( Proven siRNA Controls and Matche...)Control Your siRNA Research

Proven siRNA Controls and Matched Primary Antibodies

Gene Specific siRNA Controls
Whether you are starting out in siRNA research or you are an siRNA expert, you'll appreciate the convenience of the Silencer gene specific siRNA controls. Each Silencer gene specific siRNA control contains 50 g of ready-to-use chemically synthesized and gel purified GAPDH, cyclophilin, c-myc, or -actin siRNA, which is sufficient for 165 transfections (24 well plates). Also included are 25 g of a scrambled Negative Control siRNA and a detailed Instruction Manual. Each control is validated for use in human cell lines and each lot is functionally tested in an siRNA experiment. The GAPDH and cyclophilin siRNAs are also validated for use in mouse cell lines. These siRNA controls are ideal for developing and optimizing siRNA experiments.

Monitoring Transfection Efficiency
Because low transfection efficiency is the most frequent cause of unsuccessful gene silencing experiments, optimizing transfection conditions is critical. The Silencer Gene Specific siRNA Controls are ideal for this application, particularly when they are fluorescently labeled using the simple procedure provided with the Silencer siRNA Labeling Kits. Once transfected into cells, uptake of the labeled siRNA can be correlated to gene silencing by fluorescence microscopy using one of Ambion's new primary antibodies matched to our gene specific siRNA controls (Figure 1).

Figure 1. Characteristi
'"/>

Source:

Page: All 1 2 3
Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Human t-PA Control Primer Set
4. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
5. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
6. Designing Controls for siRNA Experiments
7. RNAi: The Controls You Need
8. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
9. Long-Term Instrument Control and Connectivity Solutions
10. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
11. Custom and library siRNA for efficient gene silencing