Navigation Links
Counting FlashPlates on the Wallac MicroBeta

0

P-33, 175 - 960

Save the normalization protocol and then check the plate map. Two plates are required for FlashPlate normalization. The first one is an empty plate that is used for determining the background of each of the detectors. For example, a six-detector instrument will store six background values and subtract the appropriate value from each unknown - detector specific background subtraction. The second plate has the active sample in G11 (96-well plate) or M22 (384-well plate). It is advisable to acquire at least 10,000 counts for each reading.

Next, create a counting protocol. The key selections, Other, upper PM, and window setting must be identical to the normalization or the normalization will not be visible from within the counting protocol.

Now, when unknowns are selected, the CPM1 is the raw value and the CCPM1 is corrected for background and efficiency.

To avoid phosphorescence, both normalization plates and assay plates should be dark-adapted for a couple of minutes before counting.

FlashPlate is a registered trademark of Packard Instrument Company, exclusively licensed to PerkinElmer Life Sciences, Inc. FlashPlate is protected under US Patent 5,496,502 and foreign equivalents, to all of which PerkinElmer Life Sciences holds an exclusive worldwide license. TopCount is a registered trademark of Packard Instrument Co.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
4. Direct Counting of Millipore MultiScreen Filtration Plates.
5. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Counting FlashPlates the Wallac MicroBeta

(Date:7/24/2014)... OAK RIDGE, Tenn., July 24, 2014 -- A novel ... at the Department of Energy,s Oak Ridge National Laboratory ... known for its unusual physical and electrochemical properties. ... examined how oxygen affects the surface of a perovskite ... electronic behavior. The new avenue to understand surface behavior ...
(Date:7/24/2014)... sophisticated equipment, trained personnel, and detection dogs to ... attacks. A revolutionary new electronic chip with nano-sized ... much easier. , The groundbreaking nanotechnology-inspired sensor, devised ... ,s School of Chemistry and Center for Nanoscience ... Tracense, picks up the scent of explosives molecules ...
(Date:7/24/2014)... 2014 Research and Markets has ... Market 2014-2018" report to their offering.  ... is the in vitro growth of plant or animal ... and biopharmaceutical production. Cell culture is a crucial step ... and cell culture consumables are necessary for the in ...
(Date:7/24/2014)... Three companies from the angelMD network were ... Competition. Out of 700 competition entrants, Chiron Health, Litesprite, ... round. In this round twenty teams are invited to ... mentoring from thought-leaders and medical entrepreneurs from all over ... commitment to the Livestrong vision around the Big C ...
Breaking Biology Technology:ORNL study reveals new characteristics of complex oxide surfaces 2Nano-sized chip 'sniffs out' explosives far better than trained dogs 2Global Cell Culture Market 2014-2018: Key Vendors are Becton, Dickinson and Co, EMD, GE Healthcare, Lonza Group, Sigma Aldrich and Thermo Fisher Scientific 23 angelMD Startups Make Livestrong Big C Semi-finals 2
... Wisconsin's U.S. Senate campaign, Republican Tim Michels ... little more than an inconsequential sideshow. Michels said he ... uses adult stem cells or cells from umbilical cordsbut ... cells. , ,Technology is going to move us away ...
... Madison, Wis. Wisconsin could lose its carefully established ... Midwest if more money is not channeled into academic and ... Download the report (5.4 MB PDF) This ... advisor to the governor on technological matters. The report, titled ...
... businesses TeraMedica scans new market opportunity: smaller hospitals ... Platypus plans office move next June Ink ... more mobile computing Schneider buys GPS to track ... , UW-Extension gets $95,000 to help small businesses ...
Cached Biology Technology:It's 99 percent certain: Research cannot rely on adult stem cells alone 2It's 99 percent certain: Research cannot rely on adult stem cells alone 3Economic report raises red flags on Wisconsin's future 2Economic report raises red flags on Wisconsin's future 3Tech Digest: whats moving in the Midwest 2Tech Digest: whats moving in the Midwest 3
(Date:7/27/2014)... Scientists have discovered a new species of mayfly in the ... of India. In fact, this is the first time that ... been collected in peninsular India. , The new species, called ... T. Soldan for his substantial contribution to the understanding of ... authors of a study that describes the new mayfly in ...
(Date:7/27/2014)... "big data," a single computer cannot always find the ... distributed across a cluster of computers that analyze a ... mine your web history to present you with targeted ... favorite book or movie. But big data is about ... monitoring brain activity are generating unprecedented quantities of information. ...
(Date:7/25/2014)... burn scars on this false-color image from the Terra satellite ... year,s rash of wildfires in Eastern Russia. The burn ... green background. The wildfires have broken across the remote ... in this false-color image from the MODIS instrument, it ... fires that continue. Two recent image features noted below show ...
Breaking Biology News(10 mins):New tools help neuroscientists analyze 'big data' 2New tools help neuroscientists analyze 'big data' 3New tools help neuroscientists analyze 'big data' 4New tools help neuroscientists analyze 'big data' 5
... by Dr. Ann F. Jacobson, associate professor in Kent ... may initially choose to postpone but ultimately undergo total ... patient education before and after the procedure. ... pre-and post operative experiences with total knee replacement surgery, ...
... release is available in Spanish . , A ... (USA) and Nakai (China), among others, have deciphered for the first ... to the cover of the last issue of the prestigious journal ... to the group of Molecular Genetics of the Department of ...
... fund four faculty research projects for spring 2008. ... a number of excellent proposals submitted in response ... for the program is administered by the OSU ... by royalties from OSU-licensed technologies. These funded projects ...
Cached Biology News:Research examines factors in delaying or declining total knee replacement surgery 2Scientists decipher fruit tree genome for the first time 2Scientists decipher fruit tree genome for the first time 3OSU technologies funded for development 2OSU technologies funded for development 3
Fluorescence Preserving Media, 100 mL...
Antibodies were affinity purified using epitopes specific to XLF immobilized on solid support....
...
... Clone/PAD: 3B8A10. Immunogen: ... the C-terminal region of ... Specific for the human ... Human (positive control: 293 ...
Biology Products: