Navigation Links
DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging

Introduction

Oxidative stress, radiation and other external insults have been shown to damage DNA molecules in cells derived from organisms as diverse as bacteria, yeast, drosophila, rodents and man (Friedberg et al. 2005). The presence of DNA damage may lead to cell cycle checkpoint arrest to allow time for DNA repair processes to occur. If however, the system becomes overwhelmed or the DNA repair mechanisms are impaired, the cell either enters the apoptosis pathway or become cancerous due to the accumulation of mutations resulting from replication of damaged DNA. Therefore, a complete understanding of DNA repair mechanisms is of great interest in the study of cancer prevention and treatment. In addition, this process has been implicated in cellular senescence and aging (Sedelnikova et al. 2004). Following induction of DNA double strand breaks, the specialized histone protein H2AX becomes phosphorylated and rapidly (within minutes) accumulates at the sites of DNA damage forming distinct foci (Paull et al. 2000).

H2AX foci formation is followed by recruitment of many other proteins involved in the DNA repair process including the p53 binding protein 53BP1 (Schulz et al ., 2000). Over long time periods (hours), the number of foci declines with the progression of DNA repair. Detection and quantification of foci development as an indicator of DNA damage and repair is of great interest to groups investigating these pathways (Kim et al . 2005). The assay is generally performed using coverslips and chamber slides and imaged on fluorescence microscopes (Sedelnikova et al . 2004). The demands for high spatial resolution and sophisticated image analysis, however, have hindered the transfer of this assay into a multi-well plate format and thus into the drug screening arena. Our goal was to establish image acquisition and analysis methods allowing quantification of foci develop
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
2. Quantification made easy
3. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
4. Quantification of siRNA Silencing Efficiency Using the LightCycler System
5. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
6. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
7. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:8/21/2014)... 2014 Telomere Biosciences, LLC (Telomere Biosciences) ... for Dogs" with Telomerase Activation Complex , ... world for dogs. Telomeres are the protective caps ... body. A wealth of ground-breaking research by some ... and Aging, including Nobel laureates, has demonstrated a ...
(Date:8/21/2014)... , Aug. 21, 2014  Decision Resources ... Brazil and Mexico ... uptake of several newly approved agents, the anticipated ... indication and the launch of emerging biologics will ... In particular, the emerging IL-5 inhibitors will introduce ...
(Date:8/21/2014)... , Aug. 21, 2014 OTC ... Pharmaceuticals Inc., a biotechnology company, on its approval ... previously traded on OTCQX®, the best marketplace for ... Markets Group. Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO ... the successful execution of its growth strategy and ...
(Date:8/21/2014)... Aug. 21, 2014  Four-dimensional (4-D) printing develops ... on external stimuli such as changes in temperature. ... disruptive effect in multiple industries.  In the near ... by printing objects ranging from human organs to ... Logo - http://photos.prnewswire.com/prnh/20140820/138289 ...
Breaking Biology Technology:Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 3Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 2Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 3Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 4
... there was a monopoly phone service prior to the 1984 ... exclusivity in the territory and we will provide universal service ... a geographic subscriber base, the traditional phone companies ensured that ... fair price. There was one price across the board. That ...
... - Does the Wisconsin Alumni Research Foundation's ... for approval? , ,The foundation's stem cell patent is ... it is overly broad and is suppressing scientific research. ... the more than 300 academic licenses it has issued ...
... to clarify the definition of records for the treatment ... incorporate them into Electronic Health Records, has been signed ... 650 creates an exception to current law pertaining to ... to mental health records when they are treating patients ...
Cached Biology Technology:Does redefining universal service help bridge the digital divide? 2Does redefining universal service help bridge the digital divide? 3Does redefining universal service help bridge the digital divide? 4Does redefining universal service help bridge the digital divide? 5WARF stem cell patent faces long and winding road 2WARF stem cell patent faces long and winding road 3WARF stem cell patent faces long and winding road 4WARF stem cell patent faces long and winding road 5
(Date:8/22/2014)... a leading source of information for businesses and professionals, ... Riverside in its 2014 list of "some of the ... generate the list, the company analyzed citation data over ... has had enormous impact. , The following UC Riverside ... the greatest number of highly cited papers in one ...
(Date:8/22/2014)... NAIROBI (22 August 2014)With ... as a major threat to food security across the continent, ... five years, 1.7 million African farmers in 13 countries have ... helped them double or even triple crop yields. , The ... (AGRA) focuses on intensive efforts initiated five years ago to ...
(Date:8/22/2014)... FASEB MARC (Maximizing Access to Research Careers) Program ... Grant Writing Seminar & Practical Exercises Workshop which will ... Maryland from August 25-26, 2014. These awards are ... research scientists from underrepresented groups in the the FASEB ... year MARC conferred 11 awards totaling $20,350. , The ...
Breaking Biology News(10 mins):5 UC Riverside scientists among world's most influential scientific minds 2Effort to confront Africa's soil health crisis helps millions of farmers triple yields 2Effort to confront Africa's soil health crisis helps millions of farmers triple yields 3Effort to confront Africa's soil health crisis helps millions of farmers triple yields 4
... next generation sequencing (NGS) and Certified Service Provider for ... a leading company in complex analysis and interpretation of ... complete human exome array, based on Roche NimbleGen sequence ... comprehensive service for human exome capture, targeted re-sequencing and ...
... be used to "see" something as small as an ... has once again overturned conventional wisdom. Secretary of Energy, ... National Laboratory (Berkeley Lab) Steven Chu led the development ... microscopy to ,image objects or the distance between them ...
... of all acute leukemias represents a small population of ... standard treatments available today. Though current chemotherapy can produce ... of experiencing a recurrence and dying from the disease. ... rare form of leukemia called acute promyelocytic leukemia (APL), ...
Cached Biology News:Eurofins MWG Operon and Genomatix developed most complete human exome array 2Correcting a trick of the light brings molecules into view 2Correcting a trick of the light brings molecules into view 3Correcting a trick of the light brings molecules into view 4Grant to fund research into eradicating the leukemia stem cell 2
Determin interaction of your protein with Echelons lipids...
Goat Anti-Horse IgG(T) Antibody, Unconjugated...
PA coated Agarose beads LPA Beads...
... PAX3 This protein is a member ... transcription factors. Members of the PAX family ... a paired-type homeodomain. These proteins play critical ... box gene 3 are associated with Waardenburg ...
Biology Products: