Navigation Links
Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

. Dose curves for the enzyme inhibitor and a control sample value obtained with TopCount were compared with those from the Packard Tri-Carb 2250CA.

A sandwich immunoradiometric assay (IRMA) for TSH was performed in a solvent resistant PicoPlate or in microplate strips (Dynex Technologies) coated with anti-TSH MAb as the first antibody. An 125Ilabeled anti-TSH MAb was used to quantitate TSH bound to the solid support by the first antibody. The PicoPlate was counted in the TopCount with 250 μL MicroScint-O, and individual wells of the microplate strip were counted in a Packard Cobra gamma counter for comparison.

Results and Discussion

The average efficiencies and backgrounds obtained with TopCount with a small sample load are presented in Table 1. A small sample (10 μL) was used to provide the optimum cocktail performance. Counting efficiencies for 3H are comparable to those which can be obtained by conventional liquid scintillation counting.

The CPM results for equal volumes of 3H labeled samples in heptane counted with TopCount and a conventional LSC are compared in Figure 1. The data show that the TopCount correlates very well with the LSC (R2= 0.99). The slope of the line indicates that for this sample load TopCount CPM is approximately 40% of the CPM obtained by conventional LSC. This lower efficiency is a result of the much higher sample load; only 250 μL of cocktail was used in TopCount versus 5 mL in the LSC.

Table 2 shows a comparison of the % inhibition values for a control sample obtained from TopCount with the value obtained by LSC. Assays 1-4 were counted with TopCount using four different sample volumes, and assay five was counted with the Tri- Carb 2250CA. These control values are additional confirmation that TopCount provides consistent results regardless
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
5. Direct Counting of Millipore MultiScreen Filtration Plates.
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
(Date:1/15/2014)... and SAN JOSE, California , ... biotechnology company developing antibody-drug conjugates for cancer, today announced the ... of Directors. Dr Reynolds has over 20 years, development experience ... Officer at Seattle Genetics. "I am delighted to ...
(Date:1/15/2014)... Toronto, Canada (PRWEB) January 15, 2014 ... perspectives on nonclinical and clinical safety assessment in biosimilars. ... a path for biosimilar drug development, however the complex ... regards to quality, safety and efficacy extremely challenging. Based ...
(Date:1/15/2014)... Houston, TX (PRWEB) January 15, 2014 ... Alzheimer’s disease, and about 1 in 3 seniors will ... ( source ). These jaw-dropping figures have shocked many ... function and, hopefully, help prevent these tragic age-related cognitive ...
(Date:1/15/2014)... The Microcompetition with Foreign DNA theory explains how latent viruses ... is the Epstein Barr Virus (EBV), and one of these ... chronic inflammatory disease that destroys the body’s joints. As predicted ... high concentrations of EBV DNA in their bodies (1). ...
Breaking Biology Technology:Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3
... unique, Rice University-based program that combines a traditional ... undergraduate research internship in nanotechnology, has been awarded ... Heiskell Award for Innovation in International Education. , ... and honor the most outstanding initiatives in international ...
... January 28 Nycomed has granted,Sepracor, Inc. ... marketing and,commercialisation rights for Ciclesonide in the ... a novel principle of action. It,is the ... for the,treatment of asthma and in the ...
... Joseph F. Finn, Jr.,CPA, the Assignee for the Benefit ... of the Company,s fixed assets for in,excess of $300,000 ... to,the high volume of requests for due diligence materials ... the Company has,created an online datasite that allows access ...
Cached Biology Technology:Rice wins innovation award for international program 2Nycomed's Ciclesonide to be Commercialised in the United States by Sepracor 2Nycomed's Ciclesonide to be Commercialised in the United States by Sepracor 3Nycomed's Ciclesonide to be Commercialised in the United States by Sepracor 4Nycomed's Ciclesonide to be Commercialised in the United States by Sepracor 5Nycomed's Ciclesonide to be Commercialised in the United States by Sepracor 6
(Date:4/17/2014)... to treat tuberculosis could be the basis for a ... fungal infections and parasites, yet evade resistance, according to ... , Led by U. of I. chemistry professor Eric ... SQ109 attacks the tuberculosis bacterium, how the drug can ... malaria and how targeting multiple pathways reduces the ...
(Date:4/17/2014)... NJ. April 16, 2014. Kessler Foundation has been ... million from the Department of Defense Spinal Cord ... principal investigator for the randomized, double-blinded, controlled, multi-site ... bone and muscle strength after spinal cord injury. ... & Engineering Research at Kessler Foundation. Two additional ...
(Date:4/17/2014)... per eight hundred births, Down syndrome - or trisomy ... intellectual disability. It results from a chromosomal abnormality where ... chromosome 21 (1% of the human genome). A study ... Department of Genetic Medicine and Development at the University ... Nature , shed light on how the extra chromosome ...
Breaking Biology News(10 mins):Multitarget TB drug could treat other diseases, evade resistance 2Kessler Foundation awarded Department of Defense grant for spinal cord injury research 2Trisomy 21: How an extra little chromosome throws the entire genome off balance 2Trisomy 21: How an extra little chromosome throws the entire genome off balance 3
... The common fruit fly circling your week-old peach has ... the insect,s eggshell formation. The paradoxical finding gives ... eggshell layer forms as it points to a likely ... biologist in the Medical College of Georgia Schools of ...
... plain with the odd hydrothermal vent, the seafloor appears ... paper being published May 29 in Nature. A ... mid-ocean ridge spreading system, representing potentially the largest surface ... geomicrobiologist Katrina Edwards, the studys corresponding author. While ...
... WEST LAFAYETTE, Ind. - Throughout the overlooked depths of ... important animal is rapidly disappearing. Until recently, the ... was a major food source for commercially important species ... salmon, trout and walleye rely. Scientists are employing ...
Cached Biology News:Fruit fly helps identify protein critical to eggshell formation that may be pesticide target 2Fruit fly helps identify protein critical to eggshell formation that may be pesticide target 3'Barren' seafloor teeming with microbial life 2'Barren' seafloor teeming with microbial life 3A Great Lakes mystery: The case of the disappearing species 2A Great Lakes mystery: The case of the disappearing species 3A Great Lakes mystery: The case of the disappearing species 4
... (CMV) pp65 Antigenemia Immunofluorescence Assay (IFA) is ... matrix protein pp65 of CMV in isolated ... Erythrocyte Lysis Buffer - ... mL of buffered ammonium chloride. (NH4CL) ...
ANTI NO-TRYPTOPHAN CONJUGATE...
ANTI DEOXY CORTICO...
... both 0.2 and 2.0 mL samples for freezing point determinations. Includes built-in ... reading capability with optional scanner. RS-232 port. Microprocessor-controlled autocalibration. Two-step Calibration. ... ... ...
Biology Products: