Navigation Links
DGGE Analysis for Non-Invasive Studies of Primate Diet

Jodi A. Irwin, Department of Cell and Molecular Biology, Northwestern University Medical School, 303 E. Chicago Ave., Chicago, Il 60611-3008 j-irwin@nwu.edu; Cristin Orrego, Conservation Genetics Laboratory, Department of Biology, 1600 Holloway Avenue, San Francisco State University, San Francisco, CA 94132 cob@sfsu.edu


Introduction

The applications of denaturing gradient gel electrophoresis (DGGE) to the investigation and diagnosis of human genetic diseases are well-known (Fodde and Losekoot 1994). More recently, however, DGGE has also become a valuable tool for addressing questions pertaining to natural history. It has been applied to studies of population genetics (Lessa and Applebaum 1993) and phylogeography (Lessa 1992), and it has been particularly useful in analyses of microbial communities (Muyzer et al. 1993, Murray et al. 1996).

We demonstrate the use of DGGE as a molecular technique that can be employed in the absence of behavioral data for examining the diet of free-ranging howler monkeys (Alouatta pigra) (Figure 1). The difficulties associated with the collection of behavioral data for diet studies are well known. These include problems related to observing monkeys through thick foliage, the large distances separating observer from subject, and the difficulty of identifying tree species with very similar leaf morphologies. In order to circumvent these problems, we investigated the potential of a polymerase chain reaction* (PCR)/DGGE method for augmenting knowledge of the howler diet. We hope that the development of a non-invasive technique for examining diet will facilitate studies of wild populations, particularly those for which behavioral data are not available
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DGGE Analysis for Non Invasive Studies Primate Diet

(Date:1/14/2014)... Boston, MA (PRWEB) January 14, 2014 iLab ... Michelle Detwiler as the new Director of Product Strategy. In ... as well as iLab sub-teams to guide in the development ... that iLab provides the maximum possible benefit to the scientific ...
(Date:1/14/2014)... January 14, 2014 Global Record ... innovative information technology solutions for patients, physicians, the ... stakeholders, announced today the signing of a three-year ... and Drug Administration (FDA). This initiative is ...
(Date:1/14/2014)... Kerr Corporation, a leading manufacturer of innovative dental ... about dual arch impressions on its dental blog. ... blog entry serves up a list of tips to help ... by Dr. David Little as he crafts a porcelain crown ...
(Date:1/14/2014)... BioMedomics, Inc. , a Point of Care ... novel disease specific POC tests, announced today that it has ... of $690,000. The investment is from private investors and is ... of private investors has significant successful experience in early investments ...
Breaking Biology Technology:iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2BioMedomics, Inc. Closes $690K Round of Financing 2
... , - Covance experts to present best practices for improving ... , PRINCETON, N.J., June 16 Covance Inc. is pleased ... at the Drug Information Association,s (DIA) 45th Annual ... Calif. , , The DIA Annual ...
... Relationship increases access to testing for sleep-related illnesses , ... a California-based provider of in-home sleep testing and Continuous ... with Aetna, one of the nation,s largest healthcare benefit ... will now be able to serve more patients with ...
... , , , PHILADELPHIA, June 16 ... as Pew Latin American Fellows in the Biomedical Sciences. The ... level scientists to work with leading researchers in the United ... $35,000 to purchase essential equipment to continue their research in ...
Cached Biology Technology:Covance to Present at the Drug Information Association's 45th Annual Meeting 2Covance to Present at the Drug Information Association's 45th Annual Meeting 3Covance to Present at the Drug Information Association's 45th Annual Meeting 4Covance to Present at the Drug Information Association's 45th Annual Meeting 5SleepQuest Signs National Contract With Aetna 2Ten Top Latin American Scientists Named 2009 Pew Fellows in the Biomedical Sciences 2Ten Top Latin American Scientists Named 2009 Pew Fellows in the Biomedical Sciences 3
(Date:4/17/2014)... NY, April 17, 2014The development of stem cell therapies ... ability to characterize stem cell populations based on cell ... discovered a new marker that is highly expressed in ... cord blood, which they describe in an article in ... Mary Ann Liebert, Inc., publishers. The article is available ...
(Date:4/17/2014)... N.M. A credit-card-sized anthrax detection cartridge developed at ... business makes testing safer, easier, faster and cheaper. ... anthrax, is commonly found in soils all over the ... in both humans and animals. The bacteria can survive ... B. anthracis may occur through skin contact, ...
(Date:4/17/2014)... need to produce hydrogen sulfide in order to properly ... study from the Center for Craniofacial Molecular Biology at ... Professor Songtao Shi, principal investigator on the project, said ... governs the flow of calcium ions. The essential ions ... osteogenesis, or the creation of new bone tissue, and ...
Breaking Biology News(10 mins):Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3
... 2011 -- Scientists from the University of Miami (UM) Rosenstiel ... national research team to find two plumes of oil-based pollutants ... a study published in this week,s issue of the journal ... the mechanism by which the crude oil traveled from the ...
... As evidence regarding the health benefits of consuming ... increasing debate about which cocoa and chocolate products deliver ... steps in cocoa and chocolate production diminish the levels ... published paper, scientists reported on the effect of conventional ...
... in French . The Universit de ... the Botanical Garden. The new Centre, the result of a ... Montral Insectarium, two Espace pour la vie (Montral Space for ... its preservation and its promotion. The Centre will not only ...
Cached Biology News:New study reveals aerosol plumes downwind of the Deepwater Horizon oil spill 2Study clarifies the role of cocoa bean handling on flavanol levels 2The University of Montreal Biodiversity Centre opens 2The University of Montreal Biodiversity Centre opens 3
... Safe photographic filter (S37100, Photographic Filters ... is ideal for black-and-white photography of ... DNA gel stain, the safer ethidium ... Safe photographic filter is identical to ...
... Sensitivity Freezing Point Osmometer for 10 ... volume applications, including pediatric, neonatal, geriatric, ... Automatic Calibration with 2 or ... printer, keypad and software for statistical ...
...
Ultra Pure Grade. Used in conjunction with IPTG to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the presence of be...
Biology Products: