Navigation Links
Control Your siRNA Research

Proven siRNA Controls and Matched Primary Antibodies


Gene Specific siRNA Controls
Whether you are starting out in siRNA research or you are an siRNA expert, you'll appreciate the convenience of the Silencer gene specific siRNA controls. Each Silencer gene specific siRNA control contains 50 g of ready-to-use chemically synthesized and gel purified GAPDH, cyclophilin, c-myc, or -actin siRNA, which is sufficient for 165 transfections (24 well plates). Also included are 25 g of a scrambled Negative Control siRNA and a detailed Instruction Manual. Each control is validated for use in human cell lines and each lot is functionally tested in an siRNA experiment. The GAPDH and cyclophilin siRNAs are also validated for use in mouse cell lines. These siRNA controls are ideal for developing and optimizing siRNA experiments.


Monitoring Transfection Efficiency
Because low transfection efficiency is the most frequent cause of unsuccessful gene silencing experiments, optimizing transfection conditions is critical. The Silencer Gene Specific siRNA Controls are ideal for this application, particularly when they are fluorescently labeled using the simple procedure provided with the Silencer siRNA Labeling Kits. Once transfected into cells, uptake of the labeled siRNA can be correlated to gene silencing by fluorescence microscopy using one of Ambion's new primary antibodies matched to our gene specific siRNA controls (Figure 1).

Figure 1. Characteristi cs of Ambion's Primary Antibodies for siRNA Research.


Primary Antibodies Matched to Silencer Gene Specific Controls
Ambion provides mouse monoclonal antibodies for the detection of GAPDH, c-myc, and -actin by immunofluorescence. Each of these primary antibodies has been used in immunofluorescence experiments at Ambion to detect the reduction in protein levels induced by siRNA. An example of such an experiment is shown in Figure 2. (Antibodies are supplied in solution in a 100 g unit size and are validated for use in immunofluorescence experiments. Note that the concentration and volume will vary by antibody).


Figure 2. Following the Silencing of -actin. An siRNA targeting -actin was labeled with Cy3 using the Silencer siRNA Labeling Kit. The labeled siRNA was transfected into HeLa cells and cells were analyzed 96 hours later. Green: -actin protein detected with anti--actin (Ambion) and a fluorescein labeled secondary antibody. Red: Cy3 labeled siRNA. Blue: DAPI stained nuclei. (Cy3 is a trademark of Amersham Biosciences.)


back to top


Ordering Information
Cat# Product Name Size 1632 Silencer siRNA Labeling Kit - Cy3 65 g labeled siRNA 1634 Silencer siRNA Labeling Kit - FAM 65 g labeled siRNA 4300 anti-GAPDH, mouse monoclonal 6C5 100 g 4302 anti-beta-actin, mouse monoclonal AC-15 100 g
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Human t-PA Control Primer Set
4. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
5. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
6. Designing Controls for siRNA Experiments
7. RNAi: The Controls You Need
8. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
9. Long-Term Instrument Control and Connectivity Solutions
10. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
11. Custom and library siRNA for efficient gene silencing
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... ... June 27, 2016 , ... Newly ... technologies, services and solutions to the healthcare market. The company's primary focus is ... manufacturing, sales and marketing strategies that are necessary to help companies efficiently bring ...
(Date:6/27/2016)...   Ginkgo Bioworks , a leading organism ... today awarded as one of the World Economic ... most innovative companies. Ginkgo Bioworks is engineering biology ... world in the nutrition, health and consumer goods ... customers including Fortune 500 companies to design microbes ...
(Date:6/24/2016)... ... ... While the majority of commercial spectrophotometers and fluorometers use the z-dimension of 8.5 ... end machines that use the more unconventional z-dimension of 20mm. Z-dimension or ... the cuvette holder. , FireflySci has developed several Agilent flow cell product lines ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... is pleased to announce the launch of their brand, UP4™ Probiotics, into Target ... over 35 years, is proud to add Target to its list of well-respected ...
Breaking Biology Technology:
(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
(Date:3/23/2016)... , March 23, 2016 ... erhöhter Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender ... das Unternehmen mit SpeechPro zusammenarbeitet, um erstmals ... Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im Rahmen ...
(Date:3/21/2016)... 2016 Unique technology combines ... superior security   Xura, Inc. ... secure digital communications services, today announced it is working ... enterprise customers, particularly those in the Financial Services Sector, ... authentication within a mobile app, alongside, and in combination ...
Breaking Biology News(10 mins):