Navigation Links
DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell


The Trans-Blot SD DNA/RNA agarose gel support frame, used with the Trans-Blot SD semi-dry electrophoretic transfer cell, allows blotting of DNA/RNA from agarose gels to nylon membrane in minutes, without any gel pretreatments. The Trans- Blot SD semi-dry electrophoretic transfer cell generates a high field strength (V/cm2) which facilitates rapid transfers. The blotting frame allows highly efficient transfers by eliminating crushing of agarose gels.

DNA Transfers in 10 Minutes
PCR* fragments, plasmid, and vector DNA ranging in size from several hundred bases to 15 kilobases can now be quickly transferred in as little as 10 minutes. Set-up time is reduced to just minutes because hydrolyzation of DNA fragments is not required prior to transfer.

RNA Transfers in 3035 Minutes
The agarose gel support frame can also be used to transfer RNA up to 3.5 kb in 3035 minutes, although transfer of 28s rRNA is equally efficient as determined by EtBr staining.1

Northern and Southern Blotting
The agarose gel support frame will accommodate 6 mm thick agarose gels up to 15 x 20 cm. Table 1 shows the volumes of agarose required for each gel size.

For convience, 10x TBE and TAE premixed buffers are avaliable. Extra thick, 2.6 mm, filter paper is necessary to provide enough buffer for transfer. The extra thick blot paper must be identical to the dimensions of the agarose gel. Bio-Rads extra thick blot paper is available in four sizes: 7.5 x 10 cm, 10 x 15 cm, 15 x 15 cm, and 15 x 20 cm. Use of any other type of blot paper will decrease transfer efficiency.


References
1. Lane, K., Zoia, O. and Davidson, J. M., Biotechniques, 12, 3, 340346 (1992).

2. Trans-Blot Instruction Manual.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
2. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
3. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
4. Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae
5. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
6. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
7. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... 2016 Houston Methodist Willowbrook Hospital has ... Association to serve as their official health care ... Willowbrook will provide sponsorship support, athletic training services, ... coaches, volunteers, athletes and families. "We ... Association and to bring Houston Methodist quality services ...
(Date:6/23/2016)... -- The Biodesign Challenge (BDC), a university competition that asks ... systems and biotechnology, announced its winning teams at the ... York City . The teams, chosen ... MoMA,s Celeste Bartos Theater during the daylong summit. Keynote ... of architecture and design, and Suzanne Lee , ...
(Date:6/23/2016)... , ... June 23, 2016 , ... STACS DNA Inc., ... Leader at the Arkansas State Crime Laboratory, has joined STACS DNA as a Field ... DNA team,” said Jocelyn Tremblay, President and COO of STACS DNA. “In further expanding ...
(Date:6/23/2016)... 2016  Blueprint Bio, a company dedicated to identifying, ... community, has closed its Series A funding round, according ... "We have received a commitment from Forentis Fund ... to meet our current goals," stated Matthew Nunez ... to complete validation on the current projects in our ...
Breaking Biology Technology:
(Date:6/27/2016)... June 27, 2016 Research and Markets has ... 2016-2020" report to their offering. ... America to grow at a CAGR of 12.28% during ... based on an in-depth market analysis with inputs from industry experts. ... the coming years. The report also includes a discussion of the ...
(Date:6/22/2016)... BETHESDA, Md. , June 22, 2016  The American ... by Trade Show Executive Magazine as one of ... Summit on May 25-27 at the Bellagio in ... based on the highest percentage of growth in each of ... number of exhibiting companies and number of attendees. The 2015 ...
(Date:6/22/2016)... LOS ANGELES , June 22, 2016 ... of identity management and verification solutions, has ... cutting edge software solutions for Visitor Management, ... ® provides products that add functional ... The partnership provides corporations and venues with ...
Breaking Biology News(10 mins):