Navigation Links
Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

ermined using 3H and 14C hexadecane for both the 96- and 24-well formats. To test the effects of sample load, varying volumes of samples in an organic solvent from an enzyme inhibition assay were counted in 96-well solvent resistant PicoPlates, and the results were compared to those obtained by conventional LSC. A TSH immunoradiometric assay was counted, and the results were compared to those obtained by gamma counting.

Experimental Methods

Counting efficiency was tested by adding 3H hexadecane to three wells of a 96-well PicoPlate containing 250 μL of MicroScint-O. For the 24-well PicoPlate, 3H was added to three wells containing 1.0 mL of cocktail. The same number of wells containing only cocktail were used for background determination. Similar experiments were carried out for 14C. The plates were sealed with Packards TopSealTM-P, a solvent resistant cover film, using the Packard MicroMate 496 Microplate Heat Sealer, and then mixed on a microplate shaker for 15 minutes. The samples were counted for five minutes each, under conditions optimized for liquid counting, on a TopCount with the VariPlateTM option which allows counting of either 96- or 24-well plates. To test the effects of sample load, 3H labeled samples in heptane solvent from an enzyme inhibition assay were counted using four different sample volumes. Aliquots of 25, 50, 100, and 150 μL in quadruplicate were pipetted into a solvent resistant 96-well Pico- Plate with a multiple-tip pipetter. Total volumes were brought to 300 μL with MicroScint-O. The plates were sealed and counted as described above. An additional 50 μL aliquot was counted for five minutes in a Packard Tri-Carb 2250CA liquid scintillation analyzer, using 5 mL Opti-Fluor organic cocktail in 7 mL glass vials, and the results were correlated with those from TopCount
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
5. Direct Counting of Millipore MultiScreen Filtration Plates.
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
(Date:3/31/2015)... , March 31, 2015  Bayer HealthCare ... Institute of Massachusetts Institute of Technology (MIT) and ... discovery. The goal of this new part of ... genetics to help create new cardiovascular therapies. ... with the Broad Institute to the area of ...
(Date:3/31/2015)... March 31, 2015  University of Illinois, ... will deliver the plenary session address on Monday, May ... Symposium (IMS) , kicking off a week of more ... the intersection of RF and microwave technologies with health. ... Radios, Sensors and Circuits for the Skin," will focus ...
(Date:3/31/2015)... 2015 Lighthouse Information Systems, Inc., ... in California, Oregon, Washington and clients across the ... and Acquisition (M&A) Integration Services offering, effective immediately. ... Services and M&A Integration Practice Leader at Lighthouse, ... majority of M&A initiatives fail to achieve their ...
(Date:3/30/2015)... -- Heidrick & Struggles (Nasdaq: HSII ), the premier provider ... culture shaping worldwide, today  announced that John ... Healthcare and Life Sciences practice. As Global Practice ... a team of more than 80 partners, principals and associates ... Asia serving clients in the biopharmaceutical, medical ...
Breaking Biology Technology:Bayer HealthCare Expands Collaboration with Broad Institute of MIT and Harvard to Develop Therapies for Cardiovascular Disease 2Bayer HealthCare Expands Collaboration with Broad Institute of MIT and Harvard to Develop Therapies for Cardiovascular Disease 3Health and Engineering Converge at the 2015 International Microwave Symposium 2Health and Engineering Converge at the 2015 International Microwave Symposium 3Lighthouse Information Systems, Inc., Announces Addition of M&A Integration Services 2Heidrick & Struggles Strengthens Leadership of Healthcare - Life Sciences Practice 2
... RO, ROG; Pink Sheets: RHHBY ) announced today that ... cobas s 201 system is now available in the ... and detect hepatitis A virus (HAV) simultaneously in one assay in ... treatments for life-threatening and chronic diseases and disorders such as hemophilia ...
... Chestnut Hill, Mass. (2/3/2011) -- Boston College has been ... Foundation to support a team of university researchers developing ... create images that reveal micro- and macroscopic matter with ... or NCOM, would join a new class of microscopes ...
... 2011 Ron Van Beek, president and CEO of ... Digestive Support for dogs and cats, available exclusively through ... digestive support supplement containing both probiotics and prebiotics that ... in a dog,s or cat,s GI tract.  Van Beek ...
Cached Biology Technology:First Duplex Test for Parvovirus B19 and Hepatitis A Virus Now Available in the US 2Boston College receives W.M. Keck Foundation funding for nanoscale optical microscope 2Van Beek Natural Science Announces New Synacore Digestive Support Product 2
(Date:3/24/2015)... --  NexID Biometrics LLC, whose spoof-mitigation technology boosts ... the beginning of shipments of version 2.0 of its ... based in Potsdam, N.Y. , is ... began here today at the Walter E. Washington Convention ... SDK boosts the accuracy rate range to 96.5 to ...
(Date:3/23/2015)... LOS ANGELES , March 23, 2015  In ... in Federal fraud case conviction losses. This figure accounts ... fraud, which, according to The Nilson Report, exceeds $11 ... an investigative tool after a crime has occurred. Investor ... Agent and polygraph expert Joe Paolella partnered ...
(Date:3/20/2015)... , March 20, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... is one of the products featured in 2015 "I ... at 11:00 PM EST on the DIY Network.   ... Las Vegas , site of the 2015 ...
Breaking Biology News(10 mins):NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2Polygraph Test Now Used to Protect Investors From Fraud 2Polygraph Test Now Used to Protect Investors From Fraud 3NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 2NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 3
... jellyfish to filigreed oak trees, each species in nature ... the evolutionary changes that produce the seemingly limitless diversity ... remains a mystery. Nevertheless, a better understanding of how ... sizes and shapes could shed light on diseases that ...
... WHITE PLAINS, N.Y., Feb. 24, 2012 -With the help of ... and Canada are seeking answers to questions such as how ... the causes of preeclampsia, and how air pollution may affect ... preventing preterm birth so more babies will get a healthy ...
... light isotopes vying for the coolest spots as magma turns ... by Case Western Reserve University shows. This tiny detail ... molten rock cools along a gradient, atoms want to move ... faster than cooler atoms and, therefore, hotter atoms move to ...
Cached Biology News:A biodiversity discovery that was waiting in the wings -- wasp wings, that is 2March of Dimes provides $3 million in new funding for preterm birth research 2March of Dimes provides $3 million in new funding for preterm birth research 3How heavy and light isotopes separate in magma 2How heavy and light isotopes separate in magma 3
... to significantly increase the shelf life of ... increased at room temperature storage conditions in ... is subjected to 0.45 micron filtration, and ... formulation insures consistently high levels of activity ...
... The StatMatic I provides a convenient and ... culture work. Used with an eight-port disposable ... not possible with multi-channel pipettes. The eight-port ... in volumes of 100, 200, 250, or ...
...
... a dual strength adhesive system which facilitates the ... adhesives on either side of the frame are ... the glass has an easy release adhesive whereas ... a stronger bond. This means that when the ...
Biology Products: