Navigation Links
DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

The Trans-Blot SD DNA/RNA agarose gel support frame, used with the Trans-Blot SD semi-dry electrophoretic transfer cell, allows blotting of DNA/RNA from agarose gels to nylon membrane in minutes, without any gel pretreatments. The Trans- Blot SD semi-dry electrophoretic transfer cell generates a high field strength (V/cm2) which facilitates rapid transfers. The blotting frame allows highly efficient transfers by eliminating crushing of agarose gels.

DNA Transfers in 10 Minutes
PCR* fragments, plasmid, and vector DNA ranging in size from several hundred bases to 15 kilobases can now be quickly transferred in as little as 10 minutes. Set-up time is reduced to just minutes because hydrolyzation of DNA fragments is not required prior to transfer.

RNA Transfers in 3035 Minutes
The agarose gel support frame can also be used to transfer RNA up to 3.5 kb in 3035 minutes, although transfer of 28s rRNA is equally efficient as determined by EtBr staining.1

Northern and Southern Blotting
The agarose gel support frame will accommodate 6 mm thick agarose gels up to 15 x 20 cm. Table 1 shows the volumes of agarose required for each gel size.

For convience, 10x TBE and TAE premixed buffers are avaliable. Extra thick, 2.6 mm, filter paper is necessary to provide enough buffer for transfer. The extra thick blot paper must be identical to the dimensions of the agarose gel. Bio-Rads extra thick blot paper is available in four sizes: 7.5 x 10 cm, 10 x 15 cm, 15 x 15 cm, and 15 x 20 cm. Use of any other type of blot paper will decrease transfer efficiency.

1. Lane, K., Zoia, O. and Davidson, J. M., Biotechniques, 12, 3, 340346 (1992).

2. Trans-Blot Instruction Manual.

back to top


Page: All 1 2

Related biology technology :

1. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
2. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
3. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
4. Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae
5. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
6. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
7. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
Post Your Comments:

(Date:11/26/2015)... , England , November 26, 2015 ... Lightpoint Medical, an innovative medical device company specializing in imaging ... grant from the European Commission as part of the Horizon ... the company to carry out a large-scale clinical trial in ... -->      (Logo: , --> ...
(Date:11/25/2015)... , November 25, 2015 2 ... première fois les différences entre les souches bactériennes ... celles des êtres humains . Ces recherches ... et envisager la prise en charge efficace de ... diagnostiqués chez les chats .    --> ...
(Date:11/25/2015)... 25, 2015  Neurocrine Biosciences, Inc. (Nasdaq: NBIX ... and CEO of Neurocrine Biosciences, will be presenting at ... New York . .   ... approximately 5 minutes prior to the presentation to download ... presentation will be available on the website approximately one ...
(Date:11/25/2015)... , Nov. 25, 2015 Orexigen® Therapeutics, Inc. ... participate in a fireside chat discussion at the Piper ... York . The discussion is scheduled for Wednesday, ... .  A replay will be available for ... Stilwell  , Julie NormartVP, Corporate Communications and Business ...
Breaking Biology Technology:
(Date:11/10/2015)... 10, 2015 About signature ... helps to identify and verify the identity of ... as the secure and accurate method of authentication ... particular individual because each individual,s signature is highly ... when dynamic signature of an individual is compared ...
(Date:11/4/2015)... New York , November 4, 2015 ... to a new market report published by Transparency Market ... Share, Growth, Trends and Forecast 2015 - 2022", the global ... of US$ 30.3 bn by 2022. The market is ... the forecast period from 2015 to 2022. Rising security ...
(Date:10/29/2015)... 29, 2015  The J. Craig Venter Institute (JCVI) ... Synthesis and Biosecurity: Lessons Learned and Options for the ... and Human Services guidance for synthetic biology providers has ... --> --> Synthetic biology ... potential to pose unique biosecurity threats. It now is ...
Breaking Biology News(10 mins):