( Signal Intensity ...)Critical factors for successful microarray and real-time PCR analyses

Signal Intensity SensiChip System Reduces Background Figure 4 The HiLight Detection System uses biotin-labeled cDNA and HiLight RLS Particles SC coated with anti-biotin antibodies. The intense non-fluorescent signals enable simple and highly sensitive
detection of nucleic acids on microarrays. Figure 5 PWG technology provides excitation that is strictly confined to the
array surface. This results in high signal-to-noise ratios and improved sensitivity.

Validation of gene expression pattern with highly specific and sensitive quantitative real-time RT-PCR

The state-of-the-art technology for confirmation and quantitative analysis of expression levels is quantitative, real-time RT-PCR. QuantiTect Kits from QIAGEN provide high sensitivity and specificity in real-time PCR and RT-PCR. Optimized, ready-to-use master-mix solutions eliminate tedious optimization procedures, and the kits can be used on any real-time PCR instrument.

Quantitative real-time RT-PCR using the QuantiTect Probe RT-PCR Kit confirms the RNA abundance levels determined with the SensiChip System and the HiLight Detection System and provides highly accurate quantification. Signal-to-noise ratios in the array experiments correspond with expected
gene expression levels (Figure 6A and 6B), which also correlate with the precise qu
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