care facilities, including MRSA and ... ... successful,testing of its Ygiene(TM) Hospital Grade antimicrobial against,antibiotic-resistant microorganisms and spore ...
... a pioneer in,the development of cost-effective, easy-to-use gene-expression ... company,s Ziplex,System as a recipient of the magazine,s ... by The Chicago Tribune, the 45-year-old R&D 100,Award, ... known to industry,government, and academia as proof that ...
... and OSLO, Norway, Aug. 19 Omeros ... AS, a,Norwegian human antibody therapeutics company, today ... for the discovery and development of fully,human ... mannan-binding,lectin-associated serine protease-2, mediates activation of the ...
Cached Biology Technology:BioNeutral's Ygiene(TM) New Hospital Grade Disinfectant Will Save Lives and Billions of Dollars Annually at Health Care Facilities Around the World 2BioNeutral's Ygiene(TM) New Hospital Grade Disinfectant Will Save Lives and Billions of Dollars Annually at Health Care Facilities Around the World 3BioNeutral's Ygiene(TM) New Hospital Grade Disinfectant Will Save Lives and Billions of Dollars Annually at Health Care Facilities Around the World 4Xceed Ziplex(R) System for Automated Gene-Expression Analysis Wins 2008 R&D 100 Award 2Affitech and Omeros Enter Into Antibody Discovery and Development Agreement 2Affitech and Omeros Enter Into Antibody Discovery and Development Agreement 3
(Date:9/17/2014)... As the United Kingdom forms a new crime unit ... an uproar last year over horse meat being passed ... a technique for detecting meat adulteration. They describe their ... in ACS, Journal of Agricultural and Food Chemistry ... fraud is a major global economic problem. But they ...
(Date:9/16/2014)... at the Columbia Center for Children,s Environmental Health at ... to demonstrate an association between childhood asthma and prenatal ... of household products. Results appear online in the journal ... to mothers exposed during pregnancy to higher levels of ... had a 72 percent and 78 percent increase in ...
(Date:9/16/2014)... September, 2014)More than $100 trillion in cumulative public ... carbon dioxide (CO2)a 40 percent reduction of urban ... the world expands public transportation, walking and cycling ... by the University of California, Davis, and the ... , Further, an estimated 1.4 million early deaths ...
Breaking Biology News(10 mins):Phthalates heighten risk for childhood asthma 2Phthalates heighten risk for childhood asthma 3Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 2Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 3Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 4Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 5
... (HCV) infects up to 500,000 people in the UK ... the single biggest cause of people requiring a liver ... groups across Europe and the USA, scientists from The ... be a significant step towards a vaccine. Hepatitis ...
... may be to blame for the gradual extinction of ... according to Ulrich Kutschera and colleagues from the University ... in Austria. Their findings show that human-induced temperature ... of Austria may have led to the near extinction ...
... dog-walking in bushland significantly reduces bird diversity and ... practice in sensitive bushland and conservation areas. ... impacts of dog-walking have remained subjective and unresolved ... the first clear evidence that birds perceive dogs ...
Cached Biology News:Possible hepatitis C vaccine 2Role reversal as humans suck life out of leeches 2A dog in the hand scares birds in the bush 2
... C1 Plus is a modular confocal system ... with an ultra-compact and lightweight design. The ... Plusboost optical performance to 400nm andits bi-directional ... support for X and Y scan axes ...
... integrated digital microscope and camera system that ... optical adjustment or software installation. Built on ... combines simplicity and advanced technology for users ... viewing of a macro image and a ...
... The TUNEL-based assay kit provides complete ... cells for convenient detection of DNA ... sections. The incorporation of BrdU in ... biotinylated or digoxigenylated methods. Results can ...
... The Fujifilm Life Science LAS-3000 ... technology with a simplified user interface, ... a wide range of modularities. The ... applications, and the perfect standard system ...
Biology Products: