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Counting Cytostar-T scintillating microplates on the MicroBeta JET

L. Smith1, M.J. Price-Jones1, K.T. Hughes1 and N.R.A. Jones2 (1 Nycomed Amersham plc., Forest Farm, Whitchurch, Cardiff, CF4 7YT, Wales, UK, 2 PerkinElmer Life Sciences, P.O.Box 10, FIN-20101, Turku, Finland)

INTRODUCTION

MicroBeta JET is a multi-detector instrument designed for liquid scintillation (LS) or luminescence detection of samples in microtitration plates, tubes or on filters.

Three different MicroBeta models are available. Two manually loaded models have sample capacities of up to 16 or 32 plates. The third model is fitted with an external loading platform for fully automatic loading by robot systems.

MicroBeta JET has either 1, 2, 3 or 6 detectors and can count in either 96 or 24 formats. Each MicroBeta detector comprises two photo-multiplier tubes (PMTs), one above the sample and one below. This arrangement provides the best possible counting geometry for every assay type.

For routine LSC, coincidence counting is a robust and sensitive method. Coincidence counting is independent of the type of scintillator and is unaffected by changes in temperature. In an ideal sample tritium efficiency is in excess of 55% whilst the background is typically less than 10 CPM.

In the case of measurement of Scintillation Proximity Assay (SPA) or Cytostar-T scintillating microplates ParaLux Count Mode is recommended because this maximizes the advantage of the lower PMT. In these assays the radio-label is much closer to the lower PMT and as a consequence considerably higher counting efficiencies can be obtained. ParaLux has also been shown to give superior color quench correction1.

The novel feature in JET is the inclusion of multiple reagent injectors for measuring prompt (or flash) reactions. Multi-detector JETS can have one or two Inject
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