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Corynebacterium glutamicum

Electroporation of cells:

  1. Add 1-2 l plasmid DNA (in water) to a minimum of 40 l of electrocompetent cells. Homogenize by gently mixing
    with pipette several times. Transfer mixture into a prechilled cuvette.
  2. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V) 2,500 V Time constant (T) 5 ms
  4. Immediately transfer cell suspension into LBG medium at a 1:25 dilution, incubate 1 hour at 30 C.
  5. Plate cells on selective plates; incubate for 4 days.
Expected Results: Transformation efficiency up to 5 x 105 transformants/g of DNA.


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