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Custom and library siRNA for efficient gene silencing

sgenic animals and plants, detection of bacteria and viruses, and microsatellite analyses
  • Easy to use and cost-effective - simple reaction setup for fast and reproducible result Principle of siRNA-mediated RNA interference

    The application of RNAi technology to mammalian cells has revolutionized the field of functional genomics. Use of siRNA allows targeted inhibition of gene expression using double-stranded RNA (dsRNA) that carries the same sequence as mRNA transcribed from the target gene. In mammalian cell systems, these double-stranded siRNA oligos can be used to induce gene silencing. Targeting a specific gene by RNAi requires a double-stranded siRNA containing the sense and antisense sequence of mRNA transcribed from the target gene. QIAGEN uses patented TOM-amidites to synthesize high-quality, highpurity, 21-nucleotide sense and antisense RNA oligonucleotides. In mammalian cells, siRNA must be delivered to the cytoplasm using an RNA transfection reagent. TransMessenger Transfection Reagent from QIAGEN efficiently delivers siRNA to cells for successful gene knock down (Figures 1 and 2). Once inside the cell, current models suggest that siRNA interacts with endogenous cellular proteins to form a proteinRNA complex known as the RNA induced silencing complex (RISC). Due to the sequence homology between the siRNA and the target mRNA, the RISC specifically targets the mRNA for enzymatic degradation (see flowchart).

    Efficient Gene Silencing Using QIAGEN siRNA and TransMessenger Reagent siRNA-Mediated RNAi
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