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Counting Cytostar-T scintillating microplates on the MicroBeta JET

or Modules installed for one or two injection and counting sequences. The single detector JET can have up to four Injector Modules.

Multi-detector JETS can be set to deliver different reagents to the reading position of each detector and therefore specific wells in each plate. For example, in the case of a six detector JET fitted with one injector module, up to six different reagents can be dispensed across each plate.

Counting control includes the facility for repeat measurements of each sample after injection. In this fashion kinetic analysis of reactions lasting seconds or longer can be studied.

In terms of instrument design, if reagent injection is selected in any particular counting protocol, an Injector Assembly is automatically positioned between the sample plate and the upper PM tubes. This injector assembly houses the reagent tubes and fiber optic light guides. If reagent injection is not necessary, performance of the MicroBeta JET is identical to the MicroBeta TriLux.

Assays based on Amersham Pharmacia Biotechs Cytostar-T scintillating microplates are highly suitable for MicroBeta JET. This plate allows the real time analysis of a broad spectrum of cell associated phenomena to be studied over a defined period of time.

EXPERIMENTAL

Cell based assays were set up using Cytostar- T scintillating microplates to determine the effect of selective agonists and antagonists on cloned GluR6 receptor channels expressed in BHK cells. Glutamate gated ion channels are permeable to cations such as Na+, K+ and Ca2+, therefore allowing [14C] guanidinium (a marker for Na+ ions) to be used in ion flux experiments. The use of the sodium channel blocker, procaine, allows the influx of [14C] guanidinium into the cells through the GluR6 receptor ch
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