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DNA Removal for Bioprocessing Applications

on. Quantitation of the total protein concentration for each DNase reveals that 1 unit of the competitors wild type DNase I contains 9.5 times more protein than 1 unit of TURBO DNase (data not shown). Thus, TURBO DNase demonstrated 663-fold better specific activity for DNA removal in this assay than the wild type DNase I tested (i.e. 9.5 x (201833/ 2893)=663 fold).


Cell Viability
To quantify the effect of TURBO DNase treatment on the viability of the cultured cells, representative populations were stained with Viacount dyes that distinguish between living and dead cells. The results were analyzed by microcytometry using a Guava Technologies PCA-96 instrument and Cytosoft software. Treatment of the cells with 5 units or even as much as 50 units of TURBO DNase plus enhancer did not affect cell viability as compared to non-treated cells (Figure 2). In addition, scrutiny of the cell populations with the Cytosoft analysis software showed no evidence of mid-phase apoptotic cells in either treated or untreated cells.

Figure 2. Turbo DNase Treatment Does Not Affect Cell Viability. Cells (100,000/well) were incubated with 5 U of TURBO DNase, detached from the plate, and Viacount cell dye was added to differentiate live and dead cells. Cells (1000) were counted by microcytometry in a Guava Technologies PCA 96 instrument.


Conclusions:
Ambions exclusive TURBO DNase is far superior to wild type DNase I in removing unwanted DNA contamination from viable cultured cells. In fact, even at 10 times lower unit co
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