( setup for fast and reproducibl...)Custom and library siRNA for efficient gene silencing

setup for fast and reproducible results

No optimization required

Establishing multiplex PCR assays is a tedious and time-consuming procedure. In most cases, not all PCR products are generated, and nonspecific background products, such as primer-dimers, are often observed. To get acceptable results requires extensive optimization of the amounts of Taq DNA polymerase, Mg2+, additional reagents, and primers. Often, cycling parameters or reaction buffer formulations need to be changed. In many cases, the results are still disappointing, requiring further extensive optimization (see flowchart). The QIAGEN Multiplex PCR Kit eliminates the need for optimization, making the development of multiplex PCR assays both simple and fast. The master mix contains pre-optimized concentrations of HotStarTaq DNA Polymerase and MgCl2, plus dNTPs and a PCR buffer newly developed for multiplex reactions. Excellent results can be achieved the first time using fixed primer concentrations and the standard multiplex PCR cycling protocol, included in the QIAGEN Multiplex PCR Handbook. Typically, there is no need to adjust reaction parameters (see flowchart).

Multiplex PCR Setup

High specificity and sensitivity

Nonspecific PCR products and primer-dimers are often generated during multiplex PCR due to the large amount of different primers in the reaction. Amplification of these nonspecific products competes with amplification of the desired PCR products, leading to decreased sensitivity. With the QIAGEN Multiplex PCR Kit, specificity is significantly increased by use of HotStarTaq DNA Polymerase in the master mix. This enzyme ena
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