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Custom and library siRNA for efficient gene silencing

The QIAGEN Multiplex PCR Kit has been successfully used for typing and analysis of transgenic animals and plants, amplification of multiple DNA regions for SNP analysis, and typing and detection of bacteria and viruses. A special protocol for amplification and analysis of microsatellites is also provided. In addition, the kit contains a protocol for multiplex amplification using Q-Solution, suitable for targets with high GC content or complex secondary structure.

Microsatellite Analysis with Optimized QIAGEN Protocol
Figure 2 Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and Penta E was carried out using 1 ng of K562 human genomic DNA and fluorescein-labeled primers. Reactions were analyzed on the ABI PRISM 377 Sequencer. Top: High sensitivity and uniform signal intensity using the QIAGEN Multiplex PCR Kit and the microsatellite protocol included in the QIAGEN Multiplex PCR Handbook. Bottom: Results using a hot-start DNA polymerase from Supplier AII.

Easy to use

The QIAGEN Multiplex PCR Kit contains a master mix specifically designed for multiplex PCR applications. The master-mix format makes handling and reaction setup both fast and easy. Use of a master-mix format reduces time and handling for reaction setup and increases reproducibility by eliminating many possible sources of pipetting errors. The master mix is stable at 20C and 4C for many months. Storage at 4C eliminates thawing time, providing even faster setup of multiplex PCR assays. Reaction setup at room temperature also makes the QIAGEN Multiplex PCR Kit the ideal tool for automated PCR setup.

The easy-to-follow protocol and the robust universal multipl
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