Navigation Links
Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives ,,, in the yeast Saccharomyces cerevisiae: Detection in vivo using ,,, the Varian Cary Eclipse

ic fluorescent protein (See the Clontech Web Site3 for a comprehensive list of excitation/emission maxima of fluorescent proteins.) Emission scans were recorded for GFP, BFP, CFP, YFP and DsRed. Operating parameters for scans of DsRed are given in Figure 2.

Results

Emission spectra for each of the fluorescent proteins are shown in Figure 3.

Discussion

Spectra from all fluorescent proteins expressed in the cytosol of the yeast cells were easily recorded and correspond to expected characteristics. Noise produced by cellular autofluorescence and scatter was negligible, mainly due to internal filters fitted to both excitation and emission monochromators on the Varian Cary Eclipse. Reducing autofluorescence (from either sample of interest or media) is of paramount importance when using fluorescent proteins that have either low fluorescent intensity, or are excited by high energy UV light (such as BFP). UV light initiates a strong fluorescent response from many components of the cellular environment and must be minimized to prevent masking of emission from target fluorescent proteins, especially when subtle protein interactions are being monitored7.

Conclusion

The Varian Cary Eclipse with multicell and temperature control accessories provides a simple, rapid and reproducible assembly with which to measure and characterise different fluorescent proteins expressed in whole cells. The opportunity now exists to specifically tag proteins of interest within cells using different GFPs and monitor interactions simultaneously using multiwavelength readings which allow automated excitation and
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
3. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
4. Successful stabilization of gene expression profiles
5. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
6. QuantiTect SYBR Green RT-PCR Kit
7. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
8. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
9. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
10. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
11. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
Post Your Comments:
(Date:8/3/2015)... ... 2015 , ... Sterlitech Corporation introduces the compact, quiet, and ... laboratory equipment. With optimized features, such as PTFE-coated contact surfaces, an oil-free design, ... solution for a broad range of vacuum filtration applications. , Featuring a 1/7 ...
(Date:7/31/2015)... China Cord Blood Corporation (NYSE: CO) ("CCBC" ... provider of cord blood collection, laboratory testing, hematopoietic stem ... that the Company has filed its Annual Report on ... The filed Form 20-F includes audited financial statements for ... 20-F can be accessed by visiting the U.S. Securities ...
(Date:7/30/2015)... July 30, 2015 AACC welcomed thousands ... 2015 AACC Annual Meeting & Clinical Lab Expo ... 26–30. The meeting showcased revolutionary advancements in clinical ... of healthcare providers to diagnose patients quickly and ... treatment. As of Wednesday, July ...
(Date:7/30/2015)... ... July 30, 2015 , ... The 2015 Market Research ... on the current state of the global Propanol market with a focus on the ... Eastman, Dow, Carboclor, Mitsui Chemicals, Oxea, ISU Chemical, Sanofi, Royal Dutch Shell, LG Chem ...
Breaking Biology Technology:Sterlitech Launches New Line of Vacuum Pumps 2China Cord Blood Corporation Files Its Annual Report on Form 20-F 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4Global Propanol Market Technical Data and Manufacturing Analysis 2020 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 3
... Lilly and Company (NYSE: LLY ) announced its ... has a 25-year history of success with laws promoting the ... process under which generic versions of those new medicines can ... take that step with the most advanced new medicines, called ...
... International and C,elle(SM), Welcomes President Obama,s Stem Cell Decision ... Cryo-Cell International, the nation,s most established private family cord ... in the stem cell and cord blood industry since ... taken place over the past decade and is pleased ...
... Healthcare institutions often struggle with the numerous and ... expensive fines, and prevent an adverse Joint Commission ... (EH&E) has designed an automated management solution for ... Compliance Calendar is a customized, web-based management resource ...
Cached Biology Technology:Pathway for Biosimilars Act Protects Patients, Promotes Competition, Preserves Innovation and Creates Quality Jobs, Lilly Says 2Pathway for Biosimilars Act Protects Patients, Promotes Competition, Preserves Innovation and Creates Quality Jobs, Lilly Says 3Stem Cell Industry Insider Mercedes Walton Discusses President Obama's Stem Cell Executive Order 2Stem Cell Industry Insider Mercedes Walton Discusses President Obama's Stem Cell Executive Order 3Healthcare Institutions Can Now Stay Confidently Up-to-Date With New Regulatory Compliance Calendar 2Healthcare Institutions Can Now Stay Confidently Up-to-Date With New Regulatory Compliance Calendar 3
(Date:7/31/2015)... de julho de 2015 A 10 a. ... será realizada pela BGI de 22-25 de outubro de 2015, ... A conferência está celebrando seu 10 o. aniversário ... se tornou uma das reuniões mais influentes do mundo ... mais dinâmicos, entusiastas e prazerosos. A ...
(Date:7/31/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... growing mobile commerce market and creator of the Wocket® ... 62/198989 for ELECTRONIC CRYPTO-CURRENCY MANAGEMENT METHOD AND ... advance crypto-currencies such as Bitcoin into the consumer market ... to manage all payments.  The technology ...
(Date:7/27/2015)... 2015   Zynx Health ™, the market ... solutions, today announced that its ZynxCarebook ™ ... Android smartphones and tablets. With this expansion, care ... use ZynxCarebook to securely exchange messages and share ... transitions to other care settings, and improve clinical ...
Breaking Biology News(10 mins):A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3NXT-ID Patents Electronic Crypto-Currency Management Technology 2NXT-ID Patents Electronic Crypto-Currency Management Technology 3NXT-ID Patents Electronic Crypto-Currency Management Technology 4Zynx Health Adds Android Device Support to ZynxCarebook Solution 2Zynx Health Adds Android Device Support to ZynxCarebook Solution 3
... Brian Chen has been awarded a prestigious Sloan Research ... Formation, investigates a big subject: deciphering the assembly instructions ... neural circuits wire up with precision. One of the ... the correct synaptic contacts during its development when faced ...
... named Brontomerus mcintoshi , or "thunder-thighs" after its enormously powerful ... is described in a paper recently published in the journal ... the U.K. and the U.S. A member of the ... Brachiosaurus , Brontomerus may have used its powerful ...
... the crowd? Is that all I,ll ever be? ... Do you ... the Crowd" It may seem paradoxical, but being ... UCLA life scientists. Biologists Kimberly Pollard and Daniel ... alarm-call vocalizations in eight species of rodents that live in social ...
Cached Biology News:Prestigious Sloan Fellowship awarded 2New 'thunder-thighs' dinosaur discovered 2New 'thunder-thighs' dinosaur discovered 3Even in a crowd, you remain unique, UCLA life scientists report 2
Request Info...
Request Info...
... 25 g Blocking Reagent , 2 x 50 ml ... Buffer , 0.2 ml BL21 glycerol stock , ... glycerol stock , 1010 pfu T7Select Positive Control Lysate ... 50 l S-protein HRP Conjugate , 2 ml T7Select ...
Attoglow Western Blot Enhancing Kit contains Millennium Enhancer which can significantly boost Western blot signals, and is compatible with other enhancing mechanisms to achieve multiplied enhancing ...
Biology Products: