Navigation Links
Counting Cytostar-T scintillating microplates on the MicroBeta JET

200 μM kynurenic acid. The 0.1 μM domoate/ 200 μM kynurenic acid was added to the wells as one solution and the agonist induced response measured by counting Cytostar-T microplates for 20 secs/well over 10 cycles.

RESULTS

Figures 1 and 2 demonstrate the advantage of using the MicroBeta JET to add the agonist to the wells of Cytostar-T scintillating microplates. Using the MicroBeta JET, the induced response can be followed immediately after addition of the agonist. Adding the agonist manually results in a lag time where the initial response to the agonist cannot be measured, giving a significant difference in counts obtained for domoate in the first counting cycle. The lag time can be overcome using the MicroBeta JET to perform additions of the agonist to the wells of the Cytostar-T scintillating microplates.

The immediate effect of adding the antagonist kynurenic acid is shown in Figure 3. Suppression of the agonist induced response was immediately seen on addition of the domoate/kynurenic acid solution to the wells of Cytostar-T scintillating microplates. This was shown as a decrease in signal which was dependent on the concentrations of agonist and antagonist used in the assay.

Manual addition of kynurenic acid would give a similar delay in the initial response, as shown in Figure 2, resulting in a significant proportion of the antagonist effect of kynurenic acid being lost in that time.

CONCLUSION

Addition of reagents to the wells of the Cytostar-T microplate using the MicroBeta JET allows the response generated by addition of both agonist and antagonist to be measured immediately. Previously, on addition of these reagents manually, it was not feasible to obtain several measurements within a 5 minute time period, which resulted in a lag time where it was not p
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting FlashPlates on the Wallac MicroBeta
3. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
4. Direct Counting of Millipore MultiScreen Filtration Plates.
5. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA
Post Your Comments:
(Date:4/24/2015)... 24, 2015 DryLet, LLC a ... applications such as animal waste reduction, bioremediation, wastewater ... in wastewater treatment plants and restaurant kitchen settings, ... the National Restaurant Association’s NRA Show 2015, in ... of the company will be available to share ...
(Date:4/24/2015)... 2015  Navitas Life Sciences is delighted to announce the ... and Regulatory Services. Shalabh is a proven leader in Life ... of Kinapse, as well as a Strategy consultant at Accenture. ... http://photos.prnewswire.com/prnh/20150423/201261LOGO ... am delighted to welcome Shalabh into this critical role and ...
(Date:4/23/2015)... CA (PRWEB) April 23, 2015 Athena ... personal development of executive women in San Diego’s technology ... the 17th Annual Pinnacle Awards at their Gala on ... Hyatt Hotel. Register now at http://athenasd.org/events/ , ... support the Athena San Diego vision of fostering networking, ...
(Date:4/23/2015)... 2015 Six startups from Russia ... 4-17 as part of the U.S.-Russia Innovation Corridor ... of biotechnology startups to participate in the program ... and the University of Maryland (UMD) signed a ... respective universities in the biomedical industry in 2013. ...
Breaking Biology Technology:DryLet to Showcase Bio-React at the National Restaurant Association’s NRA Show 2015 May 16-19, 2015 in Chicago, Illinois 2Shalabh Kumar Joins Navitas Life Sciences to Lead their Clinical and Regulatory Services Teams 2Athena Pinnacle Awards to Honor Executives, Corporations and Educators Working to Promote Women Leaders 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 3U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 4
... Canopus,BioPharma, Inc. (OTC: CBIA), has confirmed the ... reducing agent and has also demonstrated the,synergistic effect ... and,cetuximab (Erbitux) in two mice lung cancer models., ... stated, "We at,Canopus are encouraged with the discovery ...
... Dominica, June 19 Neuftec,Limited, the proprietor of ... recently issued a claim against Oxonica Energy,Limited, a ... of its,European Patent in the High Court of ... AGM Statement issued by Dr Matthews,(CEO) on 19 ...
... biopharmaceutical industry information on ... ... BioSpace, the world,s,leading online community for the bioscience industry, released ... devices industries in Asia. AsiaBio(TM) features dynamic,informational Hotbed Web pages ...
Cached Biology Technology:CB1400, Patented by Canopus BioPharma, Prevents Tumor Growth and Enhances the Anti-Tumor Effects of Cisplatin and Cetuximab (Erbitux). 2Neuftec Responds to a Fundamental Inaccuracy in Oxonica PLC's Regulatory Announcement 2BioSpace Announces New Online Community for Asia 2
(Date:4/10/2015)... 2015 Research and Markets ( ... "Security Competitive Profiles - NEC" report to their ... NEC will continue to supply a range of IT ... a company focus on the development of a Big ... opportunities in the Asia-Pacific region ...
(Date:4/2/2015)... , April 2, 2015 At its ... Salt Lake City , the American College of ... five new directors to its Board.  Members of the ... ACMG and for forming and advancing its policies and ... genetics profession. "It,s an eventful time in ...
(Date:3/31/2015)... March 31, 2015  Elephant Talk Communications Corp. (NYSE ... provider of Software Defined Network Architecture (ET Software DNA® ... revenue of approximately $20.4 million for the year ended ... million for the year ended December 31, 2013. ... Company had completed its multi-year transition away from its ...
Breaking Biology News(10 mins):NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... has found that species living together are not forced ... challenging a theory that has held since Darwin,s Origin ... the most diverse bird families in the world, the ... of the processes causing species differences to evolve. ...
... , GAINESVILLE, Fla. --- Using the largest dated evolutionary ... suggests how plants developed traits to withstand low temperatures, ... bigger threat than initially thought to plants and global ... the journal Nature and co-authored by University ...
... scientists, chemical engineers and physicists from the University of Pennsylvania ... crystals as a medium for assembling structures. In their ... in the repeating patterns found in liquid crystals, in nanoscale ... pattern out of an even simpler template: a three-dimensional array ...
Cached Biology News:'Be different or die' does not drive evolution 2'Be different or die' does not drive evolution 3Study: Some plants may not adapt quickly to future climate change 2Study: Some plants may not adapt quickly to future climate change 3Penn researchers grow liquid crystal 'flowers' that can be used as lenses 2Penn researchers grow liquid crystal 'flowers' that can be used as lenses 3