Navigation Links
Custom and library siRNA for efficient gene silencing

sgenic animals and plants, detection of bacteria and viruses, and microsatellite analyses
  • Easy to use and cost-effective - simple reaction setup for fast and reproducible result Principle of siRNA-mediated RNA interference

    The application of RNAi technology to mammalian cells has revolutionized the field of functional genomics. Use of siRNA allows targeted inhibition of gene expression using double-stranded RNA (dsRNA) that carries the same sequence as mRNA transcribed from the target gene. In mammalian cell systems, these double-stranded siRNA oligos can be used to induce gene silencing. Targeting a specific gene by RNAi requires a double-stranded siRNA containing the sense and antisense sequence of mRNA transcribed from the target gene. QIAGEN uses patented TOM-amidites to synthesize high-quality, highpurity, 21-nucleotide sense and antisense RNA oligonucleotides. In mammalian cells, siRNA must be delivered to the cytoplasm using an RNA transfection reagent. TransMessenger Transfection Reagent from QIAGEN efficiently delivers siRNA to cells for successful gene knock down (Figures 1 and 2). Once inside the cell, current models suggest that siRNA interacts with endogenous cellular proteins to form a proteinRNA complex known as the RNA induced silencing complex (RISC). Due to the sequence homology between the siRNA and the target mRNA, the RISC specifically targets the mRNA for enzymatic degradation (see flowchart).

    Efficient Gene Silencing Using QIAGEN siRNA and TransMessenger Reagent siRNA-Mediated RNAi
    '"/>


  • Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Custom and library siRNA for efficient gene silencing
    2. Custom siRNA Oligo Synthesis Service
    3. Ambion Custom
    4. Ambion Custom: Made-to-Order
    5. Custom RNA & RNA Isolation Services to Meet Your Specific Needs
    6. Cancer siRNA Oligo Set Version 1.0
    7. Library siRNA
    8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
    9. Quantification of siRNA Silencing Efficiency Using the LightCycler System
    10. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
    11. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Custom and library siRNA for efficient gene silencing

    (Date:8/29/2014)... Albany, New York (PRWEB) August 29, 2014 ... be valued at $5.6 billion in 2019. It ... 18.1% from 2013 to 2019, and was valued ... research report published by Transparency Market Research. , ... please visit: http://www.transparencymarketresearch.com/companion-diagnostics-market.html . , The research ...
    (Date:8/29/2014)... PA (PRWEB) August 29, 2014 Pittcon ... Hub MacDonald, and colleague Koichiro Matsuda, Horiba Scientific, have ... on September 5, 2014. JASIS , Asia’s largest ... 3-5, 2014, in Makuhari Messe, Japan. , The ... Tools for Bioanalysis from Single Molecules to Single Cells” ...
    (Date:8/29/2014)... Local veterinarian, Dr. Keith Clement, and his ... to participate in an investigational study of donor stem ... of this study is to determine if a single ... arthritically affected joints can help reduce pain and inflammation ... current investigational study must be older than nine months, ...
    (Date:8/29/2014)... 29, 2014 Due to a misstatement posted ... the Adult Stem Cell Technology Center, LLC ( ASCTC ) ... cell therapy research that might benefit ALS patients. Because ... new stem cell technologies and therapeutic applications, FRC listed ASCTC ... Challenge donors who wish not to support research that ...
    Breaking Biology Technology:Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 3
    ... SAN FRANCISCO, Jan. 10, 2011 Enobia Pharma Inc., ... II study of ENB-0040, a bone targeted enzyme replacement ... serious, rare metabolic bone disorder. The results were presented ... of Enobia, at the 29th Annual J.P. Morgan Healthcare ...
    ... (Nasdaq: PRXL ) will release Second Quarter Fiscal ... the close of the stock market.  The announcement will be ... the PR Newswire website at www.prnewswire.com . ... 10:00 a.m. EST on Tuesday, February 1, 2011 to discuss ...
    ... DuPont (NYSE: DD ) has entered ... a global enzyme and specialty food ingredients company, for ... of Danisco net debt.  Upon closing, this transaction would ... with science-intensive innovations that address global challenges in food ...
    Cached Biology Technology:Enobia Announces Successful Completion of Phase II Juvenile Study of ENB-0040, a Bone Targeted Enzyme Replacement Therapy 2Enobia Announces Successful Completion of Phase II Juvenile Study of ENB-0040, a Bone Targeted Enzyme Replacement Therapy 3PAREXEL Announces Date of Second Quarter Fiscal Year 2011 Earnings Release and Conference Call 2DuPont to Acquire Danisco for $6.3 Billion 2DuPont to Acquire Danisco for $6.3 Billion 3DuPont to Acquire Danisco for $6.3 Billion 4
    (Date:8/28/2014)... MARC (Maximizing Access to Research Careers) Program has announced ... Human Genetics from October 18 22, 2014 in ... promote the entry of students, post doctorates and scientists ... science community and to encourage the participation of young ... This year MARC conferred 16 awards totaling $29,600., The ...
    (Date:8/28/2014)... identified the developmental on-off switch for Streptomyces , ... two-thirds of the world,s naturally derived antibiotic medicines. ... it is possible to manipulate this switch to make ... appearing August 28 in Cell , found that ... and a larger protein called BldD ultimately controls whether ...
    (Date:8/28/2014)... measuring and imaging how quickly blood flows in the ... drug abuse affects the brain, which may aid in ... better treatment options for recovering drug addicts. The new ... Brook University in New York, USA and the U.S. ... Optical Society,s (OSA) open-access journal Biomedical Optics ...
    Breaking Biology News(10 mins):Small molecule acts as on-off switch for nature's antibiotic factory 2Small molecule acts as on-off switch for nature's antibiotic factory 3This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3
    ... even a small amount of MDMA, better known as ecstasy, ... study to look at the neurotoxic effects of low doses ... were presented today at the annual meeting of the Radiological ... in blood circulation in some areas of the brain in ...
    ... India constitute more than one-sixth of the world's ... related to genetic diseases. And with the growth ... and western lifestyles have risen to near-epidemic proportions, ... importance. , In response to this dearth ...
    ... has yielded new evidence for domesticated horses up to 5,600 ... appear to be the remains of horse corrals beside pit ... by horse manure. The Krasnyi Yar site was inhabited by ... relied heavily on horses for food, tools, and transport. , ...
    Cached Biology News:Ecstasy can harm the brains of first-time users 2Researchers make progress in studying genetic traits of India-born populations 2New evidence of early horse domestication 2New evidence of early horse domestication 3
    ... conserved form of cell suicide, which follows ... component of this process is a cascade ... enzymes participate in a series of reactions ... signals and result in the cleavage of ...
    ... System provides optimized reagents and protocols for ... genes by insertion of group II introns. ... group II introns and utilizes a simple ... II intron for specific insertion into the ...
    Mouse monoclonal antibody raised against a full length recombinant PPIL2. NCBI Entrez Gene ID = PPIL2...
    Mouse monoclonal antibody raised against a partial recombinant QARS. NCBI Entrez Gene ID = QARS...
    Biology Products: