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DGGE Analysis Using the DCode System, Rev B

o distinguish between samples that are mutant or wild type. From the results, it was possible to detect single base substitutions in the mutant samples from the wild-type sample using parallel DGGE.

Parallel DGGE can be used as a method for screening mutations in the -globin gene using the DCode system. The DCode system can precisely control the buffer temperature due to a large buffer volume, electronic heat control, a stirrer, and a circulating pump.


Reference
Fischer SG and Lerman LS, DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory, Proc Natl Acad Sci USA 80, 15791583 (1983)

The polymerase chain reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche. Use of the PCR process requires a license.


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