Navigation Links
DNA Stability with Oragene

H.C. Birnboim
DNA Genotek, Ottawa, Ontario, Canada

Storage of specimens by refrigeration or freezing can add significant costs to a project. Oragene eliminates these costs by allowing saliva specimens to be stored for years at room temperature without DNA degradation.


Introduction
Large population-based studies, involving hundreds to thousands of subjects, are frequently used to investigate the genetic determinants of complex diseases. Saliva is a convenient source of genomic DNA because it can be collected in a painless and non-invasive manner. For logistic reasons, samples often need to be stored prior to the extraction of DNA. Common storage methods, such as refrigeration and freezing, can add significant costs and inconvenience to a genetic study. In addition, these methods may be of limited effectiveness. For example, Ng et al. (2004) observed as much as a 30% decrease in PCR product yield from saliva samples that had been stored at -70C for 1 month.

An ideal protocol would allow saliva samples to be stored at room temperature for long periods of time with no DNA degradation. Oragene is a kit that collects and preserves DNA from saliva. This technical bulletin reports the ability of Oragene to stabilize and preserve DNA in saliva at temperatures up to 50C.


Materials and Methods
Oragene/saliva samples were collected and stored at room temperature (24C), 37C, and 50C for up to 187 days. At various time intervals, aliquots were removed and processed by the standard Oragene protocol. 200 ng of DNA from each sample was analyzed by agarose gel electrophoresis with ethidium bromide staini
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Chemical Stability of Disposables
2. Chemical Stability of the Brinkmann Bottletop Dispenser and the Brinkmann Bottletop Buret
3. Oragene and Whole Genome Amplification with GenomiPhi
4. DNA Yield with Oragene
5. Oragene and DNA sequencing with ABI PRISM
6. Oragene is compatible with TaqMan SNP genotyping
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DNA Stability with Oragene

(Date:6/30/2015)... Japan , July 1, 2015 R-Japan Co.,Ltd. ... on the Safety of Regenerative Medicine from the Ministry of ... on June 29, 2015. The fact that ... processing facility through the auditing from Pharmaceuticals and Medical Devices ... the application, allows it to provide stem cell manufacturing service ...
(Date:6/30/2015)... , June 30, 2015  NASA astronaut Kjell Lindgren ... month on his first mission to the International Space ... 8 to 9 a.m. EDT Tuesday, July 7. ... Star City, Russia . The interviews will ... Television highlighting his mission training. To schedule an ...
(Date:6/30/2015)... ... June 30, 2015 , ... With its ... sacrifice selectivity to achieve faster flow rates in comparison to nylon or polyethersulfone ... open space to allow easy liquid flow while trapping particulates as small as ...
(Date:6/30/2015)... June 30, 2015  The University of California, ... (CCE/Bio) and UC San Diego Extension announced their ... held Aug.  12 – 14, 2015, at UC ... workshop provides an intense exposure to best practices ... is well-suited to biologists, process engineers and business ...
Breaking Biology Technology:R-Japan Obtained Autologous Stem Cell Manufacturing License from the Ministry of Health, Labour and Welfare 2NASA Astronaut Preps for First Space Station Mission, Available for Media Interviews 2New Nanofiber Membrane Provides Fast, Efficient Filtration 2UC San Diego Extension to Offer Third Annual Microbial Fermentation Workshop 2
... on information technology may soon be subject to an ... of millions of dollars each, have failed to deliver ... Suzanne Jeskewitz, co-chairs of the Joint Audit Committee, said ... renovated recently are inefficient or non-functional, and appear to ...
... Madison, Wis. Fifty-two University of Wisconsin undergrads ... in prizes this Thursday and Friday for creative inventions ... ,The Schoofs Prize for Creativity and Tong Prototype Prize ... of any curriculum. At this year's Innovation Days, undergrads ...
... Crosse-based data quality firm Firstlogic for about $69 ... the second quarter. , ,Firstlogic is a privately held firm ... make sure their data is as correct as they need ... as call centers, web sites, customer relationship management systems, and ...
Cached Biology Technology:
(Date:6/17/2015)... Deutschland und GERMANTOWN, Maryland , June ... ®   Produktlinie mit DNA-Tests verbessert den ... (NASDAQ: QGEN ; Frankfurt Prime Standard: QIA) hat ... STR-Testkits der Produktlinie Investigator ® ... Markt eingeführt. Die neuen Kits zur Erstellung genetischer Fingerabdrücke ...
(Date:6/17/2015)... , June 17, 2015  Crossmatch™, a ... announced that its U.are.U ® 4500 fingerprint ... Eateries, Inc., a San ... chain, to increase security, improve accountability and reduce ... enable instant, non-repudiable identity confirmation for employee sign ...
(Date:6/16/2015)... GOTHENBURG, Sweden , June 16, 2015 ... including all touch fingerprint sensors in the company,s portfolio from ... . Deliveries are planned to mainly take place during the ... manufacturers in Asia . Jörgen ... another evidence of    the   growing interest from smartphone ...
Breaking Biology News(10 mins):QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3FPC Receives Order for Touch Fingerprint Sensors of 375 MSEK 2
... Food and Drug Administration (FDA) is warning consumers not to use ... the Internet by Globus Media, Montreal, Canada. The use of these ... adverse health consequences. The illegal kits are labeled as: Rapid HIV ... Kit , * One Step Cassette Style Cocaine ...
... be a godsend for many of the two million ... downside. People on the drug may develop hypothyroidism, tremors, ... weight. , However, better treatments for bipolar disorder depend ... which lithium damps the highs and lows of the ...
... partially this week , this initiative is so important ... In a publication today in the prestigious scientific journal, ... 'kernel' of the world's first 'explicit open source' biotechnology ... allow the public-sector, small to medium enterprises and even ...
Cached Biology News:FDA Warns Consumers Not to Use Home-Use Diagnostic Kits Marketed by Globus Media 2Beyond lithium for bipolar disorder 2The BIOS Initiative - open source biotechnology is born 2