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Counting Colonies or Plaques Directly from Images of Agar Plates

easily. Either one or two color groups can be analyzed, which allows scientists using color-selection techniques to count both blue and white colonies (or blue and clear plaques) separately and concurrently (figure 1 and figure 2). In order to speed analysis, the counting region can be determined automatically based on sharp differences in pixel intensity, such as the differences that occur along the edges of a petri dish. Initially, counting begins automatically after the counting region has been delineated. If preferred, a region can be defined manually from an Advanced Options dialog box. Scientists also have the ability to exclude text written on plates without having to physically erase the text before imaging. Counting results can be modified by manually adding or removing individual colonies or plaques. During automatic colony or plaque counting, users should visually compare colonies or plaques present within the image of the plate to those being reported. If counted colonies or plaques do not correlate well with those on the image, counting parameters may need adjustment. Several adjustable counting parameters are provided to obtain accurate results for many types of bacterial or plaque plates.

Adjustable Counting Parameters Provide Flexibility and Accuracy

EagleSight softwares Colony/Plaque Counting function allows users to choose from several options for defining background. During counting, the colony or plaque vicinity is surveyed and an appropriate background value is assigned. In this way, each colony or plaque has a background value that corresponds to its location. The size of the colony or plaque vicinity is adjustable, providing the flexibility needed to accurately count colonies or plaques from images with
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