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DNA Removal for Bioprocessing Applications

TURBO DNase Outperforms Wild Type DNase I

Jon Kemppainen & Gary Latham, Ambion, Inc.

TURBO DNase (patent pending) is an engineered version of DNase I that represents a major advance in DNA removal. TURBO DNase binds DNA more tightly than the wild type enzyme, enabling more efficient DNA removal over a broad range of conditions. Recently, Ambion scientists identified a small molecule enhancer of TURBO DNase activity that further magnifies the potency of this enzyme.

As interest in TURBO DNase has grown, Ambion has received a number of inquiries from researchers who require enzymatic DNA removal to enable emerging technologies. For example, several scientists have expressed interest in digesting exogenous DNA from solutions containing viable cells as a requisite bioprocessing step in the manufacturing of next generation clinical products. Current FDA regulations recommend that residual DNA be limited to <10 pg/medicinal dose for therapeutic products. Consequently, efficient DNA removal is an important regulatory and safety requirement. Residual DNA often must be cleared in solutions containing physiological levels of salt (~150 mM) which is required to maintain healthy, living cells. Wild type DNase I maintains only minimal activity in this milieu. TURBO DNase, however, is a salt-tolerant enzyme that functions optimally in
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