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DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell


The Trans-Blot SD DNA/RNA agarose gel support frame, used with the Trans-Blot SD semi-dry electrophoretic transfer cell, allows blotting of DNA/RNA from agarose gels to nylon membrane in minutes, without any gel pretreatments. The Trans- Blot SD semi-dry electrophoretic transfer cell generates a high field strength (V/cm2) which facilitates rapid transfers. The blotting frame allows highly efficient transfers by eliminating crushing of agarose gels.

DNA Transfers in 10 Minutes
PCR* fragments, plasmid, and vector DNA ranging in size from several hundred bases to 15 kilobases can now be quickly transferred in as little as 10 minutes. Set-up time is reduced to just minutes because hydrolyzation of DNA fragments is not required prior to transfer.

RNA Transfers in 3035 Minutes
The agarose gel support frame can also be used to transfer RNA up to 3.5 kb in 3035 minutes, although transfer of 28s rRNA is equally efficient as determined by EtBr staining.1

Northern and Southern Blotting
The agarose gel support frame will accommodate 6 mm thick agarose gels up to 15 x 20 cm. Table 1 shows the volumes of agarose required for each gel size.

For convience, 10x TBE and TAE premixed buffers are avaliable. Extra thick, 2.6 mm, filter paper is necessary to provide enough buffer for transfer. The extra thick blot paper must be identical to the dimensions of the agarose gel. Bio-Rads extra thick blot paper is available in four sizes: 7.5 x 10 cm, 10 x 15 cm, 15 x 15 cm, and 15 x 20 cm. Use of any other type of blot paper will decrease transfer efficiency.


References
1. Lane, K., Zoia, O. and Davidson, J. M., Biotechniques, 12, 3, 340346 (1992).

2. Trans-Blot Instruction Manual.


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