Navigation Links
Custom and library siRNA for efficient gene silencing

sgenic animals and plants, detection of bacteria and viruses, and microsatellite analyses
  • Easy to use and cost-effective - simple reaction setup for fast and reproducible result Principle of siRNA-mediated RNA interference

    The application of RNAi technology to mammalian cells has revolutionized the field of functional genomics. Use of siRNA allows targeted inhibition of gene expression using double-stranded RNA (dsRNA) that carries the same sequence as mRNA transcribed from the target gene. In mammalian cell systems, these double-stranded siRNA oligos can be used to induce gene silencing. Targeting a specific gene by RNAi requires a double-stranded siRNA containing the sense and antisense sequence of mRNA transcribed from the target gene. QIAGEN uses patented TOM-amidites to synthesize high-quality, highpurity, 21-nucleotide sense and antisense RNA oligonucleotides. In mammalian cells, siRNA must be delivered to the cytoplasm using an RNA transfection reagent. TransMessenger Transfection Reagent from QIAGEN efficiently delivers siRNA to cells for successful gene knock down (Figures 1 and 2). Once inside the cell, current models suggest that siRNA interacts with endogenous cellular proteins to form a proteinRNA complex known as the RNA induced silencing complex (RISC). Due to the sequence homology between the siRNA and the target mRNA, the RISC specifically targets the mRNA for enzymatic degradation (see flowchart).

    Efficient Gene Silencing Using QIAGEN siRNA and TransMessenger Reagent siRNA-Mediated RNAi
    '"/>


    • Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Custom and library siRNA for efficient gene silencing
    2. Custom siRNA Oligo Synthesis Service
    3. Ambion Custom
    4. Ambion Custom: Made-to-Order
    5. Custom RNA & RNA Isolation Services to Meet Your Specific Needs
    6. Cancer siRNA Oligo Set Version 1.0
    7. Library siRNA
    8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
    9. Quantification of siRNA Silencing Efficiency Using the LightCycler System
    10. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
    11. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    		TAG: Custom and library siRNA for efficient gene silencing

    (Date:12/2/2008)...FirstCall/ -- Bionovo, Inc.,(Nasdaq: BNVI ), a ...d,development of safe and effective drugs in the ...t Isaac Cohen, Chairman and CEO of Bionovo, will b...th Annual Piper Jaffray Health,Care Conference at...e held at,the New York Palace Hotel in New York C...
    (Date:12/2/2008)...Call/ -- Martek Biosciences Corporation (Nasdaq: ...esults of its fourth quarter and fiscal year 2008 ...astern Time (ET). Following the release, at 4:45 ...iscuss these results with investors. All interest... by visiting Martek,s web site at http://investor...
    (Date:12/2/2008)... Dec. 2 Enigma Diagnostics, the d...cs company, announced today the opening of a new U... Chris Melancon as Vice President, Business Develo... on December 1 and is Enigma,s first overseas faci...f rapid diagnostic instrument systems. Enigma,s ...
    (Date:12/2/2008)...- Starch Medical Inc, a San Jose,CA-based medica...f its,PerClot(TM) Polysaccharide Hemostatic Syste...aride Hemostat. Introduction of PerClot(TM) PHS w... other select international,markets. The StarFoam...h,during the 2nd quarter, 2009. , PerClot(...
    Breaking Biology Technology:Bionovo to Present at the Piper Jaffray Healthcare Conference 2Enigma Diagnostics Announces the Opening of a United States Office 2Enigma Diagnostics Announces the Opening of a United States Office 3Starch Medical Launches New, Bioinert Surgical Hemostats 2
    ... Since the late 1970s, the problem of storing fil...unds has proved an elusive challenge for the medic...,000 pictures per procedure, old methods of storin...e fast becoming outdated as the use of digital med...Wauwatosa-based startup with roots in the Mayo Cli...
    ...ion SOS MILWAUKEE IT Fusion SOS (Small Office Sol...viding managed service programs for its initial cl...ers, announced Tuesday, is based around an outsour... end-user a single point-of-contact for their LAN,...ting needs. This turnkey solution is designed for ...
    ...s Technologies recently announced it received a P...rant for $400,000 from the National Cancer Instit...fficer Barbara Israel, is to develop a new method ...e relevant to cancer biology. Using a liquid cryst...way to quickly quantify enzyme activity that could...
    ...ly released Q2 MoneyTree Survey figures show no re...tween April and June of this year. Both total vent...eported in the survey, which was released by Price...he National Venture Capital Association. Ten other...est Coasts collected the most money, according to ...
    Other Biology Technology:TeraMEDICA offers seamless sharing of digital medical images 2TeraMEDICA offers seamless sharing of digital medical images 3TeraMEDICA offers seamless sharing of digital medical images 4TeraMEDICA offers seamless sharing of digital medical images 5MoneyTree Survey reports no venture investment activity in Wisconin in Q2 2MoneyTree Survey reports no venture investment activity in Wisconin in Q2 3MoneyTree Survey reports no venture investment activity in Wisconin in Q2 4MoneyTree Survey reports no venture investment activity in Wisconin in Q2 5MoneyTree Survey reports no venture investment activity in Wisconin in Q2 6
    (Date:12/1/2008)...re , The Geological Society of America,s e-journal...gration from multiple fields, including tectonics,... study the southwestern U.S. climate 17 million ye... piggyback basin; Angel Lake orthogneiss in the Ea...h Balkan extensional system within southern Bulgar...
    (Date:12/1/2008)...esearch leading to the development of flood-tolera...eas worldwide, Julia Bailey-Serres of UC Riversi... of the International Rice Research Institute in ...ment of Agriculture (USDA) with the 2008 USDA Nati...three scientists are, or have been, principle inve...
    (Date:12/1/2008)...asic molecules that make up all living things have...r to the way people are right- or left-handed. Thi...try and molecular interactions of living organisms...uilding blocks of matter is one of the great myste.... Department of Energy,s Argonne National Laborato...
    (Date:12/1/2008)...rnal of the American Dietetic Association feature... habits of consumers. Researchers look at why fast...thods for adding healthier foods to a person,s die...urnal of the American Dietetic Association include...easons among Frequent Consumers, Menu Modeling wi...
    Breaking Biology News(10 mins):December Geosphere media highlights 2December Geosphere media highlights 3UC Riverside rice geneticist receives high honor from US Department of Agriculture 2UC Riverside rice geneticist receives high honor from US Department of Agriculture 3Argonne scientists discover possible mechanism for creating 'handedness' in biological molecules 2News from the December 2008 Journal of the American Dietetic Association 2Molecules can block breast cancers ability to spread 9331 1Molecules can block breast cancers ability to spread 9331 2Radioactive understudy may aid medical imaging drug development 1830 1Radioactive understudy may aid medical imaging drug development 1830 2Disrupting common parasites ability to talk to each other reduces infection 1827 1Disrupting common parasites ability to talk to each other reduces infection 1827 2Disrupting common parasites ability to talk to each other reduces infection 1827 3Surprise cholesterol may actually pose benefits study shows 1824 1Surprise cholesterol may actually pose benefits study shows 1824 2
    ... the time of Ghengis Khan could form the basis of ...is a known source of biologically active chemicals...for health drinks and various cosmetics. Juice ext...ut scientists in India have developed a highly eff...olesterol-lowering compounds. Two companies have s...
    ... in the war against flu, researchers at the Univer...ery of a novel compound that confers broad protect...ian influenza. , The new work, reported online thi...describes the discovery of a peptide -- a small pr...uenza virus from attaching to and entering the cel...
    ...experiment turned out to be anything but for bacte...out why what should have been a routine procedure ... creation of a new biological tool for destroying ...rigger antibiotic resistance. , The discovery al...logy firm that has already brought Wisconsin a doz...
    ... through the human body in Fantastic Voyage, docto...lls after a transplant, or to find where and how m...ell. New technology described in a study published...gy makes it possible to image and quantify molecul... Claude Lechene and colleagues describe the develo...
    Other Biology News:New drug blocks influenza, including bird flu virus 2New drug blocks influenza, including bird flu virus 3'Failed' experiment yields a biocontrol agent that doesn't trigger antibiotic resistance 2'Failed' experiment yields a biocontrol agent that doesn't trigger antibiotic resistance 3Fantastic Voyage: A new nanoscale view of the biological world 2
    Mouse H60 Allophycocyanin MAb (Clone 205326) ENTREZ GeneID: 15101
    Can be used for the quantitative recovery of small amounts of nucleic acids within dilute solutions. 5mg/ml
    Recombinant Mouse sTNF RII/TNFRSF1B
    Human Norrin Biotinylated Affinity Purified PAb Protein Family: Other Growth Factors, Other Wnt-related Molecules
    Biology Products: