Navigation Links
Custom and library siRNA for efficient gene silencing

bles a highly stringent hot start and allows reaction setup at room temperature without nonspecific artifacts. The newly developed multiplex PCR buffer in the master mix further enhances the sensitivity of the multiplex reaction. This innovative, new formulation was specifically developed and optimized for multiplex PCR. In contrast to conventional PCR reagents, the new buffer contains a balanced combination of salts and additives to ensure comparable efficiencies for annealing and extension of all primers in the reaction (Figure 1).

Successful 16plex PCR Using the QIAGEN Multiplex PCR Kit

Figure 1 Multiplex PCR of 16 targets (99C955 bp) was carried out for 35 cycles using standard conditions (Std) for the QIAGEN Multiplex PCR Kit (Q) without further optimization or using a variety of conditions with a hot-start DNA polymerase from Supplier AII (AII). A Comparison using 2.5 units/50 l reaction of the hot-start DNA polymerase from Supplier AII and with the indicated Mg2+ concentrations. B Comparison using the optimized Mg2+ concentration (3.5 mM) for the hot-start DNA polymerase from Supplier AII and the indicated amounts of enzyme per 50 l reaction. M: markers.

Versatile for many applications

The QIAGEN Multiplex PCR Kit is well suited for many types of multiplex PCR applications using different detection methods. PCR products can be visualized on agarose gels or detected using fluorescently labeled primers. Detection of fluorescently labeled products can be performed on automatic gel-based DNA sequencers (e.g., the ABI PRISM 377 DNA Sequencer; Figure 2), as well as those based on capillary electrophoresis (e.g., the ABI PRISM 310 Genetic Analyzer).
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom siRNA Oligo Synthesis Service
3. Ambion Custom
4. Ambion Custom: Made-to-Order
5. Custom RNA & RNA Isolation Services to Meet Your Specific Needs
6. Cancer siRNA Oligo Set Version 1.0
7. Library siRNA
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Quantification of siRNA Silencing Efficiency Using the LightCycler System
10. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
11. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Custom and library siRNA for efficient gene silencing

(Date:3/25/2015)... (PRWEB) March 25, 2015 Data ... Novel Mechanism of Action in Neuromuscular Diseases ... – Cytokinetics, Incorporated (Nasdaq: CYTK) announced the publication ... muscle troponin activator tirasemtiv in the journal Neurotherapeutics. ... IIa “Evidence of Effect” or hypothesis-generating clinical trial ...
(Date:3/25/2015)... the HAGUE, Netherlands , March 25, 2015 /PRNewswire/ ... therapy for chronic gastro-esophageal reflux disease (GERD) in the ... Surgery . 2015; 157(3):556-567 ], establishing the long ... The study included 25 patients with severe ... taking prescribed daily proton pump inhibitor (PPI) medication for ...
(Date:3/25/2015)... 25, 2015  18 piglets born recently are ... by scientists in the College of Agriculture and Natural ... breakthrough in the field of genetic engineering. Bhanu ... Animal & Avian Sciences (ANSC) and Ki-Eun Park, ... produced genome-edited pigs using a recently developed, groundbreaking ...
(Date:3/25/2015)...  CASI Pharmaceuticals, Inc. (Nasdaq: CASI ), a ... of innovative therapeutics addressing cancer and other unmet medical ... on China , announces that it ... therapy drug candidate ENMD-2076 in triple-negative breast cancer (TNBC) ... Sciences in Beijing , China.  ...
Breaking Biology Technology:Cytokinetics Announces Publication of Results from Phase II Trial of Tirasemtiv in Patients with Myasthenia Gravis 2Cytokinetics Announces Publication of Results from Phase II Trial of Tirasemtiv in Patients with Myasthenia Gravis 3Cytokinetics Announces Publication of Results from Phase II Trial of Tirasemtiv in Patients with Myasthenia Gravis 4Cytokinetics Announces Publication of Results from Phase II Trial of Tirasemtiv in Patients with Myasthenia Gravis 5Cytokinetics Announces Publication of Results from Phase II Trial of Tirasemtiv in Patients with Myasthenia Gravis 6EndoStim LES Stimulation therapy demonstrates long term safety and efficacy results in treating reflux patients 2EndoStim LES Stimulation therapy demonstrates long term safety and efficacy results in treating reflux patients 3University of Maryland Researchers Successfully Produce Genome-edited Pigs Using Revolutionary Technology 2CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 2CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 3CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 4CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 5
... June 24, 2011 /PRNewswire-Asia-FirstCall/ -- China Agri-Business, Inc. ... manufacturer and distributor of organic agricultural application products ... its Chinese operating subsidiary, Shaanxi Xinsheng Centennial Agriculture ... right to manage and operate Shaanxi Qinfeng Agrochemical ...
... - U.S. Department of Energy researchers have won 36 ... R&D Magazine for the most outstanding technology developments with ... annually in recognition of exceptional new products, processes, materials ... the market the previous year. "I want to ...
... 2011 BioNeutral Group Inc., (OTCBB: BONU.ob) today ... distribution phase of their revolutionary Cold Sterilant and ... BioNeutral was selected by New Jersey based T.U.C.S. ... service and distribution company, because of the superior ...
Cached Biology Technology:China Agri-Business Acquired Management and Operation Rights of Shaanxi Qinfeng Agrochemical Inc. in Xi'an, China 2China Agri-Business Acquired Management and Operation Rights of Shaanxi Qinfeng Agrochemical Inc. in Xi'an, China 3China Agri-Business Acquired Management and Operation Rights of Shaanxi Qinfeng Agrochemical Inc. in Xi'an, China 4Department of Energy projects win 36 R&D100 Awards for 2011 2Department of Energy projects win 36 R&D100 Awards for 2011 3Department of Energy projects win 36 R&D100 Awards for 2011 4Department of Energy projects win 36 R&D100 Awards for 2011 5Department of Energy projects win 36 R&D100 Awards for 2011 6Department of Energy projects win 36 R&D100 Awards for 2011 7Department of Energy projects win 36 R&D100 Awards for 2011 8Department of Energy projects win 36 R&D100 Awards for 2011 9BioNeutral Starts Commercial Deployment of Its Ygiene 206 Sterilant and Ogiene Products Within Port Authority Facilities Using T.U.C.S. Cleaning Services 2BioNeutral Starts Commercial Deployment of Its Ygiene 206 Sterilant and Ogiene Products Within Port Authority Facilities Using T.U.C.S. Cleaning Services 3BioNeutral Starts Commercial Deployment of Its Ygiene 206 Sterilant and Ogiene Products Within Port Authority Facilities Using T.U.C.S. Cleaning Services 4
(Date:3/11/2015)... , March 11, 2015   The ... its platform to scientifically measure and harness music ... to real-time biometrics and objective measurements of physiology, ... music at scale in large populations. It is ... technologists and clinicians perform rigorous studies and accelerate ...
(Date:3/10/2015)... OXFORD, Conn. , March 10, 2015 /PRNewswire/ ... NXTDW) ("NXT-ID" or the "Company"), a biometric authentication ... reports the company,s Wocket™ smart wallet has been ... 2015" by Rethink Modern. Rethink Modern ... sharing modern and innovative items for your home and lifestyle that ...
(Date:3/2/2015)... 2, 2015 Synaptics Inc. (NASDAQ: ... interface solutions, today announced a new implementation of ... enable rapid and secure transactions and reliable identification ... is a turnkey solution providing mouse and keyboard ... integrate fingerprint ID solutions into their product offerings, ...
Breaking Biology News(10 mins):Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 2Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 4Synaptics Unveils New Fingerprint ID Solution for Gamers 2Synaptics Unveils New Fingerprint ID Solution for Gamers 3
... carbon per year from wind erosion and dust storms affecting ... research.,Top soil is rich in nutrients and carbon but is ... Dawn, in Sydney in 2009. When wind lifts ... location of soil carbon. Some carbon falls ...
... Bowman, climate science communications expert and host of the Climate ... Peter Wadhams, two of the three authors of an article ... controversy since its release on July 24, 2013. The authors ... warming in the Arctic the release of methane from ...
... For a new study in the Journal of ... lipids affect growth performance in weaned pigs. The researchers ... According to the researchers, it is important to ... have improved over the years. Dr. Olayiwola Adeola, ...
Cached Biology News:Soil carbon 'blowing in the wind' 2Researchers re-evaluate swine nutrition 2
...
recombination at attR1 and attR2 sites...
... Peroxidase Conjugate, 2 ml. ... enzyme molecules for use ... situ hybridization applications.High specificity ... Labeling & Detection, Protein ...
Whole rabbit blood (any breed, either sex) is collected at the time of slaughter and processed immediately at 4C, bottled, frozen and stored at -70C....
Biology Products: