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Custom and library siRNA for efficient gene silencing

Figure 1 Western blot of extracts from transfected HeLa-S3 cells probed with lamin A/C monoclonal primary
antibodies. Cells were transfected using the indicated reagents. siRNA: short interfering RNA duplex targeting
lamin A/C at position 608630 relative to the start codon; TM: TransMessenger Transfection Reagent; ODN:
Scrambled 24mer oligodeoxynucleotide (control). Efficient, Targeted Gene Silencing Figure 2 Relative expression levels of lamin A/C after siRNA transfection. The value for the mock transfection was set as 1. Data were normalized to the expression level of GAPDH (internal control), which was quantified
by real-time RT-PCR using OmniscriptReverse Transcriptase and the QuantiTect SYBR Green PCR Kit. Expression levels were calculated using a standard curve generated using different amounts of HeLa S3 cDNA. Data obtained under the indicated transfection conditions are shown as relative, normalized expression levels. Total RNA was prepared using the RNeasy Mini Kit and the QIAShredder homogenizer.
High-quality siRNA for efficient gene silencing


QIAGEN offers two grades of custom siRNA; ultrapure siRNA purified by ion-exchange HPLC (>97% pure), and crude siRNA (8085% pure). In addition, a number of library siRNA duplexes (>97% pure) are available for silencing common target genes. All siRNA products are delivered annealed,* desalted, deprotected, and ready to use. A detailed certificate of a
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