Navigation Links
DNA Isolation From 300 to 450 mg Dried or 600 to 1,200 mg Fresh Leaf Tissue


AquaPure Genomic DNA Isolation Kit
Catalog #732-6340
Expected yield range: 90900 g DNA


Method
Cell Lysis
1. Add 300-450 mg dried tissue (finely ground) or 6001,200 mg frozen tissue (which may be finely ground with a mortar and pestle in liquid nitrogen), or 6001,200 mg fresh tissue to a 50 ml tube. Work quickly and keep tissue cold to minimize DNase activity. Note: it may be necessary to vary the amount of starting material depending upon the species, age, tissue preparation, and genome size.

2. Add 18 ml cell lysis solution to the leaf tissue. For dried tissue, cap the tubes and vortex 13 sec to wet the tissue. For unground tissue, homogenize using 3050 strokes with a tube pestle.

3. Incubate cell lysate at 65C for 60 min. After 30 and 60 min, invert capped tube 10 times.

RNase Treatment (Optional)
1. Add 90 l RNase A solution to the cell lysate.

2. Mix the sample by inverting the capped tube 25 times and incubate at 37C for 15 min.

Protein Precipitation
1. Cool sample to room temperature.

2. Add 6 ml protein precipitation solution to the lysate.

3. Cap the samples and vortex vigorously at high speed for 20 sec to mix the protein precipitation solution uniformly with the cell lysate. Alternatively, invert a rack containing the samples 150 times (approximately 2 min) to mix the protein p recipitation solution uniformly with the cell lysate. For species with high polysaccharide content, it may be necessary to incubate sample on ice for 515 min.

4. Centrifuge at 3,000 x g for 10 min. The precipitated proteins should form a tight, green pe
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DNA Isolation From Dried Fresh Leaf Tissue

(Date:7/1/2015)... PARIS , July 1, 2015 ... key developer of biotech crops, today announce a ... generations of insect control traits. "Growers ... the realities of advancing insect resistance. We are ... insect control technology with Genective,s capability in developing ...
(Date:7/1/2015)... ... July 01, 2015 , ... The next-generation sequencing (NGS) clinical market is poised ... molecular diagnostics industry. BCC Research reveals in its new report that the market drivers ... and a growing need for better diagnostics as part of a molecular diagnostics trend. ...
(Date:7/1/2015)... , July 1, 2015   Neuraltus ... the development of groundbreaking drugs to treat neurodegenerative ... today that Robert G. Miller , M.D., Director ... Sutter Health,s California Pacific Medical Center in San Francisco, ... Association for $1.5 million to help fund a ...
(Date:7/1/2015)... July 1, 2015 Isagenix International, a leading ... three Gold, three Silver, and three Bronze Stevie® Awards ... in Chicago this month.The ... business awards program in the U.S. ... strength in developing leaders, cultivating talent, creating a unified ...
Breaking Biology Technology:AgBiome and Genective collaborate to create novel insect-resistant crops 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 3Investigator to Receive $1.5 Million Grant from the ALS Association to Help Fund Confirmatory Phase 2 Study of Neuraltus Pharmaceuticals' NP001 2Investigator to Receive $1.5 Million Grant from the ALS Association to Help Fund Confirmatory Phase 2 Study of Neuraltus Pharmaceuticals' NP001 3Investigator to Receive $1.5 Million Grant from the ALS Association to Help Fund Confirmatory Phase 2 Study of Neuraltus Pharmaceuticals' NP001 4Isagenix Earns Nine American Business Awards 2Isagenix Earns Nine American Business Awards 3
... , , , , Introduction ... , , , , , ... is an important part of your application. Many variables must be considered, , ... evaporation, and accessory chiller—so it's no surprise to find more , ...
... , , , Katherine A. Borkovich ... University of Texas-Houston Medical Center, , , ... Kimberly Vollmer, Brinkmann™ Instruments Inc., BioSystems Application , ... , , , INTRODUCTION: , ...
... , , , , ... due to a hypoosmolar buffer system and short pulses in the s-range. , ... of sample resistance (patent pending)., , , ... , Two cell lines (mouse fibroblast cells, human T-lymphocytes) ...
Cached Biology Technology:Optimizing Büchi® Rotary Evaporator Applications 2Optimizing Büchi® Rotary Evaporator Applications 3Optimizing Büchi® Rotary Evaporator Applications 4Optimizing Büchi® Rotary Evaporator Applications 5Optimizing Büchi® Rotary Evaporator Applications 6Optimizing Büchi® Rotary Evaporator Applications 7Optimizing Büchi® Rotary Evaporator Applications 8Efficient Transfection of Neurospora Crassa 2Efficient Transfection of Neurospora Crassa 3Efficient Transfection of Neurospora Crassa 4Efficient Transfection of Neurospora Crassa 5
(Date:6/24/2015)... This report provides market analysis of the ... It contains an analysis of the drivers, challenges, and ... from the short, medium, and long term perspective landscapes. ... and technology trends that currently, prevail in the market. ... to efficiently manage the increasing marine traffic and increasing ...
(Date:6/24/2015)... Research and Markets( ... "Huawei Ascend Mate 7 Fingerprint Sensor - ... Huawei,s technological choice for the fingerprint ... Apple,s and Samsung,s one. The Ascend Mate 7 ... Cards, a main actor in capacitive sensing human ...
(Date:6/23/2015)... Minn. , June 23, 2015   ... company that supports the entire spectrum of clinical ... iMedNet ™ , the company,s intuitive, ... been awarded a Silver 2015 Stevie® Award by ... Health Products & Services Website category.  The American ...
Breaking Biology News(10 mins):Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2Huawei Ascend Mate 7 Fingerprint Sensor - Reverse Costing Analysis 2MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2
... second generation biofuels made from inedible plant material, ... and numerous scientific studies have shown them to ... our transportation needs. However, before we can begin ... grassoline, numerous barriers must be overcome, starting with ...
... has pledged $50,000 to fund the work of University ... who are working to develop a computer program that ... Wii video game console. Students James McKee, Jack ... the project as seniors at UAB. Along with faculty ...
... A new unique work published by Springer, the ... the influence of complexity and system science to a ... In eleven volumes, available in print and electronically, ... applying the concepts of complexity theory together with the ...
Cached Biology News:New technique can fast-track better ionic liquids for biomass pre-treatments 2New technique can fast-track better ionic liquids for biomass pre-treatments 3New technique can fast-track better ionic liquids for biomass pre-treatments 4UAB students' Nintendo Wii CPR earns American Heart Association support 2A complete view of complexity in science and society in a new authoritative reference work 2
RABBIT ANTI QUINOLINIC ACID...
... Ferrofluid products perform optimally when ... Systems MagCellect Magnet. To accommodate ... iron boron permanent magnet holds ... mm test tubes or two ...
Form: Concentrated Applications: ELISA...
Form: Ready to use Applications: ELISA...
Biology Products: