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Cyclodextrin enhances room temperature phosphorescence

nts, the phosphorescence intensity of the included PAH was found to depend on the concentration of the heavy-atom moiety and on the concentration of CD. Figure 4 illustrates the relationship of the phosphorescence intensity of phenanthrene to the concentration of DBE. As long as the concentration of DBE is low with respect to that of CD, DBE molecules added into the solution are incorporated in the trimolecular inclusion complex, causing a sharp increase in phosphorescence intensity. When the DBE concentration reaches that of the CD, so that complexation is virtually complete, further addition of DBE enhances the phosphorescence only slightly.


Figure 5 demonstrates the dependence of phosphorescence intensity on the concentration of CD. The S-shaped curve traces the phosphorescence intensity of the phenanthrene-DBE solution as β-cyclodextrin is added. The initial steep slope has been attributed to the sharply-increasing probability that a phenanthrene molecule exiting the cavity of a CD-DBE molecule will find another CD-DBE molecule in close proximity.4


Conclusion
Cyclodextrin-enhanced room-temperature phosphorescence shows promise as a sensitive analytical technique. Sample preparation is simple, data-acquisition and processing are easily automated with a FLUOROLOG spectrometer, and the result is an intense, well-structured RTP signal. CD-RTP offers the advantages of increased selectivity based on molecular geometry, and partial immunity to quenching by dissolved O2 molecules.


Acknowledgement
The Fluorescence Group would like to thank Dr. L.J. Cline Love and Stephen Scypinski for their generous cooperation in the preparation of this technical note. We would also like to acknowledge permission from the American Chemical Society to reprint figures originally published in
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