( d to the volumes of the reagents. With ...)DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

d to the volumes of the reagents. With a sample volume of 2 L, the lower limit corresponds to a DNA concentration of 2.5 ng/mL in the original sample. Lower concentrations probably cannot be measured by this method without pre-concentration or reformulation of the reagents.

SUMMARY
The Lmax microplate luminometer gives a lower limit of 5 pg DNA/well with the DNAQuant DNA quantitation system. These results are as good as, if not better than, results obtained on a standard tube luminometer. The Lmax offers the advantage that the addition of luciferin/luciferase reagent and subsequent read can be automated so that variability due to differences in timing of the flash luminescence reaction can be eliminated.

REFERENCES
1. McGown, E.L. 2000. UV Absorbance Measurements of DNA in Microplates. BioTechniques. 28, 60-64.

2. Gallagher, S.R. 1994. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols In Molecular Biology, Vol. 3. John Wiley & Sons, pp. A3.D.1A3.D3.

3. Sambrook, J., E. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. p. E.5.

4. Molecular Probes, Inc.: www.probes.com.

5. Promega Corporation: www.promega.com

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