( added and the unbound mass/DPM using ...)Counting FlashPlates on the Wallac MicroBeta

added and the unbound mass/DPM, using the known specific activity for the compound and the known efficiency of a LSC counter. The MicroBeta proved to read the opaque plate from the top as effectively as the clear well plate read from the top and bottom.

Instructions for counting FlashPlates on the MicroBeta. Software version 2.7

Create a new normalization

Select Other for the label

Select Background Sample

Click on Extended

Click on PMT Usage

Select Upper and click on OK

Change the window setting to:

H-3, 175 360

I-125, 175 530

C-14, 175 650

S-35, 175 650

P-33, 175 - 960

Click on OK

Click on New in the Plate/Filter section

Enter the name of the plate (i.e. FlashPlate). No other changes from the default protocol are necessary.

Important! Change the window setting after selecting the PMT usage. Otherwise the program will reset the window to 150 - 1024

Instructions for counting FlashPlates on the MicroBeta. Software version 3.0

Create a new normalization

Select Other for the label

Corrections Tab

Select Background sample

Other Tab

Select Upper tube only

Change the window setting to:

H-3, 175 360

I-125, 175 530

C-14, 175 650

S-35, 175 65
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